本文選題：熒光傳感器　切入點：近紅外熒光探針　出處：《中國科學(xué)技術(shù)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：重金屬元素是一類常見的環(huán)境污染物,在飲用水和食品安全等方面需對其含量進(jìn)行嚴(yán)格監(jiān)控。其中銅是重金屬元素中一類特殊的存在,它是維持人體健康必要的微量元素,在人體組織更新代謝及維持神經(jīng)系統(tǒng)正常運轉(zhuǎn)等方面具有重要作用。人體內(nèi)銅的代謝異常會引起諸多病癥,例如Alzheimer癥、Parkinson癥、Wilson疾病等。因此發(fā)展一種具有高度靈敏性的銅離子快速檢測方法是一項非常有價值的工作。熒光檢測法由于其具有靈敏性高,選擇性好,設(shè)備價廉,操作簡單,能夠?qū)崿F(xiàn)快速檢測等優(yōu)點,近年來引起人們的持續(xù)關(guān)注,廣泛應(yīng)用在重金屬檢測方面。目前,已經(jīng)開發(fā)出包括量子點、金屬團(tuán)簇、碳點、有機染料等不同材料的熒光傳感器,用于銅離子的熒光檢測。其中,近紅外熒光傳感器具有對細(xì)胞更小的光損傷和較高的組織穿透性,能夠克服生物組織背景熒光的干擾,特別適用于細(xì)胞成像方面。本文采用具有近紅外發(fā)射的半花菁衍生物有機染料實現(xiàn)了對二價銅離子(Cu2+)的高選擇性及靈敏性檢測,并用其進(jìn)行了細(xì)胞內(nèi)Cu2+成像。本論文分為兩章,每章具體內(nèi)容如下:第一章:首先對熒光分析法進(jìn)行了系統(tǒng)闡述,包括基本原理和檢測機理;然后針對不同的機理,詳述了針對Cu2+的熒光分析法的研究現(xiàn)狀;接著對近紅外熒光技術(shù)進(jìn)行了闡述,并對不同近紅外熒光材料及其在生物領(lǐng)域內(nèi)的應(yīng)用進(jìn)行了概述;最后提出了論文選題及主要研究內(nèi)容。第二章:介紹了具有近紅外熒光發(fā)射的基于半花菁結(jié)構(gòu)的新型有機熒光傳感器(XL-Cu)的制備并對Cu2+進(jìn)行了檢測。該探針主要通過自身的鄰羥基咪唑結(jié)構(gòu)與銅離子進(jìn)行動態(tài)配位,并對分子自身的半花菁熒光團(tuán)產(chǎn)生影響,從而使半花菁部分的熒光降低。依照此現(xiàn)象,開發(fā)了一種檢測Cu2+的新方法,該方法以熒光信號的變化為基礎(chǔ),具有高度的選擇性,在水溶液中檢測限可達(dá)14nM(R2=0.9994)。另外,該探針的最大發(fā)射峰位于近紅外區(qū)域,對生物組織具有較小的光損傷和更深的穿透性,同時,其細(xì)胞毒性較低,因此適用于細(xì)胞成像。
[Abstract]:Heavy metal elements are a kind of common environmental pollutants, which need to be strictly monitored in drinking water and food safety, among which copper is a special kind of heavy metal elements, and it is a necessary trace element to maintain human health. It plays an important role in the regeneration and metabolism of human tissues and in maintaining the normal functioning of the nervous system. Abnormal copper metabolism in the human body can cause many diseases. For example, Parkinson's disease, Wilson's disease, etc. Therefore, it is very valuable to develop a highly sensitive method for the rapid detection of copper ions. The fluorescence detection method is simple because of its high sensitivity, good selectivity, cheap equipment and simple operation. It has attracted people's attention in recent years and has been widely used in heavy metal detection. At present, fluorescent sensors including quantum dots, metal clusters, carbon dots, organic dyes and other materials have been developed. The near infrared fluorescence sensor has smaller light damage to cells and higher tissue penetration, which can overcome the interference of background fluorescence of biological tissue. It is especially suitable for cell imaging. In this paper, the highly selective and sensitive detection of copper ion (Cu2) with half-cyanine derivatives with near infrared emission has been achieved. This paper is divided into two chapters. Each chapter is as follows: chapter one: firstly, the basic principle and detection mechanism of fluorescence analysis are described systematically, and then the different mechanisms are discussed. In this paper, the research status of fluorescence analysis for Cu2 is described in detail, and then the near infrared fluorescence technology is described, and the applications of different near infrared fluorescent materials in biological field are summarized. Chapter 2: the preparation of a novel organic fluorescence sensor with near-infrared fluorescence emission based on semi-cyanine structure and the detection of Cu2 are introduced. The structure of o-hydroxy imidazole is dynamically coordinated with copper ion. A new method for the detection of Cu2 is developed, which is based on the change of fluorescence signal and has high selectivity. In addition, the maximum emission peak of the probe is located in the near infrared region, which has less light damage and deeper penetration to biological tissue, and its cytotoxicity is low, so it is suitable for cell imaging.
【學(xué)位授予單位】：中國科學(xué)技術(shù)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

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本文編號：1564934