【摘要】： 研究目的:觀察外力打擊兔頸動脈竇壓力感受器后引起動物死亡,觀察組組織形態(tài)學變化和分析測定心肌組織ANP及血漿兒茶酚胺含量的差異,探討引起死亡的機制。 方法:機械性外力打擊兔頸動脈竇壓力感受器后造成動物神經(jīng)源性休克死亡模型,觀察組織形態(tài)學的變化,運用Western blot檢測心肌組織ANP水平,高效液相-電化學法(HPLC-ECD)檢測兒茶酚胺含量,并與對照組比較。 結果:1、形態(tài)學研究:實驗組死亡動物經(jīng)過檢驗未發(fā)現(xiàn)器質(zhì)性病變,其死亡均由頸動脈竇壓力感受器被打擊后所致。形態(tài)學觀察發(fā)現(xiàn)頸動脈竇外膜損傷表現(xiàn)為灶性片狀出血、神經(jīng)纖維斷裂、神經(jīng)纖維軸漿溶解;心肌組織表現(xiàn)為急性缺血性損傷改變;無明顯肺水腫表現(xiàn)。而存活組亦有部分頸動脈外膜灶性出血、輕度急性心肌缺血性損傷改變。 2、與對照組與存活組比較,死亡組右心耳ANP含量較低。 3、死亡組血漿兒茶酚胺較對照組含量明顯增高(3-10倍)。 結論:頸動脈竇壓力感受器受外界壓力刺激引起死亡的改變有:頸動脈竇外膜損傷表現(xiàn)為灶性片狀出血、神經(jīng)纖維斷裂、神經(jīng)纖維軸漿溶解,急性心肌缺血性損傷改變;心肌組織ANP含量減少;血漿兒茶酚胺含量明顯升高。
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圖片說明：兔頸內(nèi)動脈打擊部位外膜出血HE×20Fig-1haemorrhageofrabbit’sinternalcarotidarteryadventitiabyhit(HE10×20)
[Abstract]:Objective: to observe the animal death caused by external force striking carotid sinus baroreceptor in rabbits, to observe the morphological changes of myocardial tissue and the difference of plasma catecholamine content in myocardial tissue, and to explore the mechanism of death. Methods: the animal model of neurogenic shock was established after mechanical external force hit the carotid sinus baroreceptor in rabbits. The histomorphological changes were observed. The level of ANP in myocardial tissue was measured by Western blot, and the content of catecholamine was detected by high performance liquid phase electrochemical method (HPLC-ECD) and compared with the control group. Results: 1. Morphological study: no organic lesions were found in the dead animals in the experimental group, and the deaths were caused by the attack of carotid sinus baroreceptor. Morphological observation showed that the injury of carotid sinus adventitia showed focal flake hemorrhage, broken nerve fibers and axonal plasma dissolution of nerve fibers, acute ischemic injury in myocardial tissue and no obvious pulmonary edema. In the survival group, some focal hemorrhage of carotid artery and mild acute myocardial ischemic injury were also found. 2. Compared with the control group and survival group, the ANP content of right atrial appendage in the death group was lower than that in the control group and survival group. 3. The content of plasma catecholamine in the dead group was significantly higher than that in the control group (3 鈮，

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