【摘要】：目的:考察黎藥益智仁4種不同極性部位提取物對實驗性腸炎模型小鼠的影響。方法:將益智仁70%乙醇提取物浸膏用水分散后,依次用石油醚、三氯甲烷、乙酸乙酯和正丁醇提取得相應(yīng)部位提取物。將42只小鼠隨機分為造模組(36只)和空白組(6只),造模組大鼠采用2,4,6-三硝基苯磺酸法復(fù)制實驗性腸炎模型。成模后,將造模小鼠隨機分為模型組、陽性組(柳氮磺胺吡啶腸溶片,52 g/kg)和益智仁不同極性部位(石油醚、三氯甲烷、乙酸乙酯和正丁醇部位)提取物組(劑量均為10 g/kg,以生藥計),每組6只,ig給藥,每天1次,連續(xù)9 d,給藥體積為0.2 m L/10 g;空白組和模型組小鼠ig生理鹽水。測定小鼠體質(zhì)量變化,對疾病活動指數(shù)(DAI)進(jìn)行評分,測定結(jié)腸組織中髓過氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)活性,觀察結(jié)腸組織病理變化并進(jìn)行評分。結(jié)果:與空白組比較,模型組小鼠體質(zhì)量下降百分率、DAI評分以及結(jié)腸組織中MPO、MDA活性均顯著升高(P0.05或P0.01),結(jié)腸組織發(fā)生明顯病變。與模型組比較,各給藥組小鼠體質(zhì)量顯著回升,DAI評分顯著降低,MPO活性顯著降低,病理評分顯著降低;陽性組、益智仁正丁醇提取物和乙酸乙酯提取物組小鼠結(jié)腸組織中SOD活性顯著升高,陽性組和益智仁乙酸乙酯提取物組小鼠結(jié)腸組織中MDA活性顯著降低,差異均有統(tǒng)計學(xué)意義(P0.05或P0.01)。結(jié)論:益智仁4種極性部位提取物對實驗性腸炎模型小鼠均有一定改善作用,其中以乙酸乙酯部位和正丁醇部位效果較好。
[Abstract]:Objective: to investigate the effects of four polar extract of Yizhiren on experimental enteritis model mice. Methods: the 70% ethanol extract of Yizhiren was dispersed with water and then extracted with petroleum ether, trichloromethane, ethyl acetate and n-butanol. Forty-two mice were randomly divided into model group (n = 36) and blank group (n = 6). The experimental enteritis model was established by 2,4,6-trinitrobenzenesulfonic acid method. After the model was established, the model mice were randomly divided into model group, positive group (salbutamil enteric-coated tablets, 52 g/kg) and different polar parts of Yizhiren (petroleum ether, trichloromethane, ethyl acetate and n-butanol) (the dose was 10 g 路kg ~ (- 1), measured by crude drug), 6 mice in each group, ig was given once a day for 9 days, and the volume of the drug was 0.2 mL 鈮，

本文編號：2505264