本文選題：甲醛 + 甲醇��； 參考：《環(huán)境科學研究》2016年12期

【摘要】：為了探究甲醛(Formaldehyde,FA)對造血組織的毒性,分析甲醛的毒性是否與中間代謝產(chǎn)物——甲醇(Methanol,MeOH)的毒性一致,以72只昆明小鼠為受試動物,隨機分為6組〔1對照組,僅灌服生理鹽水;2FA40組,單獨灌服甲醛(FA),w(FA)為40 mg/kg;3 MeOH40組,單獨灌服甲醇(MeOH),w(MeOH)為40 mg/kg;4FA_(10)+MeOH30組,同時灌服甲醛和甲醇,w(FA)為10mg/kg、w(MeOH)為30 mg/kg;5FA_(20)+MeOH20組,同時灌服甲醛和甲醇,w(FA)為20 mg/kg、w(MeOH)為20 mg/kg;6FA_(30)+MeOH10組〕,同時灌服甲醛和甲醇,w(FA)為30 mg/kg、w(MeOH)為10 mg/kg,連續(xù)灌胃7 d,檢測小鼠的肝臟氧化損傷程度、血液和骨髓內(nèi)甲醛含量以及甲醛脫氫酶的相對表達量,比較甲醇和甲醛毒性的差異性.結(jié)果表明:與對照組相比,各染毒組小鼠肝臟內(nèi)ROS(reactive oxygen,活性氧)含量極顯著增加(P0.01),FA_(30)+MeOH10組的ROS含量顯著上升(P0.05);肝臟內(nèi)MDA(malondialdehyde,丙二醛)含量極顯著下降(P0.01),GSH(glutathione,谷胱甘肽)含量極顯著升高(P0.01);在甲醛和甲醇聯(lián)合染毒組中,隨著甲醛含量的增加和甲醇含量的減少,氧化損傷程度呈上升趨勢.AHMT(4-amino-3-hydrazine-5-mercapto-1,2,4-triazole)法測定結(jié)果顯示,各染毒組小鼠血液和骨髓中甲醛含量與對照組相比均無顯著差異;RT-PCR法測定結(jié)果顯示,各染毒組小鼠中甲醛脫氫酶的相對表達量較對照組顯著增加;FA40組與MeOH40組甲醛脫氫酶的相對表達量差異顯著(P0.01);與FA_(30)+MeOH10組相比,FA_(10)+MeOH30組中甲醛脫氫酶的相對表達量極顯著增加(P0.01),甲醛和甲醇聯(lián)合染毒組中甲醛脫氫酶的相對表達量隨著甲醛含量的增加和甲醇含量的減少呈上升趨勢.研究顯示,甲醛和甲醇的毒性不一致,甲醛對造血組織的毒性可能存在其他方式.
[Abstract]:In order to investigate the toxicity of formaldehyde (FA) to hematopoietic tissue, and analyze whether the toxicity of formaldehyde is consistent with that of Methanolol MeOH, 72 Kunming mice were randomly divided into 6 groups (control group). Normal saline 2FA40 group, formalin (FA) w (FA) 40 mg / kg 3MeOH40 group, methanol (MeOH) 4FAH30 group (MeOH) 40 mg / kg ~ (10) FAH _ (10) MeOH30, formaldehyde and methanol w (FA) 10 mg 路kg ~ (-1) F _ (20) MeOH _ (20) MeOH _ (20) MeOH20 group, formaldehyde and methanol w (FA) 20 mg / kg 路kg ~ (-1) w (MeOH) = 20 mg / kg 路kg ~ (6) FAF _ (30) MeOH _ (10). At the same time, the concentrations of formaldehyde and methanol were 30 mg / kg 路kg ~ (-1) (MeOH) = 10 mg / kg for 7 days. The degree of liver oxidative damage, the formaldehyde content in blood and bone marrow, and the relative expression of formaldehyde dehydrogenase in blood and bone marrow were measured, and the difference of toxicity between methanol and formaldehyde was compared. The results showed that: compared with the control group, The content of Ros (reactive oxygen, in the liver of each exposure group was significantly increased (P0.01) and the content of malondialdehyde (MDA) in the liver was significantly decreased (P0.01), and the content of glutathione was significantly increased (P0.01) in the combined group of formaldehyde and methanol (P0.01), while the content of Ros in the liver increased significantly (P0.01), and the content of malondialdehyde (malondialdehyde) decreased significantly (P0.01) in the combined group of formaldehyde and methanol (P0.01), while in the combined group of formaldehyde and methanol, the content of Ros in the liver increased significantly (P0.01), and the content of glutathione in the liver increased significantly. With the increase of formaldehyde content and the decrease of methanol content, the degree of oxidative damage showed an increasing trend. AHMT (4-amino-3-hydrazine-5-mercapto-1mercapto-1titriazole) method showed that there was no significant difference in formaldehyde content in blood and bone marrow of mice in each group compared with the control group. The relative expression of formaldehyde dehydrogenase in mice of each exposure group was significantly higher than that in control group (P0.01), and the relative expression of formaldehyde dehydrogenase in FA10 MeOH30 group was extremely significant compared with FA30 MeOH10 group (P0.01). The relative expression of formaldehyde dehydrogenase increased with the increase of formaldehyde content and the decrease of methanol content. Studies have shown that the toxicity of formaldehyde to hematopoietic tissue is not consistent with that of methanol, and that there may be other ways of toxicity of formaldehyde to hematopoietic tissue.
【作者單位】： 華中師范大學生命科學學院;
【基金】：國家自然科學基金重點項目(51136002)
【分類號】：X171.5

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