本文選題：大黃魚　切入點(diǎn)：脂質(zhì)　出處：《食品科學(xué)》2017年10期 　論文類型：期刊論文

【摘要】：應(yīng)用超高效液相色譜和電噴霧/四極桿-靜電場軌道阱的質(zhì)譜聯(lián)用技術(shù),分析大黃魚脂肪中甘油三酯的組成。采用Hypersil Gold C_(18)柱(150mm×4.6mm,2.1μm),流動(dòng)相為82%甲醇(含0.1%甲酸)溶液和18%異丙醇溶液,混合后添加0.05%乙酸銨溶液,以等度洗脫方式分離大黃魚脂肪中的甘油三酯組分,柱后流出液采用電噴霧離子源,以正離子模式采集一級質(zhì)譜分子離子和二級質(zhì)譜的碎片離子信息,然后與Lipidblast數(shù)據(jù)庫中甘油三酯信息進(jìn)行比對。研究結(jié)果顯示,超高效液相色譜和Q-Exactive高分辨質(zhì)譜儀聯(lián)用技術(shù)快速、準(zhǔn)確鑒定出大黃魚脂質(zhì)中含有28種甘油三酯。PoOL、Po SO、PPO、PPoO、PSO、MPO、POL、PoSA8種甘油三酯占總量的87.87%以上,其中PoOL(C_(16:1)、C_(18:1)、C_(18:2))相對含量最豐富,占35.261%。
[Abstract]:The application of ultra-high performance liquid chromatography and electrospray / quadrupole / electrostatic field orbital trap mass spectrometry, The composition of triglycerides in the fat of Pseudosciaena Crocea was analyzed. The Hypersil Gold CST18) column was used. The mobile phase consisted of 82% methanol (including 0.1% formic acid) solution and 18% isopropanol solution. After mixing, 0.05% ammonium acetate solution was added, the mobile phase consisted of 82% methanol (including 0.1% formic acid) solution and 18% isopropanol solution. Triglyceride components in fat of Pseudosciaena Crocea were separated by isometric elution. After the column, electrospray ion source was used to collect the molecular ions of the first order mass spectrometry and the fragment ion information of the second order mass spectrometry by positive ion mode. Then compared with the triglyceride information in the Lipidblast database, the results showed that the combination of ultra-high performance liquid chromatography and Q-Exactive high resolution mass spectrometer was rapid. 28 kinds of triglycerides, PPOP, PPOP, PPOO, PPOO, POLO, POLSA8 kinds of triglycerides were identified accurately in the lipids of Pseudosciaena Crocea, of which Poolia Cn. 16: 1: 1: 1: C: 1822) was the most abundant, accounting for 35.261% of the total amount of triglycerides in the lipids of Pseudosciaena Crocea, and accounted for 35.261% of the total amount of triglycerides in the lipids of Pseudosciaena Croceans.
【作者單位】： 杭州市農(nóng)業(yè)科學(xué)研究院;浙江工商大學(xué)環(huán)境科學(xué)與工程學(xué)院;
【基金】：國家自然科學(xué)基金青年科學(xué)基金項(xiàng)目(31301463)
【分類號】：O657.63;TS254.7
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本文編號：1576106