本文關(guān)鍵詞： 離子交換色譜 聚合物接枝 反離子 吸附平衡 動(dòng)力學(xué) 動(dòng)態(tài)結(jié)合容量 線性梯度洗脫　出處：《化工學(xué)報(bào)》2017年11期 　論文類型：期刊論文

【摘要】：前期研究表明部分電荷中和的聚乙烯亞胺接枝瓊脂糖介質(zhì)FF-PEI-R440具有較高靜態(tài)吸附容量和快速傳質(zhì)速率。本文選取硫氰酸根離子、氯離子、磷酸氫根離子以及硫酸根離子這4種反離子,在離子強(qiáng)度為0.03、0.06mol·L~(-1)下,研究其對(duì)FF-PEI-R440吸附與洗脫行為的影響。結(jié)果表明:在兩種離子強(qiáng)度下,飽和吸附容量的增加順序均為SCN-SO_4~(2-)HPO42-Cl~-;在低離子強(qiáng)度下,除SCN-外,反離子種類對(duì)傳質(zhì)速率和動(dòng)態(tài)結(jié)合容量沒有顯著影響;在高離子強(qiáng)度下,傳質(zhì)速率的增加順序?yàn)镾CN-SO_4~(2-)≈Cl~-HPO42-,而動(dòng)態(tài)結(jié)合容量的增加順序與飽和吸附容量序列一致;兩種離子強(qiáng)度下Cl~-均保持最高的動(dòng)態(tài)結(jié)合容量;反離子種類對(duì)蛋白質(zhì)洗脫行為沒有顯著影響。上述結(jié)果說明反離子主要影響FF-PEI-R440的吸附性能,而不影響洗脫,且流動(dòng)相中選擇Cl~-為反離子最利于FF-PEI-R440的實(shí)際柱色譜操作。
[Abstract]:Previous studies have shown that the partially charge neutralized polyvinyleneimine grafted agarose medium FF-PEI-R440 has high static adsorption capacity and rapid mass transfer rate. In this paper, thiocyanate ion, chloride ion and thiocyanate ion are selected. The effects of hydrogen phosphate ion and sulfate ion on the adsorption and elution behavior of FF-PEI-R440 were studied at the ionic strength of 0.03 ~ 0.06 mol 路L ~ (-1). The increasing order of saturated adsorption capacity is SCN-SO4 / 2-HPO42-Cl-C, and at low ion intensity, there is no significant effect on mass transfer rate and dynamic binding capacity except for SCN-; at high ion intensity, the mass transfer rate and dynamic binding capacity are not significantly affected by the type of counterions. The increasing order of mass transfer rate is SCN-SO4T _ 2) 鈮，

本文編號(hào)：1549221