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  本文關鍵詞： 廣豆根 黃酮類 黑升麻 皂苷類 分離鑒定　出處：《西南科技大學》2016年碩士論文　論文類型：學位論文

【摘要】：廣豆根及黑升麻具有抗腫瘤、抗炎等藥理功效,是重要的傳統(tǒng)中藥材。廣豆根所含的黃酮類成分具有較好的抗?jié)兓钚院秃谏樗脑碥粘煞志哂休^好的緩解更年期作用。本文對廣豆根的黃酮類成分和黑升麻皂苷成分進行研究,為藥理活性和構(gòu)效關系奠定基礎。采用硅膠柱色譜、葡聚糖凝膠色譜Sephadex LH-20、反相柱色譜及高效液相色譜(High Performance Liquid Chromatography,HPLC)等色譜技術,并結(jié)合結(jié)晶與重結(jié)晶等分離手段,對廣豆根進行分離純化。采用質(zhì)譜(Mass Spectrometry,MS)、核磁共振波譜(Nuclear Magnetic Resonance Spectroscopy,NMR)、紫外光譜(Ultraviolet Absorption Spectroscopy,UV)等現(xiàn)代波譜學技術,對分離到的化合物進行結(jié)構(gòu)鑒定。采用色譜技術,從廣豆根的二氯甲烷部分,分離到11個化合物。經(jīng)波譜學技術,鑒定出9個黃酮類化合物:廣豆根素(1,1.300 g)、多花胡枝子素A3(2,0.810 g)、L-高麗槐素(3,0.325 g)、光甘草酚(4,0.056 g)、廣豆根酮(7,0.051 g)、山豆根色烯素(8,0.750 g)、Lupinifolin(9,0.015 g)、5-Hydroxysophoranone(10,0.18 g),同時還得到3個非黃酮類化合物:β-谷甾醇(5,0.098 g)羽扇豆醇(6,0.043 g)和大豆皂醇B(11,0.010 g)�；衔�2為首次從該屬植物中分離得到,化合物10為首次從該植物中分離得到。采用反復柱色譜和重結(jié)晶等方法對黑升麻的乙酸乙酯部位進行分離純化,從黑升麻中分離得到黑升麻苷(0.208 g)和27位脫氧黑升麻苷(0.017 g),純度分別為98.2%和98.3%,產(chǎn)率分別是0.020%和0.001%。
[Abstract]:Gangdou root and black cohosh have anti-tumor, anti-inflammatory and other pharmacological effects. It is an important traditional Chinese medicinal material. The flavonoids contained in the root of Guangdou have better anti-ulcer activity and the saponins contained in the black cohosh have a better effect on relieving climacteric. The components of saponins were studied. For the basis of pharmacological activity and structure-activity relationship, silica gel column chromatography and dextran gel chromatography (Sephadex LH-20) were used. Reversed phase column chromatography (RPC) and high performance liquid chromatography (HPLC) with high Performance Liquid chromatographyHPLC. In combination with crystallization and recrystallization, the root was separated and purified by mass spectrometric mass spectrometry (MS). Nuclear Magnetic Resonance spectroscopy (NMRs). Ultraviolet Absorption Spectroscopy (UV) and other modern spectroscopic techniques. 11 compounds were isolated from the dichloromethane part of Gangdou root by chromatographic technique, and 9 flavonoids were identified by spectroscopic technique. 1.300 g / g, 0.300 g / g, 0.810 g / L, 0.325 g / L, 0.056 g / L, 0.056 g / L, 7g / L, respectively. 0. 051 g / g, Lupinifolin9 (0. 015 g). 5-Hydroxysophoranoneum 100.18 g, and three non-flavonoids, 尾 -sitosterol 50.098 g) were also obtained. Compound 2 was isolated from this genus for the first time. Compound 10 was isolated from the plant for the first time. Repeated column chromatography and recrystallization were used to isolate and purify ethyl acetate from black cohosh. The purity was 98.2% and 98.3%, respectively. The yields were 0.020% and 0.001, respectively.
【學位授予單位】：西南科技大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R284;O657.7

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本文編號：1489139