本文選題：肌肉工作形式 + 運動��； 參考：《河北師范大學》2017年碩士論文

【摘要】：研究目的:運動誘導骨骼肌分泌脂代謝調(diào)節(jié)肌肉因子,進而促進脂肪氧化燃燒是近年來運動減肥機制的研究熱點,本實驗探討相同攝氧量下,兩種肌肉工作形式(向心運動和離心運動)運動后對血清Irisin、CTRP15的不同影響,以揭示相同能量代謝水平下,不同運動形式對脂肪氧化調(diào)控的區(qū)別。研究方法:研究選用純系雄性Sprague-Dawley(SD)大鼠90只,8周齡,體重200±20g。大鼠隨機分為對照組(n=6)、平坡跑運動組(n=30)和下坡跑運動組(n=30)。運動組的運動時間都為60分鐘,下坡跑運動組的坡度為-5°。在運動結(jié)束后的0h、6h、12h、24h和48h每組取6只大鼠分別進行心臟取血、腓腸肌和比目魚肌取材,檢測各時間點FNDC5水平。采用ELISA方法檢測大鼠血清Irisin水平和CTRP15的水平。研究結(jié)果:(1)運動時間相同,跑速有別情況下,大鼠平坡跑和下坡跑的攝氧量水平可以無統(tǒng)計學差異(P0.05)。(2)向心運動組和離心運動組大鼠運動后6h,腓腸肌FNDC5的表達水平顯著增加(P0.05);向心運動組運動后0h、12h、24h、48h與對照組相比,沒有顯著性差異;離心運動組運動后0h、12h、24h、48h與對照組相比,顯著提高。(3)大鼠運動后血清Irisin水平的比較:向心運動組與對照組相比,運動后0h(15.73±1.31 ng/mL)、6h(18.66±1.57ng/mL)以及24h(16.19±1.08ng/mL)血清Irisin水平明顯高于對照組血清Irisin水平(13.80±0.84ng/mL),(P0.05)。離心運動與對照組相比,運動后0h(16.37±1.34 ng/mL)、12h(25.37±1.63 ng/mL)、24h(15.60±1.63ng/mL)以及48h(34.45±3.30 ng/mL)血清Irisin水平明顯高于對照組血清Irisin水平(13.80±0.84 ng/mL),(P0.01)。(4)運動后血清CTRP15血清水平的比較:向心運動組與對照組相比,運動后0h、6h、12h、24h、48h,血清CTRP15水平明顯升高(P0.05)。離心運動除運動后6h,沒有明顯升高外,其它各時間點均有顯著提高(P0.01)。結(jié)論:兩種不同形式的運動(向心運動和離心運動)都能促進骨骼肌FNDC5的表達水平和血清Irisin、CTRP15的表達,向心運動后FNDC5與Irisin只出現(xiàn)了一次峰值,離心運動后FNDC5和Irisin出現(xiàn)兩次峰值,且持續(xù)時間更長,釋放的肌肉因子更多,效果更明顯,更有利于運動減肥。
[Abstract]:Objective: exercise induced lipid metabolism in skeletal muscles to regulate muscle factors and thus promote fat oxidation is a hot topic in recent years. The aim of this study was to study the mechanism of exercise weight loss under the same oxygen intake. The effects of two muscle working forms (concentric exercise and centrifugal exercise) on serum Irisinine CTRP15 were studied in order to reveal the difference of lipid oxidation under the same energy metabolism level. Methods: 90 male Sprague-Dawley SD rats of 8 weeks old and weight of 200 鹵20 g were used in this study. Rats were randomly divided into two groups: control group (n = 6), flat slope running group (n = 30) and downhill running group (n = 30). The exercise time of the exercise group was 60 minutes, and the slope of the downhill running group was-5 擄. Six rats in each group were collected blood samples from heart, gastrocnemius muscle and soleus muscle at 0 h, 6 h and 12 h after exercise for 24 h and 48 h, respectively. The FNDC5 level was measured at each time point. Serum Irisin and CTRP15 levels were measured by Elisa. The results of the study were as follows: (1) when the exercise time is the same and the running speed is different, There was no statistical difference in oxygen uptake between downhill and flat slope running rats (P 0.05) the expression of FNDC5 in gastrocnemius muscle increased significantly 6 hours after exercise in the concentric exercise group and the centrifugal exercise group, and the expression of FNDC5 in the gastrocnemius muscle in the concentric exercise group was significantly higher than that in the control group at 12 hours and 24 hours after the exercise, compared with that in the control group, and the expression of FNDC5 in the gastrocnemius muscle of the rats in the concentric exercise group was significantly higher than that in the control group. Compared with the control group, the serum Irisin level in the centrifuge exercise group was significantly higher than that in the control group, and the serum Irisin level in the concentric exercise group was significantly higher than that in the control group, and that in the concentric exercise group was higher than that in the control group. After exercise, the serum Irisin levels of 0h(15.73 鹵1.31 ng / mL + 6 h, 18.66 鹵1.57 ng / mL and 24h(16.19 鹵1.08ng / mL) were significantly higher than those of the control group (13.80 鹵0.84ng / mL). Compared with the control group, the serum Irisin levels of 0h(16.37 鹵1.34ng / mL after exercise were significantly higher than those of the control group (13.80 鹵0.84 ng / mL) after exercise: compared with that of the control group: the serum levels of CTRP15 in the concentric exercise group were significantly higher than those in the control group (25.37 鹵1.63 ng / mL) and in the concentric exercise group and the control group, respectively, compared with those in the control group, and compared with those in the control group, the results showed that the serum Irisin level in the concentric exercise group was significantly higher than that in the control group (P < 0.05), and was significantly higher than that in the control group (P < 0.05). The serum levels of CTRP15 increased significantly at 0 h, 6 h, 12 h, 24 h and 48 h after exercise. Except for 6 hours after eccentric exercise, there was no significant increase in P0. 01, except that at other time points, there was a significant increase in P0. 01% at other time points. Conclusion: two different forms of exercise (concentric exercise and eccentric exercise) can promote the expression of FNDC5 in skeletal muscle and the expression of serum Irisinon CTRP15. There is only one peak value of FNDC5 and Irisin after concentric exercise, and two peaks of FNDC5 and Irisin after centrifugal exercise. And longer duration, the release of more muscle factors, more obvious effect, more conducive to exercise weight loss.
【學位授予單位】：河北師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：G804.7

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本文編號：2022239