發(fā)布時(shí)間：2018-04-30 17:32

  本文選題：炎癥因子 + chemerin��； 參考：《上海體育學(xué)院學(xué)報(bào)》2017年04期

【摘要】：目的:研究炎癥因子chemerin在有氧運(yùn)動(dòng)改善動(dòng)脈粥樣硬化大鼠血脂和主動(dòng)脈硬化中的作用。方法:將30只雄性SD大鼠隨機(jī)分為2組,AS造模組20只,采用一次性腹腔注射維生素D3(60萬(wàn)IU/kg)聯(lián)合8周高脂飼料喂養(yǎng)的方法制備AS大鼠模型;正常對(duì)照組(Con)10只,腹腔一次性注射等量的生理鹽水,并喂以8周普通飼料。建模成功的AS大鼠被隨機(jī)分為AS組和運(yùn)動(dòng)+AS(EAS)組。EAS組大鼠在跑臺(tái)上進(jìn)行為期4周中等強(qiáng)度的有氧運(yùn)動(dòng),每周運(yùn)動(dòng)6 d。油紅O染色和HE染色檢測(cè)AS程度,全自動(dòng)生化分析儀檢測(cè)大鼠血脂(血清TC、TG、LDL、HDL水平),ELISA檢測(cè)血清chemerin水平,以及real time PCR和Western blot檢測(cè)大鼠肝臟chemerin的mRNA和蛋白表達(dá)水平。結(jié)果:(1)與Con組相比,AS組大鼠出現(xiàn)血脂紊亂(血清TC、TG、LDL水平顯著升高,HDL顯著降低),以及主動(dòng)脈粥樣斑塊,斑塊處血管中膜的平滑肌細(xì)胞增生、排列紊亂,但AS組大鼠的空腹血糖無(wú)變化。(2)與AS組相比,EAS組大鼠的TC、TG、LDL水平顯著降低、主動(dòng)脈AS的大體和鏡下病理改變均顯著減輕;腎周脂肪量顯著減少,以及空腹血糖無(wú)變化。(3)與Con組相比,AS組大鼠的血清chemerin、肝chemerin的mRNA和蛋白水平均顯著升高。(4)與AS組相比,EAS組大鼠的血清chemerin、肝chemerin的mRNA和蛋白水平均顯著降低。結(jié)論:AS大鼠的血清chemerin、肝chemerin的mRNA和蛋白水平均顯著升高;4周有氧運(yùn)動(dòng)改善AS大鼠的血脂、減少腎周脂肪量和減輕AS程度,這可能與其降低血清和肝的chemerin水平有關(guān)。
[Abstract]:Aim: to study the role of inflammatory factor chemerin in improving blood lipid and aortic atherosclerosis in rats with atherosclerosis by aerobic exercise. Methods: thirty male Sprague-Dawley rats were randomly divided into two groups: control group (n = 20) and control group (n = 10). Intraperitoneal injection of the same amount of normal saline, and fed with 8 weeks of normal diet. The successful as rats were randomly divided into as group and ASE ASA group. EAS group performed moderate aerobic exercise for 4 weeks on the treadmill for 6 days per week. The degree of as was detected by oil red O staining and HE staining. Serum lipids were detected by automatic biochemical analyzer. Serum chemerin level was detected by Elisa, and mRNA and protein expression of rat liver chemerin were detected by real time PCR and Western blot. Results compared with the Con group, the rats in the as group showed dyslipidemia (the level of TCT-TGP LDL was significantly increased, and the atherosclerotic plaques in the aorta, smooth muscle cells of the vascular mesenchymal membrane in the plaques were proliferated and disordered. However, the fasting blood glucose in as group was not changed. (2) compared with as group, the level of TCN TGN LDL in EAS group was significantly decreased, the gross and pathological changes in aorta as were significantly reduced, and the perirenal fat content was significantly decreased. The levels of serum chemerin, liver chemerin mRNA and protein in as group were significantly higher than those in Con group. The levels of serum chemerin, mRNA and protein in chemerin group were significantly lower than those in as group. Conclusion the levels of serum chemerin, mRNA and protein of chemerin in liver of as rats were significantly higher than those of control rats. Aerobic exercise for 4 weeks could improve blood lipid, decrease the amount of perirenal fat and reduce the degree of as in as rats, which might be related to the decrease of chemerin level in serum and liver.
【作者單位】： 上海體育學(xué)院運(yùn)動(dòng)科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(31271274)
【分類號(hào)】：G804.2

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