【摘要】：1目的觀察低氧運(yùn)動(dòng)對(duì)糖尿病大鼠骨骼肌中胰島素信號(hào)傳導(dǎo)途徑中相關(guān)蛋白含量變化,探究低氧運(yùn)動(dòng)對(duì)糖尿病治療作用可能機(jī)制。2方法將60只6~8周齡SD大鼠隨機(jī)挑選10只作為正常對(duì)照組。其余50只大鼠4周高脂膳食飼養(yǎng),一次性腹腔注射鏈脲佐菌素(STZ)誘導(dǎo)糖尿病模型,空腹血糖≥16.7mmol/L為成模標(biāo)準(zhǔn)。將建模成功的糖尿病30只大鼠隨機(jī)分為3組,分別為低氧運(yùn)動(dòng)組、常氧運(yùn)動(dòng)組和糖尿病組,每組各10只。低氧環(huán)境采用低氧帳篷(MAG10)模擬海拔2500m高度,氧濃度15.0%。運(yùn)動(dòng)組3d跑臺(tái)適應(yīng)性訓(xùn)練之后正式開(kāi)始訓(xùn)練,訓(xùn)練4周,每周6天,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組每天進(jìn)行1h跑臺(tái)訓(xùn)練。最后一次運(yùn)動(dòng)后大鼠禁食12小時(shí),經(jīng)腹腔注射戊巴比妥鈉麻醉之后腹腔主動(dòng)脈取血,解剖大鼠股四頭肌。測(cè)血液中胰島素(Ins),股四頭肌中葡萄糖轉(zhuǎn)運(yùn)蛋白(GLUT4)、磷酸活化腺苷激酶(AMPK)、磷脂酰肌醇-3激酶(PI3K)和蛋白激酶(PKB)含量,所有數(shù)據(jù)用SPSS19.0軟件進(jìn)行單因素方差分析,實(shí)驗(yàn)結(jié)果用平均數(shù)±標(biāo)準(zhǔn)差表示。3結(jié)果(1)與正常對(duì)照組相比,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組GLUT4含量顯著降低(p0.05)。與糖尿病組相比,常氧運(yùn)動(dòng)組和低氧運(yùn)動(dòng)組GLUT4含量顯著增加(p0.05)。與低氧運(yùn)動(dòng)組相比,常氧運(yùn)動(dòng)組GLUT4含量略低,但沒(méi)有顯著性差異(P0.05)。(2)與正常對(duì)照組相比,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組PI3K活性非常顯著性降低(P0.01)。與糖尿病組相比,常氧運(yùn)動(dòng)組和低氧運(yùn)動(dòng)組PI3K活性非常顯著性增加(P0.01)。與低氧運(yùn)動(dòng)組相比,常氧運(yùn)動(dòng)組PI3K活性顯著降低(P0.05)。(3)與正常對(duì)照組相比,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組PKB活性非常顯著性降低(P0.01)。與糖尿病組相比,常氧運(yùn)動(dòng)組和低氧運(yùn)動(dòng)組PKB活性非常顯著性增加(P0.01)。與低氧運(yùn)動(dòng)組相比,常氧運(yùn)動(dòng)組PKB活性顯著降低(P0.05)。(4)與正常對(duì)照組相比,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組AMPK活性顯著性降低(P0.05)。與糖尿病組相比,常氧運(yùn)動(dòng)組和低氧運(yùn)動(dòng)組AMPK活性非常顯著性增加(P0.01)。與低氧運(yùn)動(dòng)組相比,常氧運(yùn)動(dòng)組AMPK活性顯著降低(P0.05)。(5)與正常對(duì)照組相比,低氧運(yùn)動(dòng)組和常氧運(yùn)動(dòng)組胰島素含量極其顯著性降低(P0.01)。與糖尿病組相比,常氧運(yùn)動(dòng)組和低氧運(yùn)動(dòng)組胰島素含量顯著性增加(P0.05)。與低氧運(yùn)動(dòng)組相比,常氧運(yùn)動(dòng)組胰島素含量非常顯著性降低(P0.01)。4結(jié)論低氧運(yùn)動(dòng)和常氧運(yùn)動(dòng)都能能顯著提高糖尿病大鼠骨骼肌PI3K、PKB、AMPK活性及GLUT4和胰島素含量,但是低氧運(yùn)動(dòng)刺激對(duì)糖尿病大鼠葡萄糖轉(zhuǎn)運(yùn)影響更顯著。
[Abstract]:Objective to observe the changes of related proteins in insulin signal transduction pathway in skeletal muscle of diabetic rats after hypoxic exercise. To explore the possible mechanism of hypoxic exercise in the treatment of diabetes mellitus. (2) 60 SD rats aged 68 weeks were randomly selected as the normal control group. The other 50 rats were fed with high-fat diet for 4 weeks. Streptozotocin (STZ) was injected intraperitoneally to induce diabetic model. Fasting blood glucose 鈮，

本文編號(hào)：2284281