【摘要】：目的探討即早基因c-jun和膠質(zhì)纖維酸性蛋白(GFAP)在彌漫性腦損傷(DBI)中的表達規(guī)律。方法采用Marmarou方法制作大鼠DBI模型,將65只SD大鼠隨機分為DBI組及對照組。用免疫組織化學(xué)技術(shù)(SABC)及圖像分析方法觀察大鼠DBI后15min、30min、1h、3h、6h、12h、24h、2d、3d、4d、6d腦組織內(nèi)c-jun和GFAP表達規(guī)律,所得數(shù)據(jù)經(jīng)SPSS10.0統(tǒng)計軟件處理。結(jié)果對照組大鼠腦組織內(nèi)未見c-jun陽性表達,可見少量GFAP表達。DBI 15min即可在腦組織內(nèi)觀察到c-jun表達,而GFAP蛋白的表達則在DBI后6h增加。隨著損傷經(jīng)過時間的延長,c-jun與GFAP陽性細胞數(shù)及陽性表達范圍逐漸擴大,c-jun表達在DBI后6h達高峰(P0.01),其后逐漸下降,傷后2d減退;GFAP陽性產(chǎn)物則在傷后4d左右達高峰(P0.01),其后呈下降的趨勢。結(jié)論應(yīng)用免疫組織化學(xué)方法檢測c-jun與GFAP可作為診斷DBI的參考指標,二者在不同時段內(nèi)表達所呈現(xiàn)出的時序性規(guī)律對損傷時間的推測也具有實際應(yīng)用價值。
[Abstract]:Objective to investigate the expression of early gene c-jun and glial fibrillary acidic protein (GFAP) (GFAP) in diffuse brain injury (DBI). Methods DBI model was made by Marmarou. 65 SD rats were randomly divided into DBI group and control group. Immunohistochemical (SABC) and image analysis were used to observe the expression of c-jun and GFAP in brain tissue of rats after DBI for 30 minutes, 30 minutes, 1 hour, 1 hour, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 6 days, and the data were processed by SPSS10.0 software. Results there was no positive expression of c-jun in the brain tissue of the control group. A small amount of GFAP expression. DBI 15min could be observed in the brain tissue, while the expression of GFAP protein increased 6 hours after DBI. With the extension of injury time, the number and positive range of c-jun and GFAP increased gradually, and the expression of c-jun peaked at 6h after DBI (P0.01), and then decreased gradually. On the 2nd day after injury, the positive products of GFAP decreased to the peak (P0.01) on the 4th day after injury, and then showed a downward trend. Conclusion the detection of c-jun and GFAP by immunohistochemical method can be used as a reference index for the diagnosis of DBI. The temporal regularity of their expression in different time periods is also valuable for the estimation of injury time.
【作者單位】： 皖南醫(yī)學(xué)院法醫(yī)學(xué)系;華中科技大學(xué)同濟醫(yī)學(xué)院法醫(yī)學(xué)系;
【分類號】：D919

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