本文關鍵詞： 野生沉香 鑒別方法 高效液相色譜 指紋圖譜 共有特征峰　出處：《林業(yè)科學》2017年09期 　論文類型：期刊論文

【摘要】：【目的】建立有效、準確、科學的野生沉香鑒別技術,為沉香的鑒定和品質評價體系建立提供參考依據(jù)�！痉椒ā坎捎么既苄越鑫锖俊@色反應、薄層色譜分析(TLC)和高效液相色譜(HPLC)方法,對10批次野生沉香進行鑒別�！窘Y果】10批次野生沉香的醇溶性浸出物含量整體較高,明顯大于《中華人民共和國藥典》(2015年版)規(guī)定的10.0%;野生沉香的顯色反應除呈現(xiàn)人工沉香的櫻紅色或淺櫻紅外,還出現(xiàn)紫堇色、淺紅色和淺紫色在內的多樣性顯色,可輔助鑒別野生沉香;薄層色譜操作簡單,節(jié)省材料,且野生沉香樣品在薄層鑒別中呈現(xiàn)清晰的熒光斑點和較好的分離效果,對于大部分野生沉香樣品可通過薄層色譜進行基礎鑒別;通過相似度評價、峰面積比較,建立10批次野生沉香的HPLC指紋圖譜,并確定9個野生沉香共有特征峰,確定野生和人工沉香HPLC特征指紋圖譜的差異性�！窘Y論】基于各方法的優(yōu)勢及精準程度,本文提出在顯微鑒別的基礎上,通過醇溶性浸出物含量、顯色反應、薄層色譜分析和HPLC的指紋圖譜及特征峰鑒別野生沉香,可為沉香品質評價體系建立提供參考。
[Abstract]:[objective] to establish an effective, accurate and scientific identification technique for wild Amorpha sinensis, and to provide a reference for the identification and quality evaluation system. [methods] Alcohol-soluble extracts were used for color reaction. TLC (TLC) and HPLC (High performance liquid Chromatography) methods were used to identify 10 batches of wild Acanthia sinensis. [results] the alcohol-soluble extracts of 10 batches of wild Acanthia sinensis were higher in whole. It is obviously larger than the 10. 0 specified in the Pharmacopoeia of the people's Republic of China (2015 edition). The color reaction of wild Amomum sinensis is not only the fuchsia red or light cherry infrared, but also the color of Corydalis, light red and light purple. TLC was easy to operate and save materials, and the samples of wild Amorpha showed clear fluorescent spots and good separation effect in TLC identification. For most of the samples, TLC can be used for basic identification, and through similarity evaluation, peak area comparison, the HPLC fingerprint of 10 batches of wild Acanthia sinensis was established, and 9 common characteristic peaks were determined. [conclusion] based on the advantages and accuracy of each method, this paper proposed that the content of alcohol soluble extracts and color reaction should be used on the basis of microscopic identification. TLC, fingerprint and characteristic peak of HPLC can be used to identify wild Amorpha sinensis, which can provide reference for the establishment of quality evaluation system.
【作者單位】： 中國林業(yè)科學研究院林業(yè)新技術研究所;中國林業(yè)科學研究院木材工業(yè)研究所;中國熱帶農業(yè)科學院熱帶生物技術研究所;
【基金】：中央級公益性科研院所基本科研業(yè)務費專項資金(CAFINT2015C05) 林業(yè)標準制修訂項目《沉香》(2014-LY-071)
【分類號】：O657.7;TQ654.2
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本文編號：1499425