本文選題：苯系物 + 仿刺參��； 參考：《生態(tài)毒理學(xué)報(bào)》2016年06期

【摘要】：為了分析苯系物(BTEXs)聯(lián)合暴露后仿刺參(Apostichopus japonicus)管足轉(zhuǎn)錄組差異表達(dá)基因,采用Illumina Hi SeqTM2000測(cè)序技術(shù),對(duì)1.0 mg·L~(-1)苯系物(B)聯(lián)合暴露12 h后和對(duì)照組(C)的仿刺參管足組織分別進(jìn)行轉(zhuǎn)錄組測(cè)序。經(jīng)Trinity軟件進(jìn)行de novo組裝,獲得了145 675條unigenes。利用公共數(shù)據(jù)庫(kù)進(jìn)行同源比對(duì),共注釋了35 330條unigenes。對(duì)比分析苯系物聯(lián)合暴露組和對(duì)照組的轉(zhuǎn)錄組測(cè)序結(jié)果,獲得了2 418個(gè)差異表達(dá)基因(DEGs)(|Log2Fold changes|≥1且FDR≤0.001),其中,上調(diào)表達(dá)和下調(diào)表達(dá)基因分別為1 049和1 369個(gè)。GOseq分析結(jié)果顯示,158個(gè)DEGs顯著富集在149個(gè)GO terms中,包括103個(gè)生物學(xué)過程、17個(gè)細(xì)胞組分和29個(gè)分子功能(P0.05);KEGG代謝通路分析結(jié)果顯示994個(gè)差異基因映射到268條代謝通路,這些差異表達(dá)基因參與的生理過程與其他生物的同源基因參與的信號(hào)傳導(dǎo)、癌癥、外源性化合物的生物降解代謝等過程相類似。上述結(jié)果為在轉(zhuǎn)錄組水平篩選苯系物的生物標(biāo)志物,解析苯系物對(duì)仿刺參毒性作用的分子機(jī)制提供了科學(xué)參考。
[Abstract]:In order to analyze the differentially expressed genes of (Apostichopus japonicus) tube foot transcripts after combined exposure to benzene series (BTEXs), Illumina Hi SeqTM2000 sequencing technique was used to analyze the transcriptional sequence of 1. 0 mg L ~ (-1) benzene series (B) combined with exposure for 12 h and that of the control group (C). A total of 145,675 unigenes were obtained from de novo assembly by Trinity software. A total of 35,330 unigeneses were annotated by using common database for homologous alignment. A total of 2 418 differentially expressed genes (decs 鈮，

本文編號(hào)：2052113