【摘要】：目的篩選藏藥鐮形棘豆醇提物中對(duì)Lewis肺癌荷瘤小鼠具有抑瘤作用的活性部位。方法利用酸堿處理對(duì)鐮形棘豆醇提物進(jìn)行分離,并對(duì)所得堿性部位(AlF)和酸性部位(AcF)進(jìn)行定性、定量分析。建立Lewis肺癌C57BL/6小鼠模型,隨機(jī)分為模型組,環(huán)磷酰胺(CTX)組(20 mg·kg~(-1)),AlF高、低劑量組(180,90 mg·kg~(-1)),AcF高、低劑量組(180,90 mg·kg~(-1)),連續(xù)給藥10 d;通過(guò)測(cè)定小鼠瘤體質(zhì)量、抑瘤率、胸腺指數(shù)、脾指數(shù)來(lái)比較AlF、AcF的抑瘤作用;HE染色,顯微鏡下觀察各組腫瘤細(xì)胞的形態(tài)學(xué)變化。結(jié)果 AcF主要含黃酮類成分,含量為18.9%;AlF主要為生物堿成分,含量為64.1%。AcF高劑量組降低雌性、雄性小鼠瘤體質(zhì)量的作用明顯,與模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01,P0.05),抑瘤率分別為36.3%、32.8%;AlF高劑量組降低雌性小鼠瘤體質(zhì)量的作用明顯,與模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05),抑瘤率為29.7%。與模型組比較,CTX組小鼠的脾臟、胸腺指數(shù)均明顯降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05,P0.01);而AcF、AlF各劑量組與模型組比較,脾臟、胸腺指數(shù)差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。腫瘤組織病理形態(tài)學(xué)觀察顯示AcF、AlF各給藥組腫瘤細(xì)胞出現(xiàn)不同程度的壞死。結(jié)論鐮形棘豆醇提物AlF、AcF均對(duì)Lewis肺癌荷瘤小鼠具有明顯的抗腫瘤作用,黃酮類成分的作用比生物堿略強(qiáng),其抗腫瘤機(jī)制還有待進(jìn)一步研究。
[Abstract]:Objective to screen the active parts of alcohol extract from Tibetan medicine against Lewis lung cancer-bearing mice. Methods the alcohol extract of sickle bean was separated by acid-base treatment, and the (AlF) and (AcF) were analyzed qualitatively and quantitatively. C57BL/6 mice model of Lewis lung cancer was established and randomly divided into model group, cyclophosphamide (CTX) group (20 mg kg~ (- 1), AlF high), low dose group (180, 90 mg kg~ (- 1), AcF), low dose group) (180, 90 mg kg~ (- 1),). Continuous administration for 10 days; The tumor weight, tumor inhibition rate, thymus index and spleen index of mice were measured to compare the antitumor effect of AlF,AcF, and the morphological changes of tumor cells in each group were observed under microscope by HE staining. Results AcF was mainly composed of flavonoids, the content of which was 18.9%. The content of AlF was mainly alkaloid in the high-dose 64.1%.AcF group. The tumor weight of male mice was significantly lower than that of the model group (P0.01, P0.05), and the tumor weight of male mice was significantly lower than that of the model group (P0.01, P0.05). The tumor inhibition rate was 36.3% and 32.8% respectively. Compared with the model group, the high dose of AlF could decrease the tumor weight of female mice significantly (P0.05), and the tumor inhibition rate was 29.7%. Compared with the model group, the index of spleen and thymus in CTX group decreased significantly (P 0.05, P0.01). Compared with the model group, there was no significant difference in spleen and thymus index between each dose group of AcF,AlF and the model group (P0.05). Pathological observation of tumor tissue showed different degree of necrosis of tumor cells in each group of AcF,AlF administration. Conclusion the alcohol extract AlF,AcF from sickle bean has obvious antitumor effect on Lewis lung cancer-bearing mice. The effect of flavonoids is slightly stronger than that of alkaloids, and its anti-tumor mechanism needs to be further studied.
【作者單位】： 南京中醫(yī)藥大學(xué)藥學(xué)院;南京中醫(yī)藥大學(xué)藥用菌與中藥生物技術(shù)研究所;江蘇省中藥炮制重點(diǎn)實(shí)驗(yàn)室國(guó)家中醫(yī)藥管理局中藥炮制標(biāo)準(zhǔn)重點(diǎn)研究室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81473420,30902012) 江蘇高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目(PAPD) 江蘇高校品牌專業(yè)建設(shè)工程資助項(xiàng)目(PPZY2015A070)
【分類號(hào)】：R29

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