【摘要】：目的:觀察電針"委中"穴對(duì)布比卡因(bupivacaine,BPVC)致大鼠腰多裂肌損傷后組織形態(tài)學(xué)及磷酸肌酸激酶(CK)、白介素17(IL-17)表達(dá)水平的影響。方法:將32只雄性SD大鼠隨機(jī)分為對(duì)照組、模型組、電針委中組和電針腎俞組,每組8只。模型組、電針委中組和電針腎俞組采用0.5%BPVC肌內(nèi)注射制備多裂肌損傷模型;對(duì)照組采用同樣的方法注射0.9%Na Cl溶液。電針委中組、電針腎俞穴分別電針"委中"與"腎俞"穴,選用疏密波,頻率2 Hz/10 Hz,電流強(qiáng)度1~2 m A,持續(xù)20 min;對(duì)照組與模型組不進(jìn)行針刺干預(yù)。電針干預(yù)14 d后,通過蘇木精-伊紅(HE)染色和馬松三色染色法(Masson)觀察多裂肌炎細(xì)胞計(jì)數(shù)、瘢痕面積和肌纖維橫截面積的變化。通過酶聯(lián)免疫吸附法(ELISA)檢測(cè)血清CK活性和IL-17的含量,并用免疫組化法檢測(cè)多裂肌損傷部位的IL-17表達(dá)。結(jié)果:干預(yù)后,模型組、電針委中組和電針腎俞組的炎細(xì)胞計(jì)數(shù)、瘢痕面積明顯高于對(duì)照組(均P0.01),肌纖維橫截面積明顯減少(均P0.01);電針委中組和電針腎俞組炎細(xì)胞計(jì)數(shù)、瘢痕面積均少于模型組(均P0.01),肌纖維橫截面積大于模型組(P0.01,P0.05)。干預(yù)后,模型組、電針委中組和電針腎俞組多裂肌損傷局部的IL-17表達(dá)、血清IL-17含量及CK活性均明顯高于對(duì)照組(均P0.01);電針委中組和電針腎俞組多裂肌中IL-17的表達(dá)、血清IL-17含量及CK活性均低于模型組(P0.01,P0.05);與電針腎俞組比較,電針委中組下調(diào)IL-17的趨勢(shì)更明顯(P0.01)。結(jié)論:電針"委中"穴可通過下調(diào)血清CK和白介素-17的過度表達(dá),減輕炎性反應(yīng),促進(jìn)多裂肌的良性修復(fù)。
[Abstract]:Aim: to observe the effect of electroacupuncture on histomorphology and expression of (CK), interleukin 17 (IL-17) of phosphocreatine kinase (CK),) after injury of lumbar polyfissure muscle induced by bupivacaine (bupivacaine,BPVC) in rats. Methods: 32 male SD rats were randomly divided into control group, model group, electroacupuncture group and Shenshu group with 8 rats in each group. Model group, electroacupuncture group and Shenshu group were injected intramuscularly with 0.5%BPVC to make the injury model of polyfissure muscle, while the control group were injected with 0.9%Na Cl solution in the same way. Electroacupuncture group and Shenshu point were treated with "Wei Zhong" and "Shen Shu", respectively, with the frequency of 2 Hz/10 Hz, and the frequency of 2 Hz/10 Hz,. The control group and the model group were not treated with acupuncture for 20 min;. After 14 days of electroacupuncture intervention, the changes of cell count, scar area and cross sectional area of muscle fiber were observed by hematoxylin eosin (HE) staining and Ma Song trichromatic (Masson) staining. The activity of CK and the content of IL-17 in serum were detected by (ELISA), and the expression of IL-17 was detected by immunohistochemistry. Results: after intervention, the inflammatory cell count, scar area and muscle fiber cross-sectional area in model group, electroacupuncture medium group and electroacupuncture Shenshu group were significantly higher than those in control group (P0.01). The number of cells and scar area in EA group and EA group were less than those in model group (P0.01), and the cross sectional area of muscle fiber was larger than that in model group (P0.01P 0.05). After intervention, the expression of IL-17, the content of serum IL-17 and the activity of CK in model group, electroacupuncture group and Shenshu group were significantly higher than those in control group (P0.01). The expression of IL-17, the content of serum IL-17 and the activity of CK were lower in the electroacupuncture group and Shenshu group than those in the model group (P 0.01 P 0.05). Compared with the Shenshu group, the down-regulation of IL-17 was more obvious in the electroacupuncture group (P0.01). Conclusion: electroacupuncture can attenuate inflammatory reaction and promote benign repair of polyfissure muscle by down-regulating the overexpression of serum CK and interleukin-17.
【作者單位】： 北京中醫(yī)藥大學(xué)針灸推拿學(xué)院;廣東省第二中醫(yī)院針灸康復(fù)科;北京大學(xué)第三醫(yī)院中醫(yī)科;
【基金】：國家自然科學(xué)基金面上項(xiàng)目:81574052
【分類號(hào)】：R245.97

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