本文選題：法醫(yī)病理學(xué)　切入點：死亡時間　出處：《中國法醫(yī)學(xué)雜志》2009年01期

【摘要】：目的分析死后不同時間大鼠視網(wǎng)膜細(xì)胞核DNA含量變化圖像,探討視網(wǎng)膜細(xì)胞核DNA降解與死亡時間(PM I)的關(guān)系。方法90只成年健康雌性SD大鼠,隨機分成15組,死后0~28h內(nèi)(20℃),每2h提取視網(wǎng)膜細(xì)胞進(jìn)行Feu lgen-Vans染色;采用圖像分析系統(tǒng)檢測視網(wǎng)膜細(xì)胞核的異形指數(shù)(ID)、積分光密度(IOD)和平均光密度(AOD),并運用SPSS 12.0軟件對測量數(shù)據(jù)進(jìn)行線性回歸分析。結(jié)果視網(wǎng)膜細(xì)胞核AOD和IOD隨死亡時間的延長逐漸下降,ID則呈上升趨勢。28h內(nèi)各參數(shù)變化的回歸方程如下:YAOD=-0.009 XAOD+0.590,R2=0.949,YIOD=-0.097 XIOD+18.903,R2=0.968,YID=0.122XID+2.246,R2=0.951。結(jié)論大鼠死后視網(wǎng)膜細(xì)胞核DNA含量隨著死亡時間的延長而呈規(guī)律性降解,并與PM I具有良好的相關(guān)性。
[Abstract]:Objective to analyze the changes of DNA content in retinal nucleus of rats at different postmortem times and to explore the relationship between DNA degradation and death time (Pi).Methods 90 adult healthy female SD rats were randomly divided into 15 groups. Retinal cells were extracted at 20 鈩，

本文編號：1708980