【摘要】：本文研究了不同濃度草甘膦與殺蟲單對60-70代泥鰍鰭二倍體細胞系(DIMF)和泥鰍鰭三倍體細胞系(TRMF)的毒性作用。采用噻唑藍(MTT)法測得草甘膦對DIMF與TRMF 24h半致死濃度分別為333.79mg/L,384.91mg/L,殺蟲單對DIMF與TRMF 24h半致死濃度分別為119.73mg/L,146.26mg/L。DIMF對草甘膦和殺蟲單的敏感性明顯高于TRMF。經(jīng)草甘膦與殺蟲單處理的活體細胞均表現(xiàn)為細胞拉長,空泡化和脫落并游離于培養(yǎng)基表面的現(xiàn)象。酶活力測定兩種細胞系的結果顯示:草甘膦濃度為0~300mg/L時,超氧化物歧化酶(SOD)和谷胱甘肽S轉(zhuǎn)移酶(GST)活力隨著濃度的增加而增加,隨后逐漸降低;乙酰膽堿酯酶(ACh E)活力與草甘膦濃度呈負相關。殺蟲單濃度為0~100mg/L時,SOD和GST活力隨著濃度的增加而增加,100~200mg/L濃度組酶活力逐漸降低;ACh E活力與殺蟲單濃度呈負相關。并且三種酶活力三倍體均高于二倍體。微核試驗結果顯示:100mg/L草甘膦與50mg/L殺蟲單即能使細胞核產(chǎn)生損傷而形成微核,微核率隨著草甘膦與殺蟲單濃度增加而增加,草甘膦濃度為500mg/L和殺蟲單濃度為200mg/L時微核率最大,草甘膦處理組DIMF與TRMF微核率均為4.3‰,殺蟲單處理組DIMF與TRMF微核率分別為3.3‰和3.7‰。電鏡觀察結果是對照組DIMF和TRMF超微結構無明顯差異。經(jīng)草甘膦與殺蟲單處理后,DIMF和TRMF病理變化情況相似:染色質(zhì)凝集趨邊,細胞核分解成多個;線粒體、核糖體與內(nèi)質(zhì)網(wǎng)等細胞器數(shù)量減少,細胞內(nèi)出現(xiàn)空泡,出現(xiàn)凋亡小體,表現(xiàn)出凋亡的特征。本文不僅研究了草甘膦與殺蟲單毒性的作用機制和不同倍性細胞系之間的差異,而且為建立草甘膦與殺蟲單農(nóng)藥體外監(jiān)控體系打下基礎。
[Abstract]:In this paper, the toxicity of glyphosate and diploid of 60 渭 m 70 generation Loach fin diploid cell line (DIMF) and Loach fin triploid cell line (TRMF) was studied in different concentrations of glyphosate and insecticida. The 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L for DIMF and TRMF, respectively, determined by thiazole blue (MTT) method, and the 24-hour lethal concentrations of glyphosate to DIMF and TRMF were 333.79 mg / L, 384.91 mg / L and 119.73 mg / L, respectively. The sensitivity of 146.26mg/L.DIMF to glyphosate and insecticidal list was significantly higher than that of TRMF.. The cells treated with glyphosate and insecticidal single showed the phenomenon of cell lengthening, vacuolization and exfoliation and dissociating on the surface of the culture medium. The results of enzyme activity assay showed that when the concentration of glyphosate was 0~300mg/L, the activities of superoxide dismutase (SOD) and glutathione S transferase (GST) increased with the increase of the concentration, and then decreased gradually. Acetylcholinesterase (ACh E) activity was negatively correlated with glyphosate concentration. When the concentration of 0~100mg/L was 0~100mg/L, the activities of SOD and GST increased with the increase of the concentration, and the decrease of the activity of; ACh E in the 100~200mg/L concentration group was negatively correlated with the concentration of the insecticidal single. The activity of the three enzymes in triploid was higher than that in diploid. The results of micronucleus test showed that 100mg/L glyphosate and 50mg/L were able to damage the nucleus and form micronucleus, and the micronucleus rate increased with the increase of glyphosate and insecticidal single concentration. When the concentrations of glyphosate were 500mg/L and 200mg/L, the micronucleus rates of DIMF and TRMF were 4.3 鈥，

本文編號：2443045