本文選題：法醫(yī)物證學(xué) + 單堿基延伸(SBE)　； 參考：《中國(guó)法醫(yī)學(xué)雜志》2009年04期

【摘要】：目的建立47-p lex SNPs復(fù)合檢測(cè)方法,評(píng)價(jià)其在法醫(yī)學(xué)中的應(yīng)用價(jià)值。方法篩選46個(gè)常染色體SNPs和1個(gè)Y-SNPs,使用2個(gè)檢測(cè)體系分別對(duì)47個(gè)SNPs進(jìn)行單管內(nèi)復(fù)合PCR擴(kuò)增,采用熒光標(biāo)記單堿基延伸法和毛細(xì)管電泳檢測(cè)技術(shù)進(jìn)行分型檢測(cè);并用建立的方法對(duì)260份廣東地區(qū)無關(guān)個(gè)體血樣進(jìn)行47個(gè)SNPs分型。結(jié)果建立的47-p lex SNPs的復(fù)合檢測(cè)體系靈敏度高,種屬特異性好;260名個(gè)體所有SNPs均能準(zhǔn)確分型,群體內(nèi)基因型頻率分布均符合Hardy-W e inberg平衡,累積個(gè)人識(shí)別率大于0.999 9,累積非父排除率為0.999 82,累積偶合率為6.24×10-20。結(jié)論本文47-p lex SNPs復(fù)合檢測(cè)方法能同時(shí)對(duì)47個(gè)SNPs進(jìn)行快速、準(zhǔn)確的檢測(cè),在法醫(yī)學(xué)個(gè)體識(shí)別鑒定中具有良好的應(yīng)用前景。
[Abstract]:Objective to establish a 47-p lex SNPs complex detection method and evaluate its application value in forensic medicine. Methods Forty-six autosomal SNPs and one Y-SNPswere screened. 47 SNPs were amplified by single-tube multiplex PCR using two detection systems. The established method was used for 47 SNPs typing of 260 unrelated individual blood samples in Guangdong area. Results the multiplex detection system of 47-p lex SNPs was highly sensitive and could be accurately typed in 260 individuals with good species-specificity. The frequency distribution of genotypes within the population was in accordance with the Hardy-W inberg equilibrium. The cumulative personal identification rate is greater than 0.999, the cumulative non-paternal exclusion rate is 0.999 82, and the cumulative coupling rate is 6.24 脳 10 ~ (-20). Conclusion 47-p lex SNPs compound detection method can detect 47 SNPs quickly and accurately at the same time, and has a good application prospect in forensic individual identification.
【作者單位】： 廣州市刑事科學(xué)技術(shù)研究所;中山大學(xué)中山醫(yī)學(xué)院法醫(yī)學(xué)系;廣州市公安局南沙分局;
【基金】：國(guó)家十一.五科技支撐計(jì)劃項(xiàng)目(2006BAK07B01) 公安部重點(diǎn)科技攻關(guān)項(xiàng)目(20034421401)
【分類號(hào)】：D919

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