本文關鍵詞：鹽度對SBR和SBBR工藝短程硝化反硝化的影響　出處：《中國海洋大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 鹽度 SBR SBBR 短程硝化反硝化 胞外聚合物

【摘要】：本論文利用序批式活性污泥反應器(SBR)和序批式生物膜反應器(SBBR),研究了鹽度變化對污染物去除和短程硝化反硝化穩(wěn)定性的影響。(1)用全程硝化污泥為接種污泥,在鹽度0.6%條件下控制供氣量(360 mL/min)和反應溫度(30℃),經(jīng)3個月啟動時間兩個反應器內(nèi)均實現(xiàn)了高效短程硝化反硝化。進水鹽度從1.0%逐步升高到5.0%時,鹽度的提高未對兩個反應器內(nèi)COD的去除產(chǎn)生明顯抑制作用。進水鹽度在1.0%-4.5%范圍時,兩個反應器出水NH4+-N、NO2--N和N03--N分別小于1.0,0.5,0.5 mg/L,鹽度的提高未對兩個反應器的硝化過程和反硝化過程產(chǎn)生抑制作用；在此鹽度范圍內(nèi),SBR中亞硝酸鹽積累率(NAR)均在97.00%以上,SBBR中NAR均在95.00%以上。進水鹽度提高到5.0%時,SBR和SBBR分別運行9d和7d后,NH4+-N的氧化過程幾乎被完全抑制,短程硝化被徹底破壞。(2)進水鹽度從0.6%逐步升高到5.0%時,SBR和SBBR中污泥容積指數(shù)(SVI)隨鹽度的增加先增加后降低。鹽度在1.0%-3.0%范圍時,SBR活性污泥、SBBR中懸浮污泥和生物膜的微生物活性比耗氧速率(SOUR)均隨鹽度的提高逐漸降低。鹽度在1.0%-5.0%范圍時,三種來源微生物的比氨氧化速率(SAOR)、比亞硝酸鹽還原速率(SNIRR)和比硝酸鹽還原速率(SNARR)均隨鹽度的增加出現(xiàn)不同程度的下降。鹽度增加對三種來源微生物的SAOR抑制程度均大于對SNIRR和SNARR的抑制程度,說明NO2--N和N03--N還原菌相比硝化細菌具有更強的耐鹽能力。此鹽度范圍內(nèi),三種來源微生物的SNIOR均未檢測出,鹽度為1.0%-3.0%范圍時,未檢測出是因為短程消化穩(wěn)定性未遭到破壞；鹽度為5.0%時,未檢測出是因為硝化反應被抑制。各鹽度條件下,SBBR懸浮污泥微生物活性均高于生物膜上微生物活性。(3)微生物胞外聚合物(EPS)分析結果表明,SBR活性污泥、SBBR中懸浮污泥及生物膜的可溶性微生物產(chǎn)物(SMP)、松散附著型EPS (LB-EPS)和緊密附著型EPS (TB-EPS)中的蛋白質(zhì)(PN)、多糖(PS)都隨鹽度的提高先增加后降低,均在鹽度為2.0%條件下達到最大值。鹽度在0.6%-5.0%范圍時,三種來源微生物的SMP、LB-EPS和TB-EPS樣品中均出現(xiàn)4個熒光峰,2個與類蛋白熒光有關,2個與類腐殖酸熒光有關。隨著鹽度的增加,三種來源微生物的LB-EPS樣品中類腐殖酸熒光有明顯變化,說明鹽度的增加對LB-EPS中腐殖酸的組成和結構有明顯的影響。SBBR中懸浮污泥EPS的類腐殖酸熒光峰變化比生物膜明顯(主要表現(xiàn)在LB-EPS).據(jù)EPS的傅里葉紅外光譜分析結果,鹽度在0.6%-5.0%范圍時,三種來源微生物的SMP、LB-EPS和TB-EPS紅外光譜吸收峰相似,但吸收峰的相對振動強度隨鹽度的增加而變化。
[Abstract]:The sequencing batch reactor activated sludge using the (SBR) and sequencing batch biofilm reactor (SBBR), studied the effect of salinity on pollutant removal and denitrification stability. (1) with complete nitrification sludge as seed sludge, gas supply in 0.6% salinity control (360 mL/min) and the reaction temperature (30 DEG C), starting 3 months two reactors have achieved efficient nitrification and denitrification. The influent salinity gradually increased from 1% to 5%, the increase of salinity of two reactor COD removal had obvious inhibition. The salinity in the range of 1.0%-4.5%, two the reactor effluent NH4+-N, NO2--N and N03--N were less than 1.0,0.5,0.5 mg/L, inhibited the increase of salinity is not produced during the nitrification process of two reactors and denitrification; the salinity range of SBR, nitrite accumulation rate (NAR) were more than 97% S. BBR NAR was above 95%. The water salinity increased to 5%, SBR and SBBR respectively, 9D and 7d after operation, the oxidation process of NH4+-N was almost completely inhibited, nitrification was completely destroyed. (2) the water salinity gradually increased to 5% from 0.6%, the sludge volume index SBR and SBBR (SVI) with the increase of salinity increases first and then decreases. The salinity in the range of 1.0%-3.0% SBR, activated sludge, suspended sludge and biofilm SBBR in microbial activity than the oxygen consumption rate (SOUR) decreased with the increase of salinity decreased gradually. The salinity in the range of 1.0%-5.0%, three kinds of microorganism than ammonia oxidation rate (SAOR), ratio of nitrite the reduction rate (SNIRR) and the ratio of nitrate reduction rate (SNARR) increased with the increase of salinity decreased to different extent. The salinity increase on the three sources of microbial inhibition of SAOR were significantly higher than the degree of inhibition of SNIRR and SNARR, NO2--N and N03--N that reducing bacteria Compared with the salt resistant ability of nitrifying bacteria is stronger. The salinity range, three sources of microbial SNIOR were not detected, the salinity range of 1.0%-3.0%, was not detected because of short digestion stability is not destroyed; the salinity of 5%, was not detected because denitrification should be suppressed. The salinity conditions suspended sludge, microbial activity of SBBR was higher than the activity of microbial biofilm. (3) microbial extracellular polymers (EPS) analysis showed that SBR activated sludge, soluble microbial products suspended sludge and biofilm in SBBR (SMP), loose adhesion type EPS (LB-EPS) and EPS (TB-EPS) closely attached. The protein (PN), polysaccharides (PS) with the increase of salinity increased first and then decreased, in 2% salinity conditions. Salinity reached the maximum value in the range of 0.6%-5.0%, three sources of microbial SMP, 4 fluorescence peaks appeared in the sample of LB-EPS and TB-EPS 2, and protein like fluorescence, 2 with humic fluorescence. With the increase of salinity, three sources of microbial samples of LB-EPS humic acid fluorescence changes obviously, indicating the increase of salinity of LB-EPS in the composition and structure of humic acid with humic like fluorescence peak changes of suspended sludge EPS.SBBR obviously than in biofilm (mainly in LB-EPS). According to the Fourier transform infrared spectroscopy EPS analysis results, the salinity in the range of 0.6%-5.0%, three sources of microbial SMP, LB-EPS and TB-EPS peak of infrared spectrum is similar, but the relative intensity of vibration absorption peak increased with salinity changes.

【學位授予單位】：中國海洋大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X703

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