彗星試驗(yàn)檢測(cè)死后DNA降解推斷PMI的參數(shù)選擇研究
發(fā)布時(shí)間:2018-12-15 12:55
【摘要】:目的篩選出彗星試驗(yàn)檢測(cè)死后DNA降解推斷死亡時(shí)間(PM I)的合適彗星參數(shù)。方法大鼠斷頸處死后置于10℃和20℃環(huán)境中44h,每4h取材腦和骨髓組織制成細(xì)胞懸液進(jìn)行彗星電泳,CASP軟件測(cè)量彗星參數(shù),線性回歸分析和比較各參數(shù)與PM I的相關(guān)關(guān)系。結(jié)果死后DNA隨PM I的延長(zhǎng)逐漸降解,彗星頭和尾DNA百分比、尾DNA、O live尾矩、尾面積、尾矩等參數(shù)與PM I呈穩(wěn)定的線性相關(guān)關(guān)系。結(jié)論彗星頭和尾DNA百分比、尾DNA、O live尾矩、尾面積、尾矩等參數(shù)是彗星試驗(yàn)檢測(cè)死后DNA降解推斷PM I的合適參數(shù)。
[Abstract]:Objective to screen the suitable comet parameters for postmortem DNA degradation estimation of (PM I) by comet assay. Methods the rats were sacrificed at 10 鈩,
本文編號(hào):2380666
[Abstract]:Objective to screen the suitable comet parameters for postmortem DNA degradation estimation of (PM I) by comet assay. Methods the rats were sacrificed at 10 鈩,
本文編號(hào):2380666
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