本文選題：金納米粒子 + ATP　； 參考：《青島科技大學(xué)》2015年碩士論文

【摘要】：第一章,對(duì)相關(guān)技術(shù)背景進(jìn)行了詳細(xì)地介紹,比如金納米粒子的制備及其在生物醫(yī)學(xué)中的應(yīng)用、DNA、腫瘤細(xì)胞標(biāo)志物、電致化學(xué)發(fā)光、熒光及激光共聚焦顯微鏡等,了解了目前研究的現(xiàn)狀及檢測(cè)的技術(shù)水平,對(duì)自已研究的課題具有指導(dǎo)意義。最后介紹了本課題的研究目的及研究?jī)?nèi)容。第二章,制備金納米粒子(AuNRs),并且以金納米粒子作為標(biāo)記物,能夠和多種生物分子結(jié)合。通過對(duì)DNA的一些處理,建立了金納米粒子的功能化核酸適體的體系來檢測(cè)溶液中的ATP含量,并將此體系來與細(xì)胞結(jié)合并進(jìn)一步檢測(cè)細(xì)胞內(nèi)ATP的含量。檢測(cè)限為4.0×10.8 M濃度的ATP,并可以檢測(cè)200-5000個(gè)Ramos細(xì)胞。第三章,以合成的硫化鎘(CdS)量子點(diǎn)為基礎(chǔ),本章設(shè)計(jì)了一個(gè)基于CdS傳感器的電致化學(xué)發(fā)光法和熒光分光光度法來檢測(cè)ATP的含量的方法,對(duì)ATP有快速的響應(yīng)。當(dāng)體系中存在ATP時(shí),它就會(huì)和ATP適體鏈結(jié)合,從而釋放出CdS-DNA’鏈,CdS在包含0.1 M KC1和0.05 M K2S208的PBS緩沖溶液中能夠產(chǎn)生ECL信號(hào),通過ECL強(qiáng)度的改變,確定ATP的濃度。優(yōu)化實(shí)驗(yàn)條件后,此傳感器可以對(duì)2.0×10-9-1.0×10-7 M濃度范圍內(nèi)的ATP有響應(yīng)。也可以在金片上進(jìn)行修飾,就可以用于熒光的測(cè)量,對(duì)2.0×10.9-1.0×10-7M濃度范圍內(nèi)的ATP同樣有響應(yīng),因此此傳感器在生物分析領(lǐng)域有著很好的應(yīng)用前景。
[Abstract]:In the first chapter, the related technical background is introduced in detail, such as the preparation of gold nanoparticles and their applications in biomedical applications, such as DNA, tumor cell markers, electrochemiluminescence, fluorescence and laser confocal microscopy, etc. The present situation of research and the technical level of detection are understood, which is of guiding significance to the subject of our own research. Finally, the purpose and content of the research are introduced. In chapter 2, gold nanoparticles (AuNRs) were prepared, and gold nanoparticles were used as markers to bind to a variety of biomolecules. Through the treatment of DNA, a system of functional aptamer of gold nanoparticles was established to detect the ATP content in the solution, and the system was used to bind to the cells and further detect the ATP content in the cells. The detection limit was 4.0 脳 10.8 M, and 200-5000 Ramos cells could be detected. In chapter 3, based on the synthesized cadmium sulphide (CDs) quantum dots, we design a new method to detect ATP content by electrochemiluminescence and fluorescence spectrophotometry based on CDs sensor, which has a rapid response to ATP. When ATP is present in the system, it binds to the aptamer chain of ATP, thus releasing CdS-DNA 'chain CDs can produce ECL signal in PBS buffer solution containing 0.1 MKC1 and 0.05 MK2S208. The concentration of ATP is determined by changing the intensity of ECL. After optimizing the experimental conditions, the sensor can respond to ATP in the concentration range of 2.0 脳 10-9-1.0 脳 10-7 M. It can also be modified on a gold chip and can be used for fluorescence measurement. It is also responsive to ATP in the concentration range of 2.0 脳 10.9-1.0 脳 10 ~ (-7) M, so this sensor has a good application prospect in the field of biological analysis.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;TP212

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 邵桂妮;張興堂;劉兵;黃亞彬;杜祖亮;;溶膠凝膠-模板法制備一維金納米材料[J];現(xiàn)代化工;2006年01期

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本文編號(hào)：2094167