【摘要】：目的:對大鼠顱腦損傷后,于不同時(shí)間點(diǎn)開始介入電針治療,觀察其腦組織中AQP-4的表達(dá)和右側(cè)前后肢回縮力變化與電針介入時(shí)間的關(guān)系,探索電針治療顱腦損傷的最佳時(shí)間窗。方法:選用70只SD大鼠,隨機(jī)分為空白組、模型對照組、電針治療組(電針1組,電針2組),使用e CCI儀器在大鼠顱腦左側(cè)開顱打擊制備TBI(traumatic brain injury)模型。取大鼠"百會、關(guān)元",右側(cè)前肢"曲池、合谷",右側(cè)后肢"足三里、涌泉",電針1組于造模后4 h介入電針治療,電針2組于第8天介入電針治療,每組治療各7 d。(1)造模后8 d、15 d和22 d分別測試并評價(jià)大鼠右側(cè)前后肢肢體回縮力。(2)造模后8 d和15 d取大鼠創(chuàng)傷腦組織,一部分進(jìn)行HE染色及免疫熒光染色,觀察腦組織形態(tài)結(jié)構(gòu)及腦組織中AQP-4蛋白的表達(dá);一部分采用實(shí)時(shí)熒光定量PCR技術(shù)檢測AQP-4mRNA的表達(dá)。結(jié)果:造模后8 d、15 d和22 d三個(gè)時(shí)間點(diǎn)電針1組前后肢肢體回縮力比電針2組、模型組恢復(fù)的好,比空白組稍差。光鏡下,空白組腦組織形態(tài)結(jié)構(gòu)正常;電針治療組比模型組好,電針1組比電針2組好。腦組織中AQP-4蛋白的含量以及mRNA的表達(dá),空白組、模型組、電針治療組各組比較均有統(tǒng)計(jì)學(xué)差異(P0.01),其中電針1組升高最明顯。結(jié)論:電針治療TBI早期介入效果更佳,其機(jī)制可能是通過提高腦組織中AQP-4的表達(dá),減輕腦水腫,達(dá)到保護(hù)神經(jīng)元的作用。
[Abstract]:Objective: to observe the relationship between the expression of AQP-4 in brain tissue and the contractile force of right anterior and posterior limbs and the intervention time of EA at different time points after craniocerebral injury in rats. To explore the best time window for the treatment of craniocerebral injury by electro-acupuncture. Methods: 70 SD rats were randomly divided into blank group, model control group, EA treatment group (EA 1 group, EA 2 group). The TBI (traumatic brain injury) model was established by e CCI instrument in the left craniocerebral craniotomy of rats. The rats were treated with "Baihui, Guanyuan", "Quchi, Hegu" on the right forelimb and "Zusanli, Yongquan" on the right hindlimb. The rats in group 1 were treated with EA 4 hours after modeling, and the two groups were treated with EA on the 8th day. The contractility of the right anterior and posterior limbs of the rats was measured and evaluated on the 8th day, 15th and 22nd day after the model was established. (2) the traumatic brain tissue of the rats was taken 8 and 15 days after the establishment of the model, and the contractile force of the right anterior and posterior limbs was measured and evaluated at the 8th and 15th day after the establishment of the model. In part, HE staining and immunofluorescence staining were used to observe the morphology of brain tissue and the expression of AQP-4 protein in brain tissue. In part, the expression of AQP-4mRNA was detected by real-time fluorescence quantitative PCR. Results: on the 8th day, 15 d and 22 d after modeling, the contractile force of anterior and posterior limbs in group 1 was better than that in group 2, and the recovery in model group was slightly worse than that in blank group. Under light microscope, the morphology of brain tissue in the blank group was normal, the EA treatment group was better than the model group, and the EA 1 group was better than the EA 2 group. The content of AQP-4 protein and the expression of mRNA in brain tissue were significantly different in blank group, model group and electro-acupuncture treatment group (P 0.01), and the increase was most obvious in group 1. Conclusion: the effect of early intervention in the treatment of TBI is better, and the mechanism may be to increase the expression of AQP-4 in brain tissue, reduce brain edema and protect neurons.
【作者單位】： 成都中醫(yī)藥大學(xué)針灸推拿學(xué)院;陜西中醫(yī)藥大學(xué)針灸推拿學(xué)院;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81674088) 陜西省自然科學(xué)基礎(chǔ)研究計(jì)劃項(xiàng)目(2015JZ025)
【分類號】：R245

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