本文選題：糖蛋白 + 硼酸��； 參考：《山東師范大學(xué)》2017年碩士論文

【摘要】：糖蛋白是生物體內(nèi)一種重要生物大分子,是蛋白質(zhì)糖基化修飾后的產(chǎn)物,在生物體內(nèi)發(fā)揮重要作用。研究表明,糖蛋白在生物體內(nèi)表達(dá)量的高低與多種疾病相關(guān):如轉(zhuǎn)鐵蛋白受體這種膜結(jié)合型糖蛋白,它的作用是與轉(zhuǎn)鐵蛋白結(jié)合,將鐵轉(zhuǎn)運(yùn)到細(xì)胞內(nèi),供細(xì)胞正常的生命活動(dòng),當(dāng)生物體發(fā)生癌變時(shí),轉(zhuǎn)鐵蛋白受體就會(huì)過量表達(dá),以此來(lái)轉(zhuǎn)運(yùn)腫瘤細(xì)胞惡性繁殖所需要的過量鐵;再如黏蛋白1這種膜結(jié)合型糖蛋白,正常情況下,它的作用是保持管道潤(rùn)滑、黏附管腔表面的細(xì)菌和病毒等,當(dāng)機(jī)體發(fā)生癌變時(shí),這種糖蛋白在細(xì)胞膜上的表達(dá)量也明顯增多。基于抗體識(shí)別技術(shù)雖具有良好的識(shí)別效果,但抗體存在制備步驟繁瑣、穩(wěn)定性差、價(jià)格昂貴等特點(diǎn)。因此,發(fā)展穩(wěn)定性好、操作簡(jiǎn)易、成本低廉、能快速分析糖蛋白的方法對(duì)研究相關(guān)疾病發(fā)生、發(fā)展有著重要意義。本論文主要設(shè)計(jì)制備了兩種熒光印跡納米材料,分別實(shí)現(xiàn)了對(duì)轉(zhuǎn)鐵蛋白和黏蛋白1的特異性識(shí)別。具體內(nèi)容如下:1、以包覆羅丹明B染料的二氧化硅為核,通過溶膠凝膠法在其表面修飾硼酸基團(tuán),以辣根過氧化物酶為模板制備了辣根過氧化物酶的印跡聚合物。為了驗(yàn)證方法的普適性,另選轉(zhuǎn)鐵蛋白為模板分子,制備了轉(zhuǎn)鐵蛋白的印跡聚合物。通過熒光響應(yīng)考察了印跡材料對(duì)模板分子轉(zhuǎn)鐵蛋白的識(shí)別性能,最后,以轉(zhuǎn)鐵蛋白為媒介,實(shí)現(xiàn)了細(xì)胞膜上轉(zhuǎn)鐵蛋白受體的特異性識(shí)別與熒光成像分析。該熒光印跡納米材料具有合成操作簡(jiǎn)易、識(shí)別速度快、穩(wěn)定性好等優(yōu)點(diǎn),更為重要的是,通過對(duì)轉(zhuǎn)鐵蛋白的特異性識(shí)別,實(shí)現(xiàn)了對(duì)轉(zhuǎn)鐵蛋白受體高表達(dá)相關(guān)循環(huán)腫瘤細(xì)胞的選擇性識(shí)別。該工作為科研工作者設(shè)計(jì)合成人工抗體提供了新思路。2、以上轉(zhuǎn)換納米材料為熒光團(tuán),在其表面修飾硼酸基團(tuán)。以黏蛋白1為模板,制備了黏蛋白1的印跡聚合物,通過熒光響應(yīng)考察了印跡材料的識(shí)別性能,并將該材料應(yīng)用于腫瘤細(xì)胞膜上黏蛋白1的特異性識(shí)別及成像分析研究。最后將其應(yīng)用于小鼠乳腺癌模型的治療,通過印跡材料的導(dǎo)向及在980 nm激光照射下的熱效應(yīng)實(shí)現(xiàn)對(duì)惡性增殖的腫瘤細(xì)胞的抑制及殺傷以達(dá)治療腫瘤的目的。實(shí)驗(yàn)過程及結(jié)果顯示,以上轉(zhuǎn)換為熒光團(tuán)的印跡聚合物具有背景干擾小、操作簡(jiǎn)便、能夠?qū)崿F(xiàn)腫瘤的識(shí)別與治療一體化等優(yōu)點(diǎn)。
[Abstract]:Glycoprotein is an important biological macromolecule in organism. It is the product of glycosylation of protein and plays an important role in organism. Studies have shown that the level of glycoprotein expression in organisms is related to many diseases: such as transferrin receptor, a membrane-binding glycoprotein, which binds to transferrin and transports iron into cells to supply normal cell life. When an organism becomes cancerous, the transferrin receptor is overexpressed to transport the excess iron needed for the malignant reproduction of the tumor cell, such as mucin 1, a membrane-binding glycoprotein, normally. Its function is to keep the pipeline lubricated, to adhere to the surface of the tube, bacteria and viruses, when the body carcinogenesis, the glycoprotein expression on the cell membrane is also significantly increased. Although the technology of antibody recognition has good recognition effect, the antibody has the characteristics of tedious preparation, poor stability and high price. Therefore, the method of rapid analysis of glycoprotein is of great significance in the study of the occurrence and development of related diseases. In this paper, two kinds of fluorescent imprinted nanomaterials were designed and prepared, and the specific recognition of transferrin and mucin 1 was realized respectively. The specific contents are as follows: 1. The imprinted polymer of horseradish peroxidase was prepared by sol-gel method with borate group modified on the surface of silica coated with Rhodamine B dye and horseradish peroxidase as template. In order to verify the universality of the method, the imprinted polymer of transferrin was prepared by using transferrin as template molecule. The recognition performance of template molecule transferrin by imprinted material was investigated by fluorescence response. Finally, the specific recognition and fluorescence imaging analysis of transferrin receptor on cell membrane were realized by using transferrin as the medium. The fluorescent imprinted nanomaterials have the advantages of simple synthesis, fast recognition, good stability, and, more importantly, the specific recognition of transferrin. Selective recognition of circulating tumor cells associated with high expression of transferrin receptor was achieved. This work provides a new way for researchers to design and synthesize artificial antibodies. The above conversion nanomaterials are fluorescent groups and modified borate groups on their surfaces. The imprinted polymer of mucin 1 was prepared by using mucin 1 as template. The recognition performance of the imprinted material was investigated by fluorescence response. The material was applied to the specific recognition and imaging analysis of mucin 1 on tumor cell membrane. Finally, it was applied to the treatment of mouse breast cancer model. The tumor cells were inhibited and killed by the guidance of imprinting material and the thermal effect of 980nm laser irradiation. The experimental process and results show that the imprinted polymer transformed into fluorescent group has the advantages of low background interference, simple operation, and the integration of tumor recognition and treatment.
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB383.1;O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 王錦;雷孝;何文禮;王超麗;;分子印跡技術(shù)的應(yīng)用進(jìn)展[J];化學(xué)與生物工程;2006年04期

2 陸書來(lái),成國(guó)祥,蔡志江,龐興收;蛋白質(zhì)模板印跡法制備納米“孔穴”結(jié)構(gòu)特異性生物材料[J];中國(guó)醫(yī)學(xué)科學(xué)院學(xué)報(bào);2003年05期

，

本文編號(hào)：2093750