發(fā)布時(shí)間：2019-02-15 03:02

【摘要】：目的通過(guò)建立大鼠原發(fā)性痛經(jīng)模型,觀察“秩邊透水道”針?lè)▽?duì)痛經(jīng)大鼠一般行為,扭體次數(shù),血清白介素-1(IL-1)、白介素-6(IL-6)、腫瘤壞死因子-a(TNF-a),血漿β-內(nèi)啡肽(β-EP),下丘腦組織5-羥色胺(5-HT)等多個(gè)指標(biāo)并加以分析,探討“秩邊透水道”針?lè)ㄖ委熢l(fā)性痛經(jīng)的作用機(jī)制,為臨床新技術(shù)的出現(xiàn)提供準(zhǔn)確的實(shí)驗(yàn)依據(jù),以便更好地推廣臨床。方法選用健康雌性SD大鼠40只(230g±15g),造模前實(shí)驗(yàn)室常規(guī)飼養(yǎng)一周使其適應(yīng)環(huán)境,采用隨機(jī)數(shù)字表法將動(dòng)物分為4組:空白組、模型組、針刺組、秩邊組,每組10只。實(shí)驗(yàn)采用催產(chǎn)素致大鼠痛經(jīng)模型:模型組、針刺組及秩邊組大鼠股部皮下注射苯甲酸雌二醇連續(xù)10天,第1、10天0.5mg/只,第2—9天0.2mg/只,末次給藥1 h后,腹腔注射縮宮素2u/只;空白組大鼠每日股部皮下注射同等劑量的生理鹽水。從造模開(kāi)始第一天即進(jìn)行干預(yù),連續(xù)5天,每次20min,空白組、模型組僅抓取固定,針刺組每天造模后針刺關(guān)元、雙側(cè)三陰交穴位,秩邊組于每天造模后即進(jìn)行“秩邊透水道”針?lè)ǜ深A(yù);在造模和治療過(guò)程中觀察大鼠的毛色、飲食、行動(dòng)、體重等一般狀況,記錄腹腔注射縮宮素大鼠30分鐘內(nèi)扭體次數(shù),采用放射免疫法檢測(cè)各組大鼠血清IL-1、IL-6、TNF-a、血漿β-EP、下丘腦組織5-HT的含量,統(tǒng)計(jì)分析相關(guān)指標(biāo),匯總結(jié)果。結(jié)果1、空白組大鼠一般狀況良好,未見(jiàn)明顯異常。模型組大鼠毛色暗淡發(fā)黃,特別是腹部有明顯的發(fā)黃潮濕,食欲降低,動(dòng)作遲緩,體重下降。針刺組與秩邊組大鼠較模型組有很大改善。2、空白組大鼠沒(méi)有出現(xiàn)扭體反應(yīng),模型組、針刺組及秩邊組大鼠全部發(fā)生扭體反應(yīng),模型組大鼠扭體反應(yīng)較明顯,大鼠腹部收縮內(nèi)凹,軀干與后肢伸展,臀部與一側(cè)肢體內(nèi)旋。3、模型組大鼠血漿β-EP含量高于空白組,秩邊組及針刺組的含量均高于模型組,而秩邊組又高于針刺組,均差異顯著(P0.05);模型組與空白組比較,大鼠下丘腦組織中5-HT含量明顯降低,有顯著差異(P0.05);針刺組、秩邊組與模型組比較,大鼠下丘腦組織5-HT含量存在明顯差異(P0.05),秩邊組與針刺組之間沒(méi)有明顯差異;模型組與空白組比較,大鼠血清TNF-a、IL-1、IL-6含量明顯升高,有統(tǒng)計(jì)學(xué)意義(P0.05);針刺組、秩邊組與模型組比較,大鼠血清TNF-a、IL-1、IL-6含量存在明顯差異(P0.05),秩邊組與針刺組之間沒(méi)有明顯差異。結(jié)論原發(fā)性痛經(jīng)大鼠實(shí)驗(yàn)?zāi)Ｐ椭苽涑晒?“秩邊透水道”針?lè)苊黠@改善原發(fā)性痛經(jīng)大鼠模型的一般狀況,降低扭體次數(shù),提高大鼠下丘腦組織5-HT及血漿β-EP的含量,降低血清IL-1、IL-6、TNF-a的含量,初步闡明了其治療原發(fā)性痛經(jīng)的作用機(jī)制,有利于基礎(chǔ)研究與臨床應(yīng)用的銜接,為新技術(shù)的出現(xiàn)提供實(shí)驗(yàn)支持。
[Abstract]:Objective to observe the general behavior, writhing times, serum interleukin-1 (IL-1) and interleukin-6 (IL-6) in rats with dysmenorrhea by establishing a rat model of primary dysmenorrhea. Tumor necrosis factor-a (TNF-a), plasma 尾 -endorphin (尾 -endorphin) and 5-hydroxytryptamine (5-HT) in hypothalamus were analyzed. To provide accurate experimental basis for the emergence of new clinical technology, in order to better promote clinical. Methods Forty healthy female SD rats (230g 鹵15g) were randomly divided into four groups: blank group, model group, acupuncture group and rank side group. The model of dysmenorrhea induced by oxytocin: the rats in the model group, acupuncture group and rank side group were injected estradiol benzoate subcutaneously into the femoris for 10 days, only 0.5mg/ on the 1st day, 0.2mg/ on the 2nd to 9th day, and 1 hour after the last administration of estradiol benzoate. Intraperitoneal injection of oxytocin 2u/; Rats in the blank group were subcutaneously injected with the same dose of normal saline daily. Intervention was carried out from the first day of modeling for 5 days each time for 20 minutes. The blank group and the model group only grasped fixation. The acupuncture group needled Guanyuan after modeling every day, and the acupoints of Sanyinjiao on both sides were punctured. In the rank side group, the needle intervention of "rank edge permeating waterway" was carried out immediately after making model every day. In the course of modeling and treatment, we observed the general condition of color, diet, exercise and body weight of rats, recorded the times of writhing in 30 minutes after intraperitoneal injection of oxytocin, and detected the serum IL-1,IL-6, of rats by radioimmunoassay. The content of 5-HT in hypothalamus tissue of plasma 尾-EP, in TNF-a, was analyzed statistically and the results were summarized. Results 1. The normal condition of the blank group was good and no obvious abnormality was found. In the model group, the hair color was dim and yellowish, especially in the abdomen with obvious yellowish humidity, decreased appetite, slow movement and weight loss. Compared with the model group, the acupuncture group and the rank side group showed significant improvement. 2. There was no writhing reaction in the blank group, all the rats in the model group, the acupuncture group and the rank side group had writhing reaction, and the writhing reaction was more obvious in the model group. The contents of plasma 尾-EP in the model group were higher than those in the blank group, the contents of 尾-EP in the rank side group and acupuncture group were higher than those in the model group, and the rank side group was higher than that in the acupuncture group, and the content of 尾-EP in the rank side group was higher than that in the acupuncture group. The difference was significant (P0.05). Compared with the blank group the content of 5-HT in the hypothalamus of the model group was significantly lower than that of the blank group (P0.05). In acupuncture group, rank side group and model group, the content of 5-HT in hypothalamus was significantly different (P0.05), but there was no significant difference between rank side group and acupuncture group. Compared with the blank group, the serum TNF-a,IL-1,IL-6 content in the model group was significantly higher than that in the blank group (P0.05). Compared with the model group, the serum TNF-a,IL-1,IL-6 content in acupuncture group, rank edge group and model group was significantly different (P0.05), but there was no significant difference between the rank side group and acupuncture group. Conclusion the experimental model of primary dysmenorrhea in rats is successful. The acupuncture method of "rank side through waterway" could obviously improve the general condition of primary dysmenorrhea rat model, decrease the number of writhing, increase the content of 5-HT and plasma 尾-EP in hypothalamus, and decrease the content of serum IL-1,IL-6,TNF-a. The mechanism of treatment of primary dysmenorrhea is elucidated preliminarily, which is beneficial to the connection between basic research and clinical application, and provides experimental support for the emergence of new technology.
【學(xué)位授予單位】：山西中醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R245

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李文紅;;中藥治療氣滯血瘀型痛經(jīng)的觀察及護(hù)理[J];云南中醫(yī)中藥雜志;2016年03期

2 吳青燕;;活血溫經(jīng)湯治療原發(fā)性痛經(jīng)51例[J];中國(guó)民間療法;2015年11期

3 潘麗娟;;淺析圣愈湯治療氣血虛弱型痛經(jīng)的臨床效果[J];山東醫(yī)學(xué)高等專(zhuān)科學(xué)校學(xué)報(bào);2014年01期

4 周蓉芳;常宗煥;;自擬益氣養(yǎng)血止痛湯治療氣血虛弱型痛經(jīng)123例[J];內(nèi)蒙古中醫(yī)藥;2012年18期

5 李龍?bào)J;;原發(fā)性痛經(jīng)辨治探微[J];陜西中醫(yī);2012年01期

6 白石;袁麗江;;芬必得治療原發(fā)性痛經(jīng)臨床療效觀察[J];中國(guó)社區(qū)醫(yī)師(醫(yī)學(xué)專(zhuān)業(yè));2011年18期

7 劉麗敏;;鎮(zhèn)痛方治療氣血瘀滯型原發(fā)性痛經(jīng)50例療效觀察[J];河北中醫(yī);2011年05期

8 楊海俠;陶桂梅;;消結(jié)安膠囊治療氣滯血瘀型痛經(jīng)96例[J];吉林醫(yī)學(xué);2010年33期

9 晏麗;;維生素E治療原發(fā)性痛經(jīng)的臨床觀察[J];醫(yī)學(xué)信息(中旬刊);2010年11期

10 馬宗國(guó);江南;邵欣;袁野;曹定知;張繼成;;針刺三陰交 關(guān)元穴調(diào)經(jīng)止痛治療原發(fā)性痛經(jīng)的實(shí)驗(yàn)研究[J];四川中醫(yī);2010年08期

相關(guān)博士學(xué)位論文 前2條

1 樊純?nèi)A;隔姜灸治療寒凝血瘀型痛經(jīng)的臨床研究[D];廣州中醫(yī)藥大學(xué);2014年

2 楊靜宜;原發(fā)性痛經(jīng)的文獻(xiàn)及臨床研究[D];南京中醫(yī)藥大學(xué);2006年

相關(guān)碩士學(xué)位論文 前2條

1 周亞紅;活血化瘀中藥保留灌腸對(duì)子宮內(nèi)膜異位癥腹腔液CA125及IL-6的影響的臨床研究[D];南京中醫(yī)藥大學(xué);2014年

2 廖彥晴;當(dāng)歸芍藥散治療原發(fā)性痛經(jīng)的療效觀察[D];廣州中醫(yī)藥大學(xué);2012年

，

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