本文關(guān)鍵詞： 毛細(xì)管電泳 膠束電動(dòng)毛細(xì)管色譜 納米材料 西甲魚毒 苯胺 對(duì)甲苯胺　出處：《青島科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本文主要研究了納米材料在毛細(xì)管電泳在線富集方法上的應(yīng)用,由于納米材料具有生物相容性好、比表面積大、物理化學(xué)性質(zhì)穩(wěn)定等特點(diǎn),于毛細(xì)管在線富集方法上具有顯著作用。通過(guò)對(duì)納米材料的應(yīng)用,富集效果顯著提高。本文共分為三個(gè)章節(jié):第一章,主要對(duì)毛細(xì)管電泳技術(shù)進(jìn)行了介紹。首先,討論了毛細(xì)管電泳的發(fā)展及應(yīng)用,重點(diǎn)涉及毛細(xì)管電泳的幾種分離模式;然后,對(duì)毛細(xì)管電泳在線富集技術(shù)的發(fā)展進(jìn)行了概括;其次,展望國(guó)內(nèi)外毛細(xì)管電泳研究現(xiàn)狀,對(duì)一些在毛細(xì)管電泳發(fā)展過(guò)程中具有重要作用的創(chuàng)新進(jìn)行了介紹;最后,介紹了納米材料在毛細(xì)管電泳上的應(yīng)用,分別列舉了幾種具有重要特性的功能性納米材料以及在毛細(xì)管電泳分離方法上所起到的作用。第二章,基于外部磁場(chǎng)和納米金放大的毛細(xì)管電泳免疫分析超靈敏在線快速檢測(cè)雪卡魚毒。本章節(jié)提出了一種基于毛細(xì)管電泳分離與電化學(xué)免疫分析聯(lián)用的快速靈敏在線檢測(cè)CTX3C的方法。在這種三明治夾心結(jié)構(gòu)的的免疫實(shí)驗(yàn)中,將修飾有CTX3C抗體的磁性納米材料作為探針,同時(shí)將辣根過(guò)氧化物酶以及CTX3C抗體修飾到納米金顆粒表面上作為識(shí)別因子。通過(guò)在毛細(xì)管進(jìn)樣口外部設(shè)置一個(gè)外部磁場(chǎng)使免疫復(fù)合物在線合成得以實(shí)現(xiàn)。與傳統(tǒng)的高效液相色譜-質(zhì)譜以及酶聯(lián)免疫反應(yīng)方法相比,這種采用納米金作為多酶載體、基于外部磁場(chǎng)在線形成三明治結(jié)構(gòu)的免疫復(fù)合物的方法具有靈敏度高、分析時(shí)間短等特點(diǎn)。本方法檢測(cè)CTX3C的線性范圍是0.6-150 ng·L-1,相關(guān)系數(shù)是0.9948,而最低檢出限達(dá)到了0.09ng·L-1。本方法具有良好的穩(wěn)定性和重現(xiàn)性,而且能夠用于魚類樣品中魚肉素的定量檢測(cè)。第三章,基于納米金信號(hào)放大的膠束電動(dòng)毛細(xì)管色譜法在線檢測(cè)苯胺和對(duì)甲苯胺。本實(shí)驗(yàn)提出了一種膠束電動(dòng)毛細(xì)管色譜在線一次富集與納米金二次信號(hào)放大聯(lián)用的檢測(cè)方法,用于快速在線檢測(cè)苯胺和對(duì)甲苯胺。首先是在靜電作用下,將帶有正電荷的樣品聚集到帶有負(fù)電荷的膠團(tuán)表面,形成帶有正電荷的一次聚集體;然后在毛細(xì)管中引入帶有負(fù)電荷的納米金,同樣在分子間靜電作用下,帶有正電荷的膠團(tuán)聚集到納米金顆粒表面,達(dá)到檢測(cè)信號(hào)二次放大的目的。這種用于在線檢測(cè)苯胺和對(duì)甲苯胺的方法,具有靈敏度高、操作簡(jiǎn)單、穩(wěn)定性好等優(yōu)點(diǎn)。苯胺和對(duì)甲苯胺的最低檢出限分別達(dá)到了0.114×10-9 mol·L-1和2.656×10-9 mol·L-1。與毛細(xì)管區(qū)帶電泳以及單純的膠束電動(dòng)毛細(xì)管色譜法相比,本方法用于檢測(cè)苯胺和對(duì)甲苯的靈敏度分別提高了13421倍、6740倍和972倍、361倍。
[Abstract]:In this paper, the application of nanomaterials in the on-line enrichment of capillary electrophoresis was studied. Due to their good biocompatibility, large specific surface area, stable physical and chemical properties, etc. This paper is divided into three chapters: the first chapter mainly introduces the capillary electrophoresis technology. This paper discusses the development and application of capillary electrophoresis, focusing on several separation modes of capillary electrophoresis. Then, the development of capillary electrophoresis online enrichment technology is summarized. Secondly, the present situation of capillary electrophoresis research at home and abroad is prospected. Some innovations that play an important role in the development of capillary electrophoresis are introduced. Finally, the application of nanomaterials in capillary electrophoresis is introduced. Several functional nanomaterials with important properties and their roles in capillary electrophoresis separation are listed respectively. The detection of ciguatera venom by capillary electrophoresis based on external magnetic field and gold nanoparticles amplification is very sensitive and on line. In this chapter, a fast sensitive on-line method based on capillary electrophoresis separation and electrochemical immunoassay is proposed. A method for detecting CTX3C. In an immunological test of this sandwich sandwich structure, Using magnetic nanomaterials modified with CTX3C antibodies as probes, At the same time, horseradish peroxidase and CTX3C antibody were modified on the surface of gold nanoparticles as recognition factors. By setting an external magnetic field outside the capillary injection port, the on-line synthesis of immune complexes was realized. Compared with HPLC-MS and enzyme-linked immunosorbent assay (Elisa), This method, which uses nano-gold as multi-enzyme carrier and forms sandwich structure immune complex based on external magnetic field, has high sensitivity. The linear range of this method is 0.6-150 ng 路L ~ (-1), the correlation coefficient is 0.9948, and the minimum detection limit is 0.09 ng 路L ~ (-1). The method has good stability and reproducibility. And it can be used for quantitative determination of fish meat in fish samples. Micellar Electrokinetic Capillary Chromatography (MECC) based on nanoscale gold signal amplification was used to detect aniline and p-toluidine on line. In this paper, a new method for the on-line detection of aniline and p-toluidine by micellar electrokinetic capillary chromatography was proposed. It is used for rapid on-line detection of aniline and p-toluidine. Firstly, under electrostatic action, samples with positive charge are gathered on the surface of the micelle with negative charge to form a positive charge aggregate. Then the negatively charged nanocrystalline gold is introduced into the capillary, and the positively charged micelles gather on the surface of the nanoparticles under the same intermolecular electrostatic action. The method used for on-line detection of aniline and p-toluidine has high sensitivity and simple operation. The minimum detection limits of aniline and p-toluidine were 0.114 脳 10 ~ (-9) mol 路L ~ (-1) and 2.656 脳 10 ~ (-9) mol 路L ~ (-1), respectively, compared with capillary zone electrophoresis and micellar electrokinetic capillary chromatography. The sensitivity of this method for the determination of aniline and toluene was increased by 13421 times, 6740 times and 972 times, respectively.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O658.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Matthew S.MacLennan;Cai Tie;Kevin Kovalchik;Kerry M.Peru;Xinxiang Zhang;John V.Headley;David D.Y.Chen;;Potential of capillary electrophoresis mass spectrometry for the characterization and monitoring of amine-derivatized naphthenic acids from oil sands process-affected water[J];Journal of Environmental Sciences;2016年11期

2 劉鴻;姚捚;楊們?cè)?樊惠芝;;肽段固相等電聚焦結(jié)合液相色譜-質(zhì)譜聯(lián)用技術(shù)分析鼠肝蛋白質(zhì)[J];分析化學(xué);2014年02期

，

本文編號(hào)：1554615