發(fā)布時間：2019-01-05 05:50

【摘要】：利用實驗室前期構(gòu)建的重組大腸桿菌所產(chǎn)磷脂酶A_1(phospholipase A_1,PLA_1)和磷脂酶C (phospholipase C,PLC)進(jìn)行大豆油酶法脫膠研究,探討自主開發(fā)重組酶進(jìn)行酶法脫膠的可行性。以諾維信商品酶Lecitase Ultra~(TM)為對照,研究酶法脫膠反應(yīng)溫度、反應(yīng)pH值、反應(yīng)時間、攪拌速率、復(fù)合磷脂酶添加量工藝參數(shù)對大豆油脫膠的影響,并采用正交試驗對脫膠工藝條件進(jìn)行優(yōu)化。研究結(jié)果表明,大豆油復(fù)合酶法脫膠的最佳工藝參數(shù)為:反應(yīng)溫度45℃、反應(yīng)pH 6.5、反應(yīng)時間3 h、攪拌速率300 r/min、PLA_1和PLC添加量分別為7 940 U/kg和23 130 U/kg。復(fù)合磷脂酶對大豆油脫膠的效果與諾維信商品酶Lecitase Ultra~(TM)基本一致,大豆油磷含量可降至5 mg/kg以下,能夠滿足物理精煉的要求,為進(jìn)一步開發(fā)具有知識產(chǎn)權(quán)的食品級油脂脫膠用酶制劑產(chǎn)品提供了理論依據(jù)。
[Abstract]:The enzymatic degumming of soybean oil was carried out by using phospholipase A1-PLA1 and C (phospholipase, and the feasibility of enzymatic degumming of recombinant Escherichia coli was discussed. The effects of enzymatic degumming reaction temperature, reaction pH value, reaction time, stirring rate and the amount of compound phospholipase on the degumming of soybean oil were studied. Orthogonal test was used to optimize the degumming process conditions. The results showed that the optimum conditions of enzymatic degumming of soybean oil were as follows: reaction temperature 45 鈩，

本文編號：2401362