本文關鍵詞： 毛細管電泳 膠束電動毛細管色譜 納米材料 西甲魚毒 苯胺 對甲苯胺　出處：《青島科技大學》2017年碩士論文　論文類型：學位論文

【摘要】：本文主要研究了納米材料在毛細管電泳在線富集方法上的應用,由于納米材料具有生物相容性好、比表面積大、物理化學性質(zhì)穩(wěn)定等特點,于毛細管在線富集方法上具有顯著作用。通過對納米材料的應用,富集效果顯著提高。本文共分為三個章節(jié):第一章,主要對毛細管電泳技術(shù)進行了介紹。首先,討論了毛細管電泳的發(fā)展及應用,重點涉及毛細管電泳的幾種分離模式;然后,對毛細管電泳在線富集技術(shù)的發(fā)展進行了概括;其次,展望國內(nèi)外毛細管電泳研究現(xiàn)狀,對一些在毛細管電泳發(fā)展過程中具有重要作用的創(chuàng)新進行了介紹;最后,介紹了納米材料在毛細管電泳上的應用,分別列舉了幾種具有重要特性的功能性納米材料以及在毛細管電泳分離方法上所起到的作用。第二章,基于外部磁場和納米金放大的毛細管電泳免疫分析超靈敏在線快速檢測雪卡魚毒。本章節(jié)提出了一種基于毛細管電泳分離與電化學免疫分析聯(lián)用的快速靈敏在線檢測CTX3C的方法。在這種三明治夾心結(jié)構(gòu)的的免疫實驗中,將修飾有CTX3C抗體的磁性納米材料作為探針,同時將辣根過氧化物酶以及CTX3C抗體修飾到納米金顆粒表面上作為識別因子。通過在毛細管進樣口外部設置一個外部磁場使免疫復合物在線合成得以實現(xiàn)。與傳統(tǒng)的高效液相色譜-質(zhì)譜以及酶聯(lián)免疫反應方法相比,這種采用納米金作為多酶載體、基于外部磁場在線形成三明治結(jié)構(gòu)的免疫復合物的方法具有靈敏度高、分析時間短等特點。本方法檢測CTX3C的線性范圍是0.6-150 ng·L-1,相關系數(shù)是0.9948,而最低檢出限達到了0.09ng·L-1。本方法具有良好的穩(wěn)定性和重現(xiàn)性,而且能夠用于魚類樣品中魚肉素的定量檢測。第三章,基于納米金信號放大的膠束電動毛細管色譜法在線檢測苯胺和對甲苯胺。本實驗提出了一種膠束電動毛細管色譜在線一次富集與納米金二次信號放大聯(lián)用的檢測方法,用于快速在線檢測苯胺和對甲苯胺。首先是在靜電作用下,將帶有正電荷的樣品聚集到帶有負電荷的膠團表面,形成帶有正電荷的一次聚集體;然后在毛細管中引入帶有負電荷的納米金,同樣在分子間靜電作用下,帶有正電荷的膠團聚集到納米金顆粒表面,達到檢測信號二次放大的目的。這種用于在線檢測苯胺和對甲苯胺的方法,具有靈敏度高、操作簡單、穩(wěn)定性好等優(yōu)點。苯胺和對甲苯胺的最低檢出限分別達到了0.114×10-9 mol·L-1和2.656×10-9 mol·L-1。與毛細管區(qū)帶電泳以及單純的膠束電動毛細管色譜法相比,本方法用于檢測苯胺和對甲苯的靈敏度分別提高了13421倍、6740倍和972倍、361倍。
[Abstract]:In this paper, the application of nanomaterials in the on-line enrichment of capillary electrophoresis was studied. Due to their good biocompatibility, large specific surface area, stable physical and chemical properties, etc. This paper is divided into three chapters: the first chapter mainly introduces the capillary electrophoresis technology. This paper discusses the development and application of capillary electrophoresis, focusing on several separation modes of capillary electrophoresis. Then, the development of capillary electrophoresis online enrichment technology is summarized. Secondly, the present situation of capillary electrophoresis research at home and abroad is prospected. Some innovations that play an important role in the development of capillary electrophoresis are introduced. Finally, the application of nanomaterials in capillary electrophoresis is introduced. Several functional nanomaterials with important properties and their roles in capillary electrophoresis separation are listed respectively. The detection of ciguatera venom by capillary electrophoresis based on external magnetic field and gold nanoparticles amplification is very sensitive and on line. In this chapter, a fast sensitive on-line method based on capillary electrophoresis separation and electrochemical immunoassay is proposed. A method for detecting CTX3C. In an immunological test of this sandwich sandwich structure, Using magnetic nanomaterials modified with CTX3C antibodies as probes, At the same time, horseradish peroxidase and CTX3C antibody were modified on the surface of gold nanoparticles as recognition factors. By setting an external magnetic field outside the capillary injection port, the on-line synthesis of immune complexes was realized. Compared with HPLC-MS and enzyme-linked immunosorbent assay (Elisa), This method, which uses nano-gold as multi-enzyme carrier and forms sandwich structure immune complex based on external magnetic field, has high sensitivity. The linear range of this method is 0.6-150 ng 路L ~ (-1), the correlation coefficient is 0.9948, and the minimum detection limit is 0.09 ng 路L ~ (-1). The method has good stability and reproducibility. And it can be used for quantitative determination of fish meat in fish samples. Micellar Electrokinetic Capillary Chromatography (MECC) based on nanoscale gold signal amplification was used to detect aniline and p-toluidine on line. In this paper, a new method for the on-line detection of aniline and p-toluidine by micellar electrokinetic capillary chromatography was proposed. It is used for rapid on-line detection of aniline and p-toluidine. Firstly, under electrostatic action, samples with positive charge are gathered on the surface of the micelle with negative charge to form a positive charge aggregate. Then the negatively charged nanocrystalline gold is introduced into the capillary, and the positively charged micelles gather on the surface of the nanoparticles under the same intermolecular electrostatic action. The method used for on-line detection of aniline and p-toluidine has high sensitivity and simple operation. The minimum detection limits of aniline and p-toluidine were 0.114 脳 10 ~ (-9) mol 路L ~ (-1) and 2.656 脳 10 ~ (-9) mol 路L ~ (-1), respectively, compared with capillary zone electrophoresis and micellar electrokinetic capillary chromatography. The sensitivity of this method for the determination of aniline and toluene was increased by 13421 times, 6740 times and 972 times, respectively.
【學位授予單位】：青島科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O658.9

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