發(fā)布時間：2019-06-02 18:03

【摘要】：【目的】對山東省刺槐無性系進行遺傳多樣性分析和指紋圖譜構(gòu)建,為刺槐新品種選育、遺傳改良和品種鑒定提供可靠的依據(jù),為中國其他省市刺槐遺傳資源保存、評價與利用提供參考。【方法】采用熒光SSR引物進行PCR擴增,產(chǎn)物經(jīng)毛細管電泳儀進行檢測;利用所得結(jié)果分析49個無性系遺傳多樣性并構(gòu)建其指紋圖譜;采用非加權組平均法(UPGMA)進行基于親緣關系的無性系聚類分析。【結(jié)果】8對SSR引物共檢測到51個等位基因,每個位點的等位基因為2~15個,平均每對引物擴增出6.375個多態(tài)性等位基因。不同位點的PIC值變化范圍很大,在0.092~0.879之間,平均PIC值為0.509 8。使用組間平均數(shù)聯(lián)結(jié)法進行UPGMA聚類分析,結(jié)果表明49份無性系沒有嚴格按照不同區(qū)域聚類到一起,無性系分組與現(xiàn)有栽培區(qū)沒有明顯的規(guī)律。來自臨沂的‘魯刺8’和‘魯刺13’、青島的‘魯刺40’和‘魯刺42’以及日照的‘魯刺10’和‘魯刺86’親緣關系最近。本研究所使用的9對引物中,其中2對Rply109和rops16可以區(qū)分開45份無性系,鑒定率達到91.84%。檢測到22個刺槐無性系在1個或者2個位點得到3個等位基因,表明這些無性系可能是發(fā)生自然變異的多倍體。【結(jié)論】山東省刺槐具有較高的遺傳多樣性。無性系分組與現(xiàn)有栽培區(qū)沒有明顯的規(guī)律。引物Rply109和rops16對49份無性系的分子鑒定率為91.84%,可作為刺槐指紋圖譜構(gòu)建和分子鑒定的高效分子標記。利用SSR標記構(gòu)建的指紋圖譜可為刺槐遺傳資源管理、品種鑒定和知識產(chǎn)權保護奠定堅實的基礎,同時為刺槐的引種和遺傳育種親本選擇提供科學的理論依據(jù)。
[Abstract]:[objective] to analyze the genetic diversity and construct fingerprint of Robinia pseudoacacia clones in Shandong Province, so as to provide reliable basis for breeding, genetic improvement and variety identification of new Robinia pseudoacacia varieties, and to preserve the genetic resources of Robinia pseudoacacia in other provinces and cities of China. The evaluation and utilization provided reference. [methods] fluorescent SSR primers were used for PCR amplification, and the products were detected by capillary electrophoresis. The genetic diversity of 49 clones was analyzed and their fingerprints were constructed. Unweighted group average method (UPGMA) was used to cluster analysis of clones based on genetic relationship. [results] A total of 51 alleles were detected by 8 pairs of SSR primers, and the number of alleles per locus was 2 鈮，

本文編號：2491316