本文選題：β-丙氨酸　切入點：肌肽　出處：《北京體育大學(xué)》2015年碩士論文

【摘要】：目的：通過給間歇運動訓(xùn)練的大鼠補充p-丙氨酸,觀察大鼠骨骼肌中肌肽含量的變化,探索補充p-丙氨酸對間歇訓(xùn)練大鼠骨骼肌以及肝臟糖代謝的影響。方法：將40只八周齡的健康雄性SD大鼠,隨機分成4組(N=10)：間歇運動中等劑量補劑組(A組)、間歇運動高劑量補劑組(B組)、間歇運動補水組(C組)、空白對照組(D組)。運動方式采用高速間歇訓(xùn)練,跑臺速度為50 m/min,時間6 min,休息5 min后,繼續(xù)依此方式共訓(xùn)練3次,每周6天,共4周。補劑采用灌胃方式,中等劑量組補充劑量為1g/kg體重,高劑量組補充劑量為1.5g/kg體重,補水組和空白對照組則為相同體積的蒸餾水。然后采用鄰苯二甲醛柱前衍生化法測試腓腸肌中肌肽含量、比色法測腓腸肌肌糖原含量、比色法測腓腸肌己糖激酶活性；葡萄糖氧化酶法測血清中葡萄糖含量；比色法測肝臟中肝糖原含量以及實時熒光定量PCR測肝臟葡萄糖-6-磷酸酶、糖皮質(zhì)激素受體、糖原合成酶、葡萄糖激酶和胰島素受體的mRNA相對表達。結(jié)果：(1)A組大鼠腓腸肌中肌肽含量顯著高于C組(p0.05),B組也顯著高于C組(p0.01)；C組大鼠腓腸肌己糖激酶活性顯著低于D組,A組顯著高于B、C兩組(p0.01)。(2)C組的大鼠肝糖原含量顯著高于D組(p0.01),A組顯著低于B、C兩組(p0.05)；C組大鼠肝臟中葡糖糖-6-磷酸酶的mRNA相對表達顯著高于D組(p0.01)；A組大鼠肝臟中糖皮質(zhì)激素受體的mRNA相對表達顯著高于B、C兩組(p0.01)；C組大鼠肝臟中糖原合成酶的mRNA相對表達顯著低于D組(p0.01)；A組大鼠胰島素受體的mRNA相對表達顯著高于B組(p0.01)。結(jié)論：補充p-丙氨酸可以促進間歇訓(xùn)練大鼠肌肉對血糖的利用,增加肝臟糖原的分解,中等劑量效果更好。
[Abstract]:Objective: to observe the change of carnosine content in skeletal muscle of rats by adding palanine to intermittent exercise training rats. To explore the effect of supplementation of p-alanine on glucose metabolism in skeletal muscle and liver of rats after intermittent training. The rats were randomly divided into 4 groups: group A with moderate dose of intermittent exercise, group B with high dose of intermittent exercise, group C with intermittent exercise, group D with blank control group. The treadmill speed was 50 m / min, time 6 minutes, rest for 5 min, then continued to train 3 times a week, 6 days a week, for 4 weeks. The supplementation was done by gavage, the medium dose group was 1g/kg body weight, and the high dose group was 1.5g/kg body weight. The contents of carnosine in gastrocnemius muscle, glycogen content in gastrocnemius muscle and hexokinase activity in gastrocnemius muscle were measured by pre-column derivatization method, colorimetric method and colorimetric method respectively. Glucose content in serum was measured by glucose oxidase method, liver glycogen content was measured by colorimetric method, glucose-6-phosphatase, glucocorticoid receptor and glycogen synthase were measured by real-time fluorescence quantitative PCR. Results the content of carnosine in gastrocnemius in group A was significantly higher than that in group C (p0.05) and group B was significantly higher than that in group C (P 0.01). The activity of hexokinase in gastrocnemius was significantly lower in group A than in group D. The liver glycogen content of rats in group C was significantly higher than that in group D (P 0.01). The mRNA expression of glucose-6-phosphatase in liver of rats in group B and C was significantly higher than that in group D (p0.01A) and the expression of glucocorticoid receptor (Glucocorticoid receptor) in liver of group D (p0.01A). The relative expression of glycogen synthase (mRNA) in liver of group C was significantly higher than that of group B (P 0.01). Conclusion: the relative expression of mRNA of insulin receptor in group A is significantly higher than that in group B (P 0.01). Conclusion: supplement of palanine can increase the expression of glycogen synthase in liver of rats. Promoting intermittent training of the use of blood glucose in rat muscles, Increased liver glycogen decomposition, medium dose effect is better.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：G804.7

【參考文獻】

相關(guān)期刊論文 前10條

1 陳莉明;;肝臟在能量代謝中作用的研究進展——2006年第66屆美國糖尿病學(xué)會(ADA)年會側(cè)記[J];國際內(nèi)分泌代謝雜志;2006年05期

2 熊正英;張新輝;;肌肽與運動能力[J];四川體育科學(xué);2006年04期

3 楊菁;吳國強;白劍;樊淼;劉春娜;王洪新;;鄰苯二甲醛柱前衍生化高效液相色譜法測定組織中肌肽含量[J];中國生化藥物雜志;2009年04期

4 周理紅 ,許梓榮;肌肽的營養(yǎng)研究進展[J];中國飼料;2004年11期

5 陳佩杰;;糖皮質(zhì)激素受體與運動[J];體育科學(xué);2002年03期

6 漆正堂;郭維;張媛;丁樹哲;;不同訓(xùn)練方式對靜息骨骼肌糖酵解能力及線粒體PDK4、CPT-1基因轉(zhuǎn)錄的影響[J];體育科學(xué);2009年03期

7 李潔,李曉新;人體內(nèi)糖與運動能力[J];西北師范大學(xué)學(xué)報(自然科學(xué)版);2004年04期

8 陳岷,葉子堅,李瓊;運動中糖的分解代謝[J];中國臨床康復(fù);2004年33期

9 周蔚;陳立軍;張敏;靳秋月;姚麗;;不同訓(xùn)練負荷條件下SD大鼠運動模型的構(gòu)建與評價[J];中國組織工程研究與臨床康復(fù);2010年37期

10 陳佩杰,許豪文,段子才,蔡斌;糖皮質(zhì)激素受體減少對過度運動疲勞形成的影響[J];中國運動醫(yī)學(xué)雜志;2003年01期

相關(guān)博士學(xué)位論文 前1條

1 李良鳴;運動后糖原恢復(fù)規(guī)律與機制及中藥的干預(yù)研究[D];北京體育大學(xué);2004年

相關(guān)碩士學(xué)位論文 前3條

1 郝玉潔;益氣生津中藥制劑對大鼠游泳運動能力及其糖代謝影響的研究[D];北京體育大學(xué);2010年

2 陽冬輝;拘束應(yīng)激對小鼠糖代謝的影響以及肌肽的改善作用[D];暨南大學(xué);2010年

3 牛美麗;有氧運動聯(lián)合膳食控制對2型糖尿病大鼠骨骼肌糖代謝的影響[D];揚州大學(xué);2010年

，

本文編號：1697550