運動訓練通過Nrf2對骨骼肌抗氧化蛋白作用的影響
發(fā)布時間：2018-08-21 12:28
【摘要】：研究目的:運動訓練能夠提高機體的抗氧化能力。Nrf2作為機體氧化還原平衡的主要調(diào)節(jié)因子。運動訓練是否會影響Nrf2及抗氧化蛋白表達,進而影響體內(nèi)氧化還原狀態(tài),目前還不清楚。本實驗通過對野生小鼠和Nrf2敲除小鼠在安靜和運動兩種情況下,骨骼肌細胞中Nrf2及抗氧化蛋白表達的檢測,探討運動訓練對骨骼肌中Nrf2與抗氧化蛋白表達的影響。研究方法:野生C57BL/6J小鼠和Nrf2敲除小鼠各20只,根據(jù)體重,兩種鼠分為2組,每組10只,雌雄各半,分別為安靜組和運動組,共4組。運動組進行四周跑臺運動,每周運動6天,休息一天,每天運動1h,速度12m/min。正式實驗前三天,運動組小鼠進行20min適應(yīng)性的跑臺運動。在整個運動過程中未對動物使用任何電刺激。運動組進行最后一次運動之后休息48小時后同安靜組同脫頸處死,取材。Western blot法測定骨骼肌胞漿蛋白中4-HNE、Nrf2及抗氧化蛋白含量;RT-PCR測量Nrf2調(diào)節(jié)的抗氧化基因的表達量;ROS試劑盒測量骨骼肌內(nèi)ROS含量。研究結(jié)果:1)在安靜情況下,野生鼠和Nrf2敲除小鼠相比,骨骼肌中ROS以及CAT、NQO1、GCLc、HO-1和SOD2這五個抗氧化蛋白沒有顯著性改變。2)四周運動訓練之后,Nrf2敲除運動組的ROS和大部分抗氧化蛋白都要顯著低于野生運動組。研究結(jié)論:運動訓練通過Nrf2促進小鼠骨骼肌抗氧化基因以及蛋白的表達。
[Abstract]:Objective: exercise training can improve the antioxidant ability of the body. Nrf2 is the main regulatory factor of redox balance. It is not clear whether exercise training will affect the expression of Nrf2 and antioxidant protein, and then the redox state in vivo. The effects of exercise training on the expression of Nrf2 and antioxidant protein in skeletal muscle cells were investigated by detecting the expression of Nrf2 and antioxidant protein in skeletal muscle cells of wild mice and Nrf2 knockout mice. Methods: there were 20 wild C57BL/6J mice and 20 Nrf2 knockout mice. According to their body weight, two kinds of mice were divided into 2 groups, 10 rats in each group, half male and half female, divided into four groups: quiet group and exercise group. The exercise group ran around the platform, 6 days a week, a day off, 1 hour a day, at a speed of 12 m / min. Three days before the formal experiment, the mice in the exercise group underwent 20min adaptive treadmill exercise. No electrical stimulation was used during the whole exercise. The exercise group took a rest for 48 hours after the last exercise and was executed with the same neck removal as the rest group. Determination of 4-HNE-Nrf2 in cytosolic protein of skeletal muscle by Western blot method and determination of antioxidant protein content by RT-PCR the expression of antioxidant gene regulated by Nrf2 was measured by Ros kit. ROS content in skeletal muscle was measured by RT-PCR. Results: 1) in quiet conditions, wild mice were compared with Nrf2 knockout mice. There was no significant change in ROS and SOD2 in skeletal muscle. The ROS and most antioxidant proteins in NRF 2 knockout exercise group were significantly lower than those in wild exercise group. Conclusion: exercise training can promote the expression of antioxidant genes and proteins in skeletal muscle of mice by Nrf2.
【學位授予單位】：北京體育大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：G804.7
本文編號：2195742
[Abstract]:Objective: exercise training can improve the antioxidant ability of the body. Nrf2 is the main regulatory factor of redox balance. It is not clear whether exercise training will affect the expression of Nrf2 and antioxidant protein, and then the redox state in vivo. The effects of exercise training on the expression of Nrf2 and antioxidant protein in skeletal muscle cells were investigated by detecting the expression of Nrf2 and antioxidant protein in skeletal muscle cells of wild mice and Nrf2 knockout mice. Methods: there were 20 wild C57BL/6J mice and 20 Nrf2 knockout mice. According to their body weight, two kinds of mice were divided into 2 groups, 10 rats in each group, half male and half female, divided into four groups: quiet group and exercise group. The exercise group ran around the platform, 6 days a week, a day off, 1 hour a day, at a speed of 12 m / min. Three days before the formal experiment, the mice in the exercise group underwent 20min adaptive treadmill exercise. No electrical stimulation was used during the whole exercise. The exercise group took a rest for 48 hours after the last exercise and was executed with the same neck removal as the rest group. Determination of 4-HNE-Nrf2 in cytosolic protein of skeletal muscle by Western blot method and determination of antioxidant protein content by RT-PCR the expression of antioxidant gene regulated by Nrf2 was measured by Ros kit. ROS content in skeletal muscle was measured by RT-PCR. Results: 1) in quiet conditions, wild mice were compared with Nrf2 knockout mice. There was no significant change in ROS and SOD2 in skeletal muscle. The ROS and most antioxidant proteins in NRF 2 knockout exercise group were significantly lower than those in wild exercise group. Conclusion: exercise training can promote the expression of antioxidant genes and proteins in skeletal muscle of mice by Nrf2.
【學位授予單位】：北京體育大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：G804.7
【相似文獻】
相關(guān)會議論文 前2條
1 茍巧;佟鵬;王春燕;張翠蘭;蘇旭;;α粒子誘發(fā)人支氣管上皮細胞BEP2D惡性轉(zhuǎn)化中細胞內(nèi)抗氧化蛋白和活性氧水平研究[A];低碳生活與健康損害論壇——中國環(huán)境誘變劑學會風險評價專業(yè)委員會全國第十三屆學術(shù)會議暨第四屆第5次委員會會議論文集[C];2011年
2 佟鵬;茍巧;;α粒子誘發(fā)人支氣管上皮細胞BEP2D惡性轉(zhuǎn)化中細胞內(nèi)抗氧化蛋白和活性氧水平研究[A];中國毒理學會第三屆中青年學者科技論壇暨2011年全國前列腺藥理毒理學研討會論文集[C];2011年
相關(guān)碩士學位論文 前5條
1 侯楠;擬南芥抗氧化蛋白的體外純化及抗氧化特性初步研究[D];南陽師范學院;2015年
2 何燕;抗氧化蛋白Prdx6在食管癌中的表達及生物學功能研究[D];蘇州大學;2016年
3 唐正江;雞胚抗氧化蛋白的分離純化及性質(zhì)研究[D];合肥工業(yè)大學;2017年
4 何詩依;運動訓練通過Nrf2對骨骼肌抗氧化蛋白作用的影響[D];北京體育大學;2017年
5 顏晟;人抗氧化蛋白Peroxiredoxin 1和2的表達、純化及功能研究[D];華南理工大學;2012年
，本文編號：2195742
本文鏈接：http://sikaile.net/jiaoyulunwen/tylw/2195742.html
最近更新
教材專著
