針刺對去勢雌兔干眼癥模型眼表粘蛋白MUC5AC、MUC19表達(dá)的影響
發(fā)布時(shí)間：2018-12-12 04:24
【摘要】：目的研究針刺對更年期干眼癥模型去勢雌兔眼表蛋白MUC5AC和MUC19的表達(dá)影響,為更年期干眼癥治療提供依據(jù)。方法選擇健康成年雌兔45只,將其隨機(jī)分為對照組、模型組、針刺組(每組15只),模型組和針刺組行雙側(cè)卵巢切除建立干眼模型,2周后對針刺組采取針刺治療,分別于去勢前、2周后、3周后、6周后行Schirmer試驗(yàn)、淚膜破裂時(shí)間檢測、熒光染色評分、虎紅染色評分、免疫組化染色檢測MUC5AC的MUC19表達(dá)水平。結(jié)果 2周和3周時(shí)模型組和針刺組Shirmer值、BUT時(shí)間、熒光染色評分、虎紅染色評分值與對照組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05);3周時(shí)針刺組和模型組的Shirmer值和虎紅染色評分差異有統(tǒng)計(jì)學(xué)意義(P0.05);6周時(shí)模型組的Shirmer值、BUT時(shí)間、熒光染色評分、虎紅染色評分值與對照組和針刺組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。實(shí)驗(yàn)3周后針刺組MUC5AC和MUC19表達(dá)水平較模型組顯著升高(P0.05),與對照組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05);6周后針刺組黏蛋白表達(dá)水平繼續(xù)升高,明顯高于模型組(P0.05)。結(jié)論針刺治療可提高眼表黏蛋白的表達(dá),提示其在干眼癥治療中具有應(yīng)用價(jià)值。
[Abstract]:Objective to study the effect of acupuncture on the expression of surface protein MUC5AC and MUC19 in ovariectomized female rabbits, and to provide evidence for the treatment of menopausal xerophthalmia. Methods Forty-five healthy adult female rabbits were randomly divided into control group, model group, acupuncture group (15 rabbits in each group), model group and acupuncture group with bilateral ovariectomies to establish dry eye model, and acupuncture group was treated with acupuncture 2 weeks later. Schirmer test was performed before castration, 2 weeks later, 3 weeks later and 6 weeks later. Tear film rupture time, fluorescent staining score, tiger red staining score and MUC19 expression level of MUC5AC were detected by immunohistochemical staining. Results the Shirmer, BUT time, fluorescent staining score and tiger red staining score of model group and acupuncture group were significantly different from those of control group at 2 and 3 weeks (P0.05). There were significant differences in Shirmer value and tiger red staining score between the acupuncture group and the model group at 3 weeks (P0.05). At 6 weeks, the Shirmer value, BUT time, fluorescent staining score and tiger red staining score of the model group were significantly different from those of the control group and the acupuncture group (P0.05). After 3 weeks, the expression of MUC5AC and MUC19 in the acupuncture group was significantly higher than that in the model group (P0.05), and the difference was statistically significant compared with the control group (P0.05). After 6 weeks, the expression of mucin in acupuncture group continued to increase, which was significantly higher than that in model group (P0.05). Conclusion Acupuncture therapy can improve the expression of mucin on ocular surface, suggesting that acupuncture can be used in the treatment of xerophthalmia.
【作者單位】： 浙江省中醫(yī)院眼科;
【基金】：2015年浙江省中醫(yī)藥適宜技術(shù)推廣項(xiàng)目(2015-ZA086)
【分類號】：R245;R-332
，
本文編號：2373899
[Abstract]:Objective to study the effect of acupuncture on the expression of surface protein MUC5AC and MUC19 in ovariectomized female rabbits, and to provide evidence for the treatment of menopausal xerophthalmia. Methods Forty-five healthy adult female rabbits were randomly divided into control group, model group, acupuncture group (15 rabbits in each group), model group and acupuncture group with bilateral ovariectomies to establish dry eye model, and acupuncture group was treated with acupuncture 2 weeks later. Schirmer test was performed before castration, 2 weeks later, 3 weeks later and 6 weeks later. Tear film rupture time, fluorescent staining score, tiger red staining score and MUC19 expression level of MUC5AC were detected by immunohistochemical staining. Results the Shirmer, BUT time, fluorescent staining score and tiger red staining score of model group and acupuncture group were significantly different from those of control group at 2 and 3 weeks (P0.05). There were significant differences in Shirmer value and tiger red staining score between the acupuncture group and the model group at 3 weeks (P0.05). At 6 weeks, the Shirmer value, BUT time, fluorescent staining score and tiger red staining score of the model group were significantly different from those of the control group and the acupuncture group (P0.05). After 3 weeks, the expression of MUC5AC and MUC19 in the acupuncture group was significantly higher than that in the model group (P0.05), and the difference was statistically significant compared with the control group (P0.05). After 6 weeks, the expression of mucin in acupuncture group continued to increase, which was significantly higher than that in model group (P0.05). Conclusion Acupuncture therapy can improve the expression of mucin on ocular surface, suggesting that acupuncture can be used in the treatment of xerophthalmia.
【作者單位】： 浙江省中醫(yī)院眼科;
【基金】：2015年浙江省中醫(yī)藥適宜技術(shù)推廣項(xiàng)目(2015-ZA086)
【分類號】：R245;R-332
，
本文編號：2373899
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