122味維吾爾藥體外抗真菌作用
發(fā)布時(shí)間：2019-02-11 12:27
【摘要】：目的測(cè)定122味維吾爾藥的體外抗真菌活性,以期找到活性較好的抗真菌藥物。方法用75%乙醇超聲提取維吾爾藥;美國(guó)臨床實(shí)驗(yàn)室標(biāo)準(zhǔn)化協(xié)會(huì)(CLSI)推薦的M38-A2和M27-A3微量液基稀釋法進(jìn)行抗深部真菌活性篩選;棋盤微量液基法考察維吾爾藥與氟康唑協(xié)同抗耐藥白假絲酵母菌的作用;磷鉬鎢酸-干酪素法測(cè)定鞣質(zhì)含量,并比較不同溶劑提取部位抑菌活性與鞣質(zhì)含量的關(guān)系。結(jié)果和結(jié)論在122味維吾爾藥活性測(cè)定中,在2 000μg/m L測(cè)定濃度下,對(duì)新生隱球菌有抑制作用的有黃訶子皮(Terminalia chebula Retz.)、毛訶子(Terminalia bellerica Roxb.)等21味,對(duì)白假絲酵母菌有抑制作用的有黃訶子皮、毛訶子等15味,對(duì)煙曲霉僅姜黃(Curcuma longa L.)有效。姜黃抗菌譜最廣,且對(duì)深部真菌及淺部真菌均有較好的抑制作用。9味維吾爾藥與氟康唑合用對(duì)耐藥白假絲酵母菌有較好協(xié)同抗真菌作用;黃訶子皮、毛訶子、巖白菜(Bergenia purpurascens Engl.)、阿拉伯膠樹汁(Acacia senegal Willd)、鞣樹果(Rhus coriaria L.)這5味活性較好藥材的抗真菌活性部位集中在乙酸乙酯部位,且不同溶劑提取部位的最低抑菌濃度與鞣質(zhì)含量呈正相關(guān)。
[Abstract]:Objective to determine the antifungal activity of 122 Uygur drugs in vitro. Methods Uygur drugs were extracted by 75% ethanol ultrasound, and the antifungal activity of M38-A2 and M27-A3 were screened by M38-A2 and M27-A3 microdilution method recommended by (CLSI) of American Clinical Laboratory Standardization Association. The effect of Uygur medicine and fluconazole on the resistance to drug-resistant Candida albicans was investigated by chessboard microliquid method, and the content of tannin was determined by phosphomolybdenum tungstic acid-casein method, and the relationship between bacteriostatic activity and tannin content in different solvent extracts was compared. Results and conclusion in the determination of the activity of 122 Uygur drugs and at the concentration of 2 000 渭 g / mL, the (Terminalia bellerica Roxb.) of (Terminalia chebula Retz.), has inhibitory effect on Cryptococcus neoformans. There are 15 flavours of Cortex xanthophylla and Fructus chebula with inhibitory effect on Candida albicans, and only (Curcuma longa L.) of turmeric against Aspergillus fumigatus. Effective. The antibacterial spectrum of turmeric was the most extensive and had a good inhibitory effect on deep fungi and superficial fungi. 9 Uygur drugs combined with fluconazole had a good synergistic antifungal effect on drug-resistant Candida albicans. (Bergenia purpurascens Engl.), (Bergenia purpurascens Engl.), Arabian gum tree juice (Acacia senegal Willd), tanned tree fruit (Rhus coriaria L.) The antifungal active sites of these five medicinal herbs were concentrated in ethyl acetate, and the minimum inhibitory concentration of different solvent extracts was positively correlated with the content of tannin.
【作者單位】： 第二軍醫(yī)大學(xué)藥學(xué)院新藥研究中心;新疆維吾爾自治區(qū)中藥民族藥研究所;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81470158) 國(guó)家藥品(維藥)標(biāo)準(zhǔn)提高項(xiàng)目(290)~~
【分類號(hào)】：R29
本文編號(hào)：2419733
[Abstract]:Objective to determine the antifungal activity of 122 Uygur drugs in vitro. Methods Uygur drugs were extracted by 75% ethanol ultrasound, and the antifungal activity of M38-A2 and M27-A3 were screened by M38-A2 and M27-A3 microdilution method recommended by (CLSI) of American Clinical Laboratory Standardization Association. The effect of Uygur medicine and fluconazole on the resistance to drug-resistant Candida albicans was investigated by chessboard microliquid method, and the content of tannin was determined by phosphomolybdenum tungstic acid-casein method, and the relationship between bacteriostatic activity and tannin content in different solvent extracts was compared. Results and conclusion in the determination of the activity of 122 Uygur drugs and at the concentration of 2 000 渭 g / mL, the (Terminalia bellerica Roxb.) of (Terminalia chebula Retz.), has inhibitory effect on Cryptococcus neoformans. There are 15 flavours of Cortex xanthophylla and Fructus chebula with inhibitory effect on Candida albicans, and only (Curcuma longa L.) of turmeric against Aspergillus fumigatus. Effective. The antibacterial spectrum of turmeric was the most extensive and had a good inhibitory effect on deep fungi and superficial fungi. 9 Uygur drugs combined with fluconazole had a good synergistic antifungal effect on drug-resistant Candida albicans. (Bergenia purpurascens Engl.), (Bergenia purpurascens Engl.), Arabian gum tree juice (Acacia senegal Willd), tanned tree fruit (Rhus coriaria L.) The antifungal active sites of these five medicinal herbs were concentrated in ethyl acetate, and the minimum inhibitory concentration of different solvent extracts was positively correlated with the content of tannin.
【作者單位】： 第二軍醫(yī)大學(xué)藥學(xué)院新藥研究中心;新疆維吾爾自治區(qū)中藥民族藥研究所;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81470158) 國(guó)家藥品(維藥)標(biāo)準(zhǔn)提高項(xiàng)目(290)~~
【分類號(hào)】：R29
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1 周汛;李桂明;;23種中藥對(duì)皮膚淺部真菌感染的體外抗真菌活性研究[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2008年11期
2 ;[J];;年期
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