本文關(guān)鍵詞： 糖聚合物 傒酰亞胺 光聲成像 蛋白質(zhì)　出處：《高分子學(xué)報(bào)》2017年12期 　論文類型：期刊論文

【摘要】：設(shè)計(jì)合成了具備良好的近紅外吸收性能的傒酰亞胺大分子引發(fā)劑,并通過原子轉(zhuǎn)移自由基聚合(ATRP)和后修飾方法制備了重復(fù)單元分別為一位羥基葡萄糖(1-Glc)和六位羥基葡萄糖(6-Glc)的糖聚合物.該聚合物在水溶液中能形成傒酰亞胺為核,糖聚合物為殼的納米聚集體.通過動(dòng)態(tài)光散射(DLS)、透射電子顯微鏡(TEM)和紫外-可見吸收光譜(UV-Vis)對(duì)聚合物在水溶液中的形貌、大小以及光學(xué)性能進(jìn)行了分析表征,聚合物在650~750 nm有較好的近紅外吸收特性;通過光聲儀測(cè)試了聚合物的光聲性能,結(jié)果表明傒酰亞胺糖聚合物具有較強(qiáng)的光聲信號(hào);為研究葡萄糖異構(gòu)化對(duì)糖聚合物蛋白識(shí)別功能的影響,采用多種表征手段如濁度法、動(dòng)態(tài)光散射研究了聚合物和凝集素半刀豆蛋白(concanavalin A,Con A)的結(jié)合.發(fā)現(xiàn)隨著Con A凝集素的加入,PBI-1-Glc溶液的濁度和粒徑有著明顯的增加,而PBI-6-Glc則幾乎沒有變化;此外,還利用光聲成像技術(shù)研究了糖聚合物與Con A之間的相互作用,Con A凝集素可使PBI-1-Glc聚合物的光聲信號(hào)明顯增加,此結(jié)果表明光聲技術(shù)也可以有效地識(shí)別2種聚合物與凝集素之間的相互作用.實(shí)驗(yàn)結(jié)果顯示,PBI-1-Glc聚合物與凝集素Con A有強(qiáng)的相互作用,而PBI-6-Glc聚合物則沒有,這證實(shí)了單糖異構(gòu)化在影響其與凝集素的特異性識(shí)別方面具有一定普適性.
[Abstract]:An imide macromolecular initiator with good near infrared absorption property was designed and synthesized. The repeat units were prepared by atom transfer radical polymerization (ATRP) and post modification, respectively. The repeat units were one hydroxyl glucose 1-Glc and six hydroxyl glucose 6-Glc, respectively. The polymer can form imide as core in aqueous solution. The morphology of the polymer in aqueous solution was investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and UV-Vis-UV absorption spectroscopy (UV-Vis). The size and optical properties of the polymer were analyzed and characterized. The polymer has good near infrared absorption at 650 ~ 750 nm. The photoacoustic properties of the polymer were measured by photoacoustic instrument. The results show that the polymer has a strong photoacoustic signal. In order to study the effect of glucose isomerization on the recognition function of sugar polymer protein, many characterization methods such as turbidimetric method were used. Dynamic light scattering (DLS) was used to study the binding of polymer to concanavalin Aconcanavalin Con A. It was found that with the addition of Con A agglutinin. The turbidity and particle size of PBI-1-Glc solution increased obviously, but PBI-6-Glc had little change. In addition, the photoacoustic imaging technique was used to study the interaction between sugar polymer and Con A. The lectin of Con A can increase the photoacoustic signal of PBI-1-Glc polymer. The results show that the photoacoustic technique can also effectively identify the interaction between two kinds of polymers and lectins. The experimental results show that PBI-1-Glc polymer has strong interaction with lectin Con A. However, PBI-6-Glc polymers do not, which proves that monosaccharide isomerization has a certain universality in affecting its specific recognition with lectins.
【作者單位】： 南京工業(yè)大學(xué)江蘇省柔性電子重點(diǎn)實(shí)驗(yàn)室先進(jìn)材料研究院江蘇先進(jìn)生物與化學(xué)制造協(xié)同創(chuàng)新中心;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃,項(xiàng)目號(hào)2015CB932200) 國家自然科學(xué)基金(基金號(hào)21574064,61136003)資助項(xiàng)目
【分類號(hào)】：O631
【正文快照】： **通訊聯(lián)系人,E-mail:wei-huang@njtech.edu.cn糖類化合物作為維持生命與健康的營養(yǎng)物質(zhì)之一,不僅為生物體提供主要能源,而且還具備多種生物功能,其中糖-蛋白之間的特異性識(shí)別在細(xì)胞識(shí)別、細(xì)胞黏附、病菌感染、免疫反應(yīng)等過程中發(fā)揮著重要的作用[1~8].糖異構(gòu)現(xiàn)象廣泛存在于糖，

本文編號(hào)：1488985