本文選題：DNA折紙　切入點：納米粒子　出處：《廣東藥科大學》2017年碩士論文　論文類型：學位論文

【摘要】：DNA折紙結(jié)構(gòu)的形狀十分多樣,幾乎可以被構(gòu)建成任意形狀。但與其它材料的納米粒子相比,DNA折紙結(jié)構(gòu)的穩(wěn)定性相對較差,在一定程度上限制了它的應(yīng)用。如果能以DNA折紙結(jié)構(gòu)為模板,制備出與其形狀相同的其它材料的納米粒子,則可將該納米結(jié)構(gòu)的尺寸和形狀優(yōu)勢與材料的穩(wěn)定性結(jié)合,以滿足對不同形狀不同材料納米粒子的需求。近年來,非法對中藥材進行人工染色的情況時有發(fā)生。在針對中藥材中添加色素的檢測方法中,拉曼光譜法因?qū)悠窡o損傷,且對樣品預(yù)處理的要求很低,甚至無需進行預(yù)處理,具有很大的優(yōu)勢。其中,表面增強拉曼散射(Surface-Enhanced Raman Scattering,SERS)光譜技術(shù)因具有很高的檢測靈敏度而被廣泛應(yīng)用。而表面增強拉曼散射的增強效應(yīng)與其活性基底的形狀有關(guān)。因此,尋找形狀多變、可控性強的納米粒子來做SERS基底十分必要。若將DNA折紙結(jié)構(gòu)與SERS基底結(jié)合起來,可極大促進表面增強拉曼光譜的發(fā)展。基于以上背景,本文首先對DNA折紙結(jié)構(gòu)在不同條件下的穩(wěn)定性進行了研究,為其在不同領(lǐng)域的應(yīng)用提供理論基礎(chǔ)。實驗結(jié)果表明,DNA折紙結(jié)構(gòu)在緩沖液中很穩(wěn)定,可長期保存,但受溫度的影響較大,只能在低于解離溫度的環(huán)境中存在;當把DNA折紙結(jié)構(gòu)固定在基底上并置于空氣中時,其穩(wěn)定性很差,不能長期保存,但此時其對溫度的耐受程度比較強,可耐受約200℃的高溫;另外,溶液中的鎂離子濃度對DNA折紙結(jié)構(gòu)的穩(wěn)定性起著關(guān)鍵作用,且在一定范圍內(nèi),隨著鎂離子濃度的增高,DNA折紙結(jié)構(gòu)逐漸出現(xiàn)團聚趨勢。其次,在DNA折紙結(jié)構(gòu)穩(wěn)定存在的前提下,本文發(fā)展出了一種以其為模板制作二氧化硅納米粒子的方法,通過一種助結(jié)構(gòu)導(dǎo)向劑的連接,在DNA折紙結(jié)構(gòu)的表面包裹一層二氧化硅,合成與其結(jié)構(gòu)形狀相同的二氧化硅納米粒子。通過該種方法可制備出形狀可控的納米粒子。本文采用上述方法合成了矩形、滾筒型、五角星形和四面體線框結(jié)構(gòu)的二氧化硅納米粒子,并通過原子力顯微鏡(AFM)、掃描電子顯微鏡(SEM)、透射電子顯微鏡(TEM)、能譜(EDS)、X射線衍射儀(XRD)、小角X射線散射儀(SAXS)等方法對粒子的性質(zhì)進行分析。結(jié)果表明,使用該方法所得粒子為核殼結(jié)構(gòu),且殼層二氧化硅為無定型的介孔結(jié)構(gòu)。在不同形狀的二氧化硅納米粒子外噴一層金,可將其作為SERS基底,對中藥中的檸檬黃和日落黃進行拉曼檢測。實驗結(jié)果表明,滾筒型的粒子的SERS效應(yīng)最強,可以其為SERS基底可對中藥中的非法添加色素進行拉曼檢測。
[Abstract]:The DNA origami structure shape is very diverse, almost can be constructed into any shape. But the nanoparticles and other materials compared to the stability of DNA origami structure is relatively poor, which limits its application to some extent. If the DNA origami structure as template, the preparation of nanoparticles with the shape of other materials of the same, then can be combined with the stability of the nano structure size and shape and material advantages, to meet the different needs of different shape material nanoparticles. In recent years, the illegal artificial coloring of the Chinese herbal medicines have occurred. The detection method for adding pigment in Chinese herbal medicine, Raman spectroscopy method without damage to the sample, and the sample pretreatment is very low, even without pretreatment, has great advantages. The surface enhanced Raman scattering (Surface-Enhanced Raman, Scattering, SERS) spectroscopy technology Because the operation has high detection sensitivity and is widely used. While the surface enhancement effect and active substrate shape of Raman scattering. Therefore, looking for the shape changing, nanoparticles controllability to SERS substrate is necessary. If the DNA origami structure and SERS substrate together, which can greatly promote the development of surface enhanced Raman spectroscopy. Based on the above background, this paper studies the stability of DNA origami structure under different conditions, and provide a theoretical basis for its application in different fields. The experimental results show that the DNA origami structure is very stable in buffer, can be stored for a long time, but is affected by temperature, can only exist in the below the dissociation temperature; when the DNA origami structure fixed to the substrate and placed in the air, its stability is very poor, can not be stored for a long time, but this time the tolerance temperature is relatively strong, Can withstand high temperature of about 200 DEG C; in addition, the magnesium ion concentration in solution plays a key role on the stability of DNA origami structure, and in a certain range, with the increase of the concentration of magnesium ions, DNA origami structure gradually appeared agglomeration trend. Secondly, on the premise of the existence of DNA origami stable structure, this paper developed a method of making the template for the silica nanoparticles, connected by a co structure directing agent, on the surface of DNA origami structure covered with a layer of silica, silica nanoparticles synthesis and structure of the same shape. Through this kind of method can be prepared. The rectangular shape controlled nanoparticles synthesized by this method. Drum type, Pentagram and tetrahedron frame structure of silica nanoparticles, and by atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy spectrum ( EDS), X ray diffraction (XRD), small angle X ray scattering (SAXS) properties and other methods of particle were analyzed. The results show that the method for using the particle obtained core-shell structure, and the shell of silica mesoporous structure of amorphous silica nano particles. In spraying a layer of different shapes gold, which can be used as SERS substrates, Raman detection of traditional Chinese medicine in lemon yellow and sunset yellow. The experimental results show that the strongest effect of SERS roller type particles, can the SERS base of Chinese herbal medicine illegally added pigment for Raman detection.

【學位授予單位】：廣東藥科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.37;R284

【參考文獻】

相關(guān)期刊論文 前10條

1 何秋菊;王麗琴;;拉曼光譜法鑒定文物及藝術(shù)品中染料的研究進展[J];光譜學與光譜分析;2016年02期

2 劉燕德;靳曇曇;;拉曼光譜技術(shù)在農(nóng)產(chǎn)品質(zhì)量安全檢測中的應(yīng)用[J];光譜學與光譜分析;2015年09期

3 劉宸;黃文倩;王慶艷;陳立平;趙春江;;拉曼光譜在食品無損檢測中的應(yīng)用[J];食品安全質(zhì)量檢測學報;2015年08期

4 李丹;呂狄亞;陳輝;陸峰;陳強;褚克丹;;紙基-表面增強拉曼光譜法檢測染色摻偽的紅花藥材[J];藥物分析雜志;2015年08期

5 竇穎;孫曉榮;劉翠玲;趙浩然;;基于拉曼光譜技術(shù)的面粉品質(zhì)快速檢測[J];食品科學;2014年22期

6 劉艷;張勤;呂志剛;范可青;;紅花中非法添加色素的薄層色譜快速鑒別法[J];中國實驗方劑學雜志;2014年18期

7 陳偉根;趙立志;彭尚怡;劉軍;周婧婧;;激光拉曼光譜應(yīng)用于變壓器油中溶解氣體分析[J];中國電機工程學報;2014年15期

8 益磊;孟祥松;周志美;孫全峰;;染色增重中藥材及飲片科學監(jiān)管技術(shù)方法探究[J];中國藥事;2014年03期

9 吳娟霞;徐華;張錦;;拉曼光譜在石墨烯結(jié)構(gòu)表征中的應(yīng)用[J];化學學報;2014年03期

10 劉照軍;韓運俠;楊蕊;程龍;;明代古墓葬壁畫顏料的顯微拉曼光譜分析[J];中國激光;2013年06期

，

本文編號：1581420