發(fā)布時間：2018-03-04 10:18

  本文選題：拉曼光譜　切入點：乳腺癌　出處：《紅外與激光工程》2017年04期 　論文類型：期刊論文

【摘要】：采用重復(fù)性及生物兼容性較好的二維納米銀膜作為氧合血紅蛋白表面增強(qiáng)拉曼散射光譜的(SERS)基底,利用具有較好匹配的近紅外激光作為激發(fā)光源,通過引起共振拉曼效應(yīng)分別對健康女性和女性乳腺癌患者的氧合血紅蛋白進(jìn)行SERS光譜研究。比較兩組氧合血紅蛋白平均SERS光譜,發(fā)現(xiàn)存在差異。利用SPSS軟件的主成分分析(PCA)方法和獨立變量T檢驗統(tǒng)計分析方法,發(fā)現(xiàn)兩組光譜在659、813和1 122 cm~(-1)拉曼頻移處的譜峰有著極顯著的區(qū)別。對主成分進(jìn)行歸屬分析,女性乳腺癌患者氧合血紅蛋白分子中,吡咯環(huán)的反對稱變形振動、對稱變形振動以及吡咯環(huán)的呼吸振動的強(qiáng)度、相對于健康女性顯著減少,從而造成圖譜譜峰有明顯差異。除此以外,SERS技術(shù)結(jié)合SPSS統(tǒng)計分析方法能夠較好地區(qū)分健康女性和乳腺癌患者的氧合血紅蛋白,從而有望發(fā)展成為一種新型的乳腺癌臨床診斷技術(shù)。
[Abstract]:Two-dimensional nano-silver films with good reproducibility and biocompatibility were used as SERS substrates of surface enhanced Raman scattering spectra of oxygenated hemoglobin, and near-infrared lasers with good matching were used as excitation light source. The SERS spectra of oxygenated hemoglobin in healthy women and women with breast cancer were studied by resonance Raman effect. The mean SERS spectra of oxygenated hemoglobin were compared between the two groups. Using the principal component analysis (PCA) method of SPSS software and the statistical analysis method of independent variable T test, it was found that the spectral peaks of the two groups of spectra at 659,813 and 1 122 cm ~ (-1) cm ~ (-1) were very different. In the oxygenated hemoglobin molecule of female breast cancer patients, the intensity of antisymmetric deformation vibration, symmetrical deformation vibration and respiratory vibration of pyrrole ring were significantly reduced compared with healthy women. In addition, SERS technique combined with SPSS statistical analysis can better distinguish oxygenated hemoglobin between healthy women and breast cancer patients. It is expected to become a new clinical diagnosis technique for breast cancer.
【作者單位】： 遵義醫(yī)學(xué)院物理學(xué)教研室;遵義醫(yī)學(xué)院附屬醫(yī)院檢驗科;遵義醫(yī)學(xué)院醫(yī)學(xué)與生物學(xué)研究中心;
【基金】：國家自然科學(xué)基金((81460204) 遵義醫(yī)學(xué)院碩士啟動基金(F-638)
【分類號】：R737.9;O657.37

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