【摘要】：通過淬滅細(xì)菌的群體感應(yīng)系統(tǒng)來抑制生物膜形成、防止膜生物污染的方法近年來受到廣泛關(guān)注.本實(shí)驗(yàn)從實(shí)際運(yùn)行污水處理廠活性污泥中分離出5株具有群體感應(yīng)淬滅功能的菌株,其中菌株HG10對信號(hào)分子N-乙酰高絲氨酸環(huán)內(nèi)酯(C6-HSL)分解能力最強(qiáng).經(jīng)16S rRNA基因序列比對,初步鑒定為蠟樣芽孢桿菌(Bacillus cereus).用海藻酸鈉將菌株HG10進(jìn)行包埋固定,以探究其在膜過濾系統(tǒng)中對膜污染防治的效果.結(jié)果表明,經(jīng)過8 d培養(yǎng),添加細(xì)菌包埋珠(SA-HG10)的實(shí)驗(yàn)組B中膜通量為181.29 L·(m~2·h)~(-1),未投加包埋珠的對照組A膜通量為110.64 L·(m~2·h)~(-1),B組膜通量比A組高出63.86%;對微濾膜片上生物膜中EPS含量測定表明,實(shí)驗(yàn)組B中EPS多糖和蛋白質(zhì)含量較對照組A分別減少了29%和48%,疏水性蛋白質(zhì)含量的大量減少是造成膜污染減弱的主要原因;膜表面胞外聚合物(EPS)總含量減少了43%,表明投放SA-HG10細(xì)菌包埋珠對過濾膜片上生物膜形成具有明顯抑制作用,改善了膜過濾性能.
[Abstract]:The methods of inhibiting biofilm formation and preventing membrane biofilm fouling through colony sensing system of quenched bacteria have been paid more and more attention in recent years. In this experiment, five strains with colony induction quenching function were isolated from activated sludge of practical sewage treatment plant. Among them, strain HG10 had the strongest ability to decompose signal molecule N- acetyl homoserine cyclic lactone (C6-HSL). Bacillus cereus (Bacillus cereus). Was identified as Bacillus cereus by 16s rRNA gene sequence alignment. The strain HG10 was immobilized with sodium alginate to investigate its effect on membrane fouling in membrane filtration system. The results showed that after 8 days of culture, the membrane flux of experimental group B was 181.29 L (m ~ (2 h) ~ (-1), that of control group was 110.64 L (m ~ (2 h) ~ (-1) ~ (-1), the flux of group B was 63.86 higher than that of group A, and the content of EPS in biofilm was determined. The contents of EPS polysaccharides and proteins in experimental group B were decreased by 29% and 48%, respectively, compared with control group A. the decrease of hydrophobic protein content was the main reason for the decrease of membrane fouling. The total content of extracellular polymer (EPS) on the membrane surface was reduced by 43%, which indicated that the implantation of SA-HG10 bacterial entrapment beads could significantly inhibit the formation of biofilm on the membrane and improve the filtration performance of the membrane.
【作者單位】： 北京大學(xué)深圳研究生院環(huán)境與能源學(xué)院聚硅酸鹽復(fù)合環(huán)保材料工程實(shí)驗(yàn)室;
【基金】：深圳市科技計(jì)劃項(xiàng)目(JCYJ20150626110817181,JCYJ20150806112326712,CXZZ20151117141320317)
【分類號(hào)】：X703;X172

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本文編號(hào)：2192506