【摘要】：目的探討即早基因c-jun和膠質(zhì)纖維酸性蛋白(GFAP)在彌漫性腦損傷(DBI)中的表達(dá)規(guī)律。方法采用Marmarou方法制作大鼠DBI模型,將65只SD大鼠隨機(jī)分為DBI組及對(duì)照組。用免疫組織化學(xué)技術(shù)(SABC)及圖像分析方法觀察大鼠DBI后15min、30min、1h、3h、6h、12h、24h、2d、3d、4d、6d腦組織內(nèi)c-jun和GFAP表達(dá)規(guī)律,所得數(shù)據(jù)經(jīng)SPSS10.0統(tǒng)計(jì)軟件處理。結(jié)果對(duì)照組大鼠腦組織內(nèi)未見(jiàn)c-jun陽(yáng)性表達(dá),可見(jiàn)少量GFAP表達(dá)。DBI 15min即可在腦組織內(nèi)觀察到c-jun表達(dá),而GFAP蛋白的表達(dá)則在DBI后6h增加。隨著損傷經(jīng)過(guò)時(shí)間的延長(zhǎng),c-jun與GFAP陽(yáng)性細(xì)胞數(shù)及陽(yáng)性表達(dá)范圍逐漸擴(kuò)大,c-jun表達(dá)在DBI后6h達(dá)高峰(P0.01),其后逐漸下降,傷后2d減退;GFAP陽(yáng)性產(chǎn)物則在傷后4d左右達(dá)高峰(P0.01),其后呈下降的趨勢(shì)。結(jié)論應(yīng)用免疫組織化學(xué)方法檢測(cè)c-jun與GFAP可作為診斷DBI的參考指標(biāo),二者在不同時(shí)段內(nèi)表達(dá)所呈現(xiàn)出的時(shí)序性規(guī)律對(duì)損傷時(shí)間的推測(cè)也具有實(shí)際應(yīng)用價(jià)值。
[Abstract]:Objective to investigate the expression of early gene c-jun and glial fibrillary acidic protein (GFAP) (GFAP) in diffuse brain injury (DBI). Methods DBI model was made by Marmarou. 65 SD rats were randomly divided into DBI group and control group. Immunohistochemical (SABC) and image analysis were used to observe the expression of c-jun and GFAP in brain tissue of rats after DBI for 30 minutes, 30 minutes, 1 hour, 1 hour, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 6 days, and the data were processed by SPSS10.0 software. Results there was no positive expression of c-jun in the brain tissue of the control group. A small amount of GFAP expression. DBI 15min could be observed in the brain tissue, while the expression of GFAP protein increased 6 hours after DBI. With the extension of injury time, the number and positive range of c-jun and GFAP increased gradually, and the expression of c-jun peaked at 6h after DBI (P0.01), and then decreased gradually. On the 2nd day after injury, the positive products of GFAP decreased to the peak (P0.01) on the 4th day after injury, and then showed a downward trend. Conclusion the detection of c-jun and GFAP by immunohistochemical method can be used as a reference index for the diagnosis of DBI. The temporal regularity of their expression in different time periods is also valuable for the estimation of injury time.
【作者單位】： 皖南醫(yī)學(xué)院法醫(yī)學(xué)系;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院法醫(yī)學(xué)系;
【分類號(hào)】：D919

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