【摘要】：目的:研究天然低溫雨雪冰凍環(huán)境下暴露小鼠運動行為響應及其相關調(diào)控因子的變化,進而為該極端環(huán)境下如何進行作業(yè)保護的研究提供實驗依據(jù)。方法:挑選40只8周齡健康昆明種雄性小鼠,適應性喂養(yǎng)7天,篩選出符合要求的小鼠隨機分為4組:室溫環(huán)境下安靜對照組、運動對照組、低溫雨雪冰凍環(huán)境下安靜實驗組和運動實驗組。運動實驗組和安靜實驗組每天在低溫雨雪冰凍自然環(huán)境下暴露1小時。運動實驗組和運動對照組在暴露的1,3,5,7天做網(wǎng)屏和衣架實驗,用于反映小鼠的肌肉力量和協(xié)調(diào)能力變化;2,4,6天做吊尾實驗,反映小鼠的情緒變化。7天時安靜實驗組和安靜對照組即刻處死取材,運動實驗組和運動對照組在末次實驗后再做力竭游泳實驗(反映小鼠逃生欲望和剩余體能)后處死取材。留取各組小鼠的全腦和骨骼肌備用。檢測小鼠骨骼肌和腦的ACh E活性,腦IL-16含量。結果:(1)小鼠運動行為響應:(1)網(wǎng)屏實驗:運動實驗組滑落時間較運動對照組短,且在5天時滑落時間較長,7天時滑落時間最短。運動對照組在3、5、7天時滑落時間增加。(2)衣架實驗:與運動對照組相比,運動實驗組分數(shù)較小且在5天時分數(shù)增加,7天分數(shù)時又下降。(3)吊尾實驗:與運動對照組相比,運動實驗組掙扎時間較短,且隨著暴露天數(shù)增加掙扎時間下降。(4)力竭游泳實驗:與運動對照組相比,運動實驗組1分鐘游泳速度較大但游泳時間較短。(2)相關調(diào)控因子:(1)效應器官ACh E活性:與安靜對照組相比,運動對照組骨骼肌ACh E活性較低;與安靜實驗組相比,運動實驗組骨骼肌ACh E活性較低。(2)小鼠腦內(nèi)ACh E活性:與安靜對照組相比,運動對照組、安靜實驗組ACh E活性均較高;與運動對照組相比,運動實驗組腦ACh E活性較低;與安靜實驗組相比,運動實驗組腦內(nèi)ACh E活性較低。(3)腦內(nèi)IL-16的含量:與安靜對照組相比,運動對照組腦內(nèi)IL-16的含量較低;與運動對照組相比,運動實驗組腦內(nèi)IL-16的含量較低;與安靜實驗組相比,運動實驗組腦內(nèi)IL-16的含量較低。結論:1.低溫雨雪冰凍環(huán)境下小鼠運動行為響應:小鼠肌力減弱,協(xié)調(diào)性下降,掙扎時間較短,情緒反應降低,游泳實驗時第一分鐘內(nèi)逃生欲望增強,但總體運動耐力下降。2.調(diào)控因子變化:低溫雨雪冰凍環(huán)境對小鼠骨骼肌ACh E活性影響不明顯,力竭運動會使小鼠骨骼肌ACh E活性降低。低溫雨雪冰凍環(huán)境使小鼠腦內(nèi)興奮性神經(jīng)遞質(zhì)ACh含量降低;力竭運動使小鼠腦內(nèi)IL-16含量減少,小鼠免疫功能下降,且低溫雨雪冰凍環(huán)境下降低更明顯。
[Abstract]:Objective: To study the response of exercise behavior and related control factors of exposed mice in the environment of natural low temperature and rain and snow, and to provide the experimental basis for the research on how to carry out the operation protection in the extreme environment. Methods: Forty eight-week-old healthy Kunming male mice were selected, and the mice were randomly divided into 4 groups: a quiet control group, a motion control group, a quiet experimental group and a motion experimental group under the environment of room temperature. The experimental group and the quiet experimental group were exposed to a cold and snow-frozen natural environment for 1 hour each day. The experimental group and the exercise control group were used to screen and coat the exposed 1,3,5,7 days to reflect the changes of muscle strength and coordination ability of the mice. The experiment of the tail-tail was done in 2,4 and 6 days to reflect the changes of the mood of the mice. At 7 days, the experimental group and the quiet control group were killed immediately. The experimental group and the exercise control group were sacrificed after the last experiment and after the exhaustive swimming test (reflecting the desire and remaining physical ability of the mice). The whole brain and skeletal muscle were reserved for each group of mice. The activity of ACh E and the content of IL-16 in skeletal muscle and brain of mice were detected. Results: (1) Mouse exercise response: (1) Screen experiment: The sliding time of the moving experimental group was shorter than that of the control group, and the sliding time was longer than that of the control group at 5 days, and the sliding time at 7 days was the shortest. The sliding time of the control group was increased at 3,5 and 7 days. (2) The experiment of the coat hanger: Compared with the exercise control group, the score of the motion experimental group was small and the score increased at 5 days, and the score of 7 days decreased. (3) Suspension tail experiment: Compared with the exercise control group, the struggle time of the moving experimental group was short, and the struggle time decreased with the number of exposure days. (4) Exhaustive swimming test: Compared with the exercise control group, the swimming time of the exercise group is larger than that of the control group, but the swimming time is short. (2) Relevant regulatory factors: (1) the activity of ACh E in the effect organ: Compared with the control group, the activity of ACh E in the skeletal muscle of the exercise control group was lower; and the activity of ACh E in the skeletal muscle of the experimental group was lower than that of the experimental group. (2) The activity of ACh E in the brain of the mice was higher than that of the control group. The activity of ACh E in the experimental group was lower than that of the control group, and the activity of ACh E in the brain of the experimental group was lower than that of the experimental group. (3) The content of IL-16 in the brain of the control group was lower than that of the control group, the content of IL-16 in the brain of the experimental group was lower than that of the control group, and the content of IL-16 in the brain of the experimental group was lower than that of the experimental group. Conclusion:1. In that low-temperature and snow-frozen environment, the mouse exercise behavior response: the muscle strength of the mouse is weakened, the coordination is reduced, the struggle time is short, the emotional response is reduced, and the escape desire in the first minute is enhanced in the swimming experiment, but the overall movement endurance is reduced. The changes of the control factors: The effect of low-temperature and snow-freezing environment on the activity of ACh E in the skeletal muscle of the mouse was not obvious, and the activity of ACh E in the skeletal muscle of the mouse was reduced by the exhaustive exercise. In that low-temperature and snow-freezing environment, the content of the excitatory neurotransmitter ACh in the brain of the mouse is reduced, the content of IL-16 in the brain of the mouse is reduced by the exhaustive exercise, the immune function of the mouse is reduced, and the decrease of the low-temperature snow and snow freezing environment is more obvious.
【學位授予單位】：沈陽師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：G80-05

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