【摘要】：1.目的通過觀察常氧和低氧運(yùn)動(dòng)對(duì)Ⅱ型糖尿病大鼠糖脂代謝相關(guān)指標(biāo)的影響,探討常氧和低氧運(yùn)動(dòng)對(duì)Ⅱ型糖尿病的作用及可能機(jī)制。2.方法選取60只6~8周齡雄性SD大鼠作為實(shí)驗(yàn)對(duì)象,隨機(jī)取10只作正常對(duì)照組,其余經(jīng)高糖高脂飼料喂養(yǎng)4周后,腹腔注射鏈脈佐菌素(STZ)建立Ⅱ型糖尿病大鼠模型(空腹血糖"g16.7mmol·L-1為成模標(biāo)準(zhǔn))。將建模成功的Ⅱ型糖尿病大鼠再隨機(jī)分為糖尿病組、糖尿病常氧運(yùn)動(dòng)組和糖尿病低氧運(yùn)動(dòng)組,每組各10只。后兩組分別進(jìn)行為期4周跑臺(tái)運(yùn)動(dòng)訓(xùn)練(坡度0°,速度15m·min-1,60 min·d-1,6d·W-1),糖尿病低氧運(yùn)動(dòng)組在低氧帳篷(模擬海拔2500m,氧濃度15.0%的氧環(huán)境)中運(yùn)動(dòng)。末次運(yùn)動(dòng)后,所有大鼠禁食不禁水12h,經(jīng)麻醉后,腹腔取血、肝臟、腎周脂肪。測(cè)定大鼠空腹血糖(FBG)、空腹胰島素(FINS)、血清總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL-C)、肝臟組織中胰島素受體蛋白(InsR)、葡萄糖激酶(GCK)、內(nèi)臟脂肪組織中葡萄糖轉(zhuǎn)運(yùn)蛋白(GLUT-4)的含量。3.結(jié)果(1)經(jīng)過4周運(yùn)動(dòng)干預(yù),糖尿病常氧運(yùn)動(dòng)組和糖尿病低氧運(yùn)動(dòng)組大鼠癥狀較糖尿病組均有所減輕,其中,糖尿病低氧運(yùn)動(dòng)組效果較為明顯。(2)與正常對(duì)照組相比,糖尿病組大鼠血清TC、TG和LDL-C均非常顯著升高(P0.01);與糖尿病組相比,糖尿病常氧運(yùn)動(dòng)組大鼠血清LDL-C及TC均非常顯著降低(P0.01),糖尿病低氧運(yùn)動(dòng)組大鼠血清LDL-C、TC和TG均非常顯著降低(P0.01),HDL明顯升高(P0.05);與糖尿病低氧運(yùn)動(dòng)組相比,糖尿病常氧運(yùn)動(dòng)組大鼠血清TC及LDL-C均較高(P0.05),HDL較低(P0.05),TG明顯較高(P0.05)。(3)與正常對(duì)照組相比,糖尿病組FBG顯著升高(P0.05);與糖尿病組相比,糖尿病常氧運(yùn)動(dòng)組和糖尿病低氧運(yùn)動(dòng)組FBG均非常顯著降低(P0.01);與糖尿病低氧運(yùn)動(dòng)組相比,糖尿病常氧運(yùn)動(dòng)組FBG較高(P0.05)。(4)與正常對(duì)照組相比,糖尿病組FINS顯著升高(P0.05);與糖尿病組相比,糖尿病常氧運(yùn)動(dòng)組和糖尿病低氧運(yùn)動(dòng)組FINS均非常顯著降低(P0.01);與糖尿病低氧運(yùn)動(dòng)組相比,糖尿病常氧運(yùn)動(dòng)組FINS明顯升高(P0.05)。(5)與正常對(duì)照組相比,糖尿病組肝臟中InsR、GCK以及內(nèi)臟脂肪組織中GLUT-4含量均非常顯著降低(P0.01);與糖尿病組相比,糖尿病常氧運(yùn)動(dòng)組和糖尿病低氧運(yùn)動(dòng)組肝臟組織中InsR、GCK以及GLUT-4含量均明顯升高(P0.05);與糖尿病低氧運(yùn)動(dòng)組相比,糖尿病常氧運(yùn)動(dòng)組GCK含量顯著降低(P0.05)。4.結(jié)論常氧和低氧運(yùn)動(dòng)均可降低Ⅱ型糖尿病大鼠空腹血糖及空腹胰島素濃度,減少血清TG、TC和LDL-C含量,升高HDL含量,增加肝組織中InsR,GCK以及內(nèi)臟脂肪組織中GLUT-4的含量,有利于促進(jìn)Ⅱ型糖尿病大鼠糖脂代謝紊亂的恢復(fù),且低氧運(yùn)動(dòng)干預(yù)效果優(yōu)于常氧運(yùn)動(dòng)。
[Abstract]:1. Objective to observe the effects of normoxic and hypoxic exercise on glucose and lipid metabolism in type 2 diabetic rats, and to explore the effect of normoxic and hypoxic exercise on type 2 diabetes mellitus and its possible mechanism. Methods 60 male Sprague-Dawley (SD) rats aged 6 weeks and 8 weeks old were selected as experimental objects, 10 rats were randomly selected as normal control group, and the rest were fed with high sugar and high fat diet for 4 weeks. The rat model of type 2 diabetes was established by intraperitoneal injection of streptozotocin (STZ). Fasting blood glucose (g16.7mmol L-1) was used as the model standard. The successful type 2 diabetic rats were randomly divided into diabetic group, diabetic normoxic exercise group and diabetic hypoxic exercise group with 10 rats in each group. The latter two groups were trained in treadmill training for 4 weeks (slope 0 擄, speed 15m min-1 60 min d-1d W-1), and diabetic hypoxic exercise group exercised in hypoxic tents (simulated altitude 2500m, oxygen concentration 15.0%). After the last exercise, all rats fasted for 12 hours. After anaesthesia, blood, liver and perirenal fat were taken from abdominal cavity. Fasting blood glucose (FBG), fasting insulin (fins), serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL-C), insulin receptor protein (InsR), glucose kinase (GCK) and glucose in visceral adipose tissue were measured in rats. The content of transporter protein (GLUT-4). Results (1) after 4 weeks of exercise intervention, the symptoms of diabetic normoxic exercise group and diabetic hypoxic exercise group were all alleviated compared with diabetic group, and the effect of diabetic hypoxic exercise group was more obvious than that of normal control group. The levels of TG and LDL-C in serum of diabetic rats were significantly higher than those in diabetic group (P0.01). Serum LDL-C and TC were significantly decreased in diabetic normoxic exercise group (P0.01), and serum LDL-CU TC and TG were significantly decreased (P0.01) in diabetic hypoxic exercise group (P0.01), compared with diabetic hypoxic exercise group, LDL-C and TC were significantly increased (P0.05). The serum TC and LDL-C of diabetic rats in normoxic exercise group were higher than those in normal exercise group (P0.05), the TG of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of normal control group (P0.05), and that of diabetic group was significantly higher than that of diabetic group (P0.05). Compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group had a higher FBG (P0.05). (4) than the normal control group (P0.05), the diabetic exercise group significantly increased fins (P0.05), and compared with the diabetic hypoxic exercise group (P0.01), the diabetic normoxic exercise group was significantly higher than the diabetic hypoxic exercise group (P0.01), and compared with the diabetic hypoxic exercise group, the diabetic normoxic exercise group was significantly higher than the control group (P0.05). Fins in diabetic normoxic exercise group and diabetic hypoxic exercise group was significantly decreased (P0.01), and fins in diabetic normoxic exercise group was significantly higher than that in diabetic hypoxic exercise group (P0.05). (5) compared with normal control group. The contents of InsRGCK in liver and GLUT-4 in visceral adipose tissue in diabetic group were significantly lower than those in diabetic group (P0.01). The contents of InsRGCK and GLUT-4 in liver tissue of diabetic normoxic exercise group and diabetic hypoxic exercise group were significantly higher than those of diabetic hypoxic exercise group (P0.05), and the GCK content of diabetic normoxic exercise group was significantly lower than that of diabetic hypoxic exercise group (P0.05). 4. Conclusion both normoxic and hypoxic exercise can decrease fasting blood glucose and fasting insulin, decrease serum TGTC and LDL-C, increase HDL content, increase the content of InsRGCK in liver and GLUT-4 in visceral adipose tissue. It is beneficial to promote the recovery of glucose and lipid metabolism disorder in type 2 diabetic rats, and the effect of hypoxic exercise intervention is better than that of normoxic exercise.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：G804.7

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