【摘要】：目的:觀察巨刺對(duì)MCAO/R模型大鼠大腦皮質(zhì)缺血區(qū)VEGF和Ang-1表達(dá)水平的影響,探討巨刺治療急性腦缺血再灌注損傷大鼠的作用機(jī)制。方法:將100只雄性SD大鼠隨機(jī)分為空白組、假手術(shù)組、模型組、巨刺組(即健側(cè)針刺組)、患側(cè)針刺組,各20只,各組再分別分為3d亞組和14d亞組,每組各10只。采用Zea Longa線栓法制備MCAO/R模型。2小時(shí)后再灌注。24h后分別進(jìn)行治療。巨刺組針刺“人中”、“百會(huì)”及電針治療健側(cè)肢體的曲池、內(nèi)關(guān)、足三里、三陰交;患側(cè)針刺組針刺“人中”、“百會(huì)”及電針治療患側(cè)肢體的曲池、內(nèi)關(guān)、足三里、三陰交。造模后和療程結(jié)束后予神經(jīng)功能評(píng)分,觀察大鼠缺血腦組織結(jié)構(gòu);采用免疫組化法(S-ABC法)測(cè)定缺血區(qū)大腦皮質(zhì)VEGF和Ang-1表達(dá)情況。結(jié)果:1.3d和14d亞組中,造模后各組均出現(xiàn)不同程度神經(jīng)功能缺損癥狀,提示模型制備成功.與模型組相比,巨刺組、患側(cè)針刺組神經(jīng)功能均無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)。治療3天后,巨刺組、患側(cè)針刺組分別與模型組比較,神經(jīng)功能均得到改善,而巨刺組、患側(cè)針刺組比較無(wú)統(tǒng)計(jì)學(xué)差異。(P0.05)巨刺組、患側(cè)針刺組治療前后各自比較差異均有意義(P0.05)。治療14天后,巨刺組、患側(cè)針刺組與模型組比較神經(jīng)功能得到改善(P0.05),巨刺組較患側(cè)針刺組神經(jīng)功能改善較明顯(P0.05)。巨刺、患側(cè)針刺組治療14天后與治療前比較,神經(jīng)功能改善均具有統(tǒng)計(jì)學(xué)意義(P0.01)。2.與模型組相比,針刺治療后大鼠缺血區(qū)腦組織損傷程度均明顯改善,其中巨刺組較患側(cè)針刺組改善明顯,且14d亞組較3d亞組缺血腦組織改善明顯。3.巨刺組、患側(cè)針刺組VEGF和Ang-1的表達(dá)均增多(P0.05);而巨刺組VEGF和Ang-1的表達(dá)均較患側(cè)針刺組增多明顯(P0.05)。結(jié)論:巨刺具有改善急性缺血再灌注損傷大鼠缺血區(qū)腦組織損傷程度,改善腦缺血大鼠神經(jīng)功能缺損,其機(jī)制可能與顯著上調(diào)腦組織VEGF和Ang-1表達(dá)水平有關(guān)。
[Abstract]:Aim: to observe the effects of giant prickles on the expression of VEGF and Ang-1 in cerebral cortex ischemia area of MCAO/R model rats and to explore the mechanism of giant prickles in the treatment of acute cerebral ischemia-reperfusion injury in rats. Methods: one hundred male SD rats were randomly divided into three groups: blank group, sham operation group, model group, giant prick group (healthy side acupuncture group) and affected side acupuncture group (20 rats). Each group was subdivided into 3 d subgroup and 14 d subgroup with 10 rats in each group. The model of MCAO/R was established by Zea Longa thread embolization method. 2 hours after reperfusion, 24 hours after treatment, respectively. Giant stab group acupuncture "human middle", "Baihui" and electroacupuncture treatment of the healthy side of the limb qu Chi, Neiguan, Zusanli, Sanyinjiao; The affected side acupuncture group acupuncture "human middle", "Baihui" and electroacupuncture treatment of the affected side limb qu Chi, Neiguan, Zusanli, Sanyinjiao. After the model was made and the course of treatment was finished, the neural function score was used to observe the structure of the ischemic brain, and the expression of VEGF and Ang-1 in the cerebral cortex of the ischemic area was determined by immunohistochemical method (S-ABC). Results: 1. In the subgroup of day 3 and day 14, the symptoms of different degrees of nerve function defect appeared in each group after the model was made, which indicated that the model was successfully prepared. Compared with the model group, there was no significant difference in nerve function between the giant prick group and the affected side acupuncture group (P0.05). After 3 days of treatment, the nerve function of the giant prick group and the affected side acupuncture group were all improved compared with the model group, but there was no statistical difference between the giant prick group and the affected side acupuncture group. (P0.05) the nerve function of the giant needling group was significantly higher than that of the model group. There were significant differences between the two groups before and after treatment (P0.05). After 14 days of treatment, the nerve function of the giant prick group and the affected side acupuncture group was improved compared with the model group (P0.05), and the nerve function of the giant prick group was significantly improved than that of the affected side acupuncture group (P0.05). After 14 days of treatment, the improvement of nerve function was statistically significant (P0.01). Compared with the model group, the degree of cerebral tissue injury in ischemic area of rats after acupuncture treatment was significantly improved, in which the giant prick group was significantly improved than the affected side acupuncture group, and the 14 d subgroup was significantly improved than that of 3 d subgroup. The expression of VEGF and Ang-1 in the giant prick group was higher than that in the affected side acupuncture group (P0.05), while the expression of VEGF and Ang-1 in the giant prick group was significantly higher than that in the affected side acupuncture group (P0.05). Conclusion: Giant prickly can improve the degree of cerebral tissue injury in rats with acute ischemia-reperfusion injury and improve the neural function defect in ischemic rats. The mechanism may be related to the up-regulation of the expression of VEGF and Ang-1 in the brain tissue of rats with acute ischemia-reperfusion injury.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

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