肺與大腸俞募配穴電針改善急性胰腺炎大鼠胃腸動力—減輕肺損傷的機理
發(fā)布時間：2018-12-07 20:20
【摘要】：背景：急性肺損傷(acute lung injury, ALI)是重癥急性胰腺炎(severe acute pancreatitis, SAP)常見的并發(fā)癥,其發(fā)生與胃腸功能障礙密切相關(guān)。既往研究已經(jīng)證實,針刺可通過調(diào)節(jié)胃腸激素水平,改善SAP大鼠胃腸功能障礙,以減輕肺部炎癥損傷。但肺與大腸兩經(jīng)俞募配穴電針治療急性胰腺炎(acute pancreatitis, AP)相關(guān)性ALI的機理及最佳針刺配穴不清。目的：基于中醫(yī)理論“肺與大腸相表里”,以SAP最常發(fā)生的腸道功能障礙和肺損傷為切入點,選擇肺與大腸的俞募配穴來研究俞募配穴電針改善胃腸功能、防治ALl的機理,以探索俞募配穴治療SAP的療效原理和最佳穴位配伍。方法：40只雄性Sprague-Dawley (SD)大鼠,隨機分為空白組、模型組、俞穴電針組、募穴電針組和俞募配穴電針組(每組8只)。電針治療組及模型組均采用3.5%�；悄懰徕c逆行胰膽管微量泵泵入法制備SAP模型,造模成功2h后開始給予電針治療,24h內(nèi)給予三個電針治療組電針治療各3次,兩次電針治療間隔時間為7h,留針時間為40min(俞穴電針組：雙側(cè)肺俞與大腸俞留針40min；募穴電針組：雙側(cè)中府穴與天樞穴留針40min；俞募電針組：雙側(cè)肺俞與大腸俞留針20min,雙側(cè)中府穴與天樞穴留針20mmin)�？兆越M和模型組給予同等時間的捆綁處理。造模后36h采集標(biāo)本：心血2m1,取上清液,用ELISA測血清淀粉酶(amylase, AMY)、膽囊收縮素(cholecystokinin, CCK)、血管活性腸肽(vasoactive intestinal peptide, VIP)、胃動素(motilin, MTL)和二胺氧化酶(Diamine Oxidase, DAO);取肺、胰腺、大腸組織測炎癥損傷因子,用ELISA測組織髓過氧化物酶(myeloperxidase, MPO)和丙二醛(malondialdehyde, MDA),做病理切片,行病理評分。結(jié)果：電針治療后,三個電針治療組肺、胰腺、大腸MPO、MDA和血清AMY、VIP均較模型組明顯降低(P0.05),血清MTL明顯升高(P0.05),且俞募配穴電針組大鼠肺、胰腺、大腸病理評分和血清CCK、DAO較模型組明顯降低(P0.05)。在血清VIP、MTL,肺及大腸組織MPO、MDA等方面,三個電針治療組組間兩兩比較,有統(tǒng)計學(xué)差異,俞募配穴電針組優(yōu)于單純的俞穴電針組或募穴電針組。結(jié)論：肺與大腸兩經(jīng)俞募穴電針都能通過調(diào)節(jié)胃腸激素、促進胃腸運動,改善胃腸功能障礙以減輕肺損傷,且俞募配穴電針療效更優(yōu)。
[Abstract]:Background: acute lung injury (acute lung injury, ALI) is a common complication of severe acute pancreatitis (severe acute pancreatitis, SAP). Previous studies have shown that acupuncture can alleviate lung inflammation by regulating gastrointestinal hormone levels and improving gastrointestinal dysfunction in SAP rats. However, the mechanism of electroacupuncture on (acute pancreatitis, AP) associated ALI and the optimal acupuncture of acupoints in the treatment of acute pancreatitis were not clear, but the two meridians of lung and large intestine were combined with acupoint electroacupuncture. Objective: based on the theory of "lung and large intestine" and taking the intestinal dysfunction and lung injury that occur most frequently in SAP as the starting point, the author chose the point of Shu-fu-pairing of lung and large intestine to study the mechanism of improving gastrointestinal function and preventing and treating ALl by electroacupuncture of Yu-fu-pin. Objective: to explore the principle of therapeutic effect and the best combination of acupoints in the treatment of SAP. Methods: forty male Sprague-Dawley (SD) rats were randomly divided into three groups: blank group, model group, electroacupuncture group at Shu point, electroacupuncture group at Jun acupoint and electroacupuncture group at Shufu point (8 rats in each group). The SAP model was made by injecting 3.5% sodium taurocholate retrograde pump into the pancreaticobiliary duct in both the electroacupuncture group and the model group. Two hours after the establishment of the model, electroacupuncture was given, and three electroacupuncture treatments were given in each of the three electroacupuncture groups within 24 hours. The interval between two electroacupuncture treatments was 7 hours and the time of retention was 40min (Shu-point electroacupuncture group: bilateral Feshu and large intestine Yu for 40 minutes; The control group was divided into two groups: bilateral Zhongfu point and Tianshu point for 40 minutes, Shu group for 20 minutes and bilateral Zhongfu point and Tianshu point for 20mmin). Empty self-group and model group are given the same time binding. Samples were collected at 36 h after modeling. The supernatant was taken from the supernatant of cardiac blood. Serum amylase, (amylase, AMY), cholecystokinin (cholecystokinin, CCK), vasoactive intestinal peptide (vasoactive intestinal peptide, VIP), motilin (motilin, MTL) (vasoactive intestinal peptide, VIP),) and diamine oxidase (Diamine Oxidase,) were measured by ELISA. DAO); Inflammatory injury factors were measured in lung, pancreas and large intestine. Myeloperoxidase (myeloperxidase, MPO) and malondialdehyde (malondialdehyde, MDA),) were measured by ELISA. Results: after electroacupuncture treatment, MPO,MDA and AMY,VIP in lung, pancreas, large intestine and serum in three electroacupuncture groups were significantly lower than those in model group (P0.05), serum MTL was significantly increased (P0.05). The pathological score and serum CCK,DAO of the model group were significantly lower than that of the model group (P0.05). In serum VIP,MTL, lung and large intestine tissue MPO,MDA, there was statistical difference among the three electroacupuncture treatment groups. The Shu-Fu point electroacupuncture group was better than the simple Yu acupoint electroacupuncture group or the Jun acupoint electroacupuncture group. Conclusion: both the two channels of lung and large intestine can reduce lung injury by regulating gastrointestinal hormones, promoting gastrointestinal motility and improving gastrointestinal dysfunction.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245.97
本文編號：2367801
[Abstract]:Background: acute lung injury (acute lung injury, ALI) is a common complication of severe acute pancreatitis (severe acute pancreatitis, SAP). Previous studies have shown that acupuncture can alleviate lung inflammation by regulating gastrointestinal hormone levels and improving gastrointestinal dysfunction in SAP rats. However, the mechanism of electroacupuncture on (acute pancreatitis, AP) associated ALI and the optimal acupuncture of acupoints in the treatment of acute pancreatitis were not clear, but the two meridians of lung and large intestine were combined with acupoint electroacupuncture. Objective: based on the theory of "lung and large intestine" and taking the intestinal dysfunction and lung injury that occur most frequently in SAP as the starting point, the author chose the point of Shu-fu-pairing of lung and large intestine to study the mechanism of improving gastrointestinal function and preventing and treating ALl by electroacupuncture of Yu-fu-pin. Objective: to explore the principle of therapeutic effect and the best combination of acupoints in the treatment of SAP. Methods: forty male Sprague-Dawley (SD) rats were randomly divided into three groups: blank group, model group, electroacupuncture group at Shu point, electroacupuncture group at Jun acupoint and electroacupuncture group at Shufu point (8 rats in each group). The SAP model was made by injecting 3.5% sodium taurocholate retrograde pump into the pancreaticobiliary duct in both the electroacupuncture group and the model group. Two hours after the establishment of the model, electroacupuncture was given, and three electroacupuncture treatments were given in each of the three electroacupuncture groups within 24 hours. The interval between two electroacupuncture treatments was 7 hours and the time of retention was 40min (Shu-point electroacupuncture group: bilateral Feshu and large intestine Yu for 40 minutes; The control group was divided into two groups: bilateral Zhongfu point and Tianshu point for 40 minutes, Shu group for 20 minutes and bilateral Zhongfu point and Tianshu point for 20mmin). Empty self-group and model group are given the same time binding. Samples were collected at 36 h after modeling. The supernatant was taken from the supernatant of cardiac blood. Serum amylase, (amylase, AMY), cholecystokinin (cholecystokinin, CCK), vasoactive intestinal peptide (vasoactive intestinal peptide, VIP), motilin (motilin, MTL) (vasoactive intestinal peptide, VIP),) and diamine oxidase (Diamine Oxidase,) were measured by ELISA. DAO); Inflammatory injury factors were measured in lung, pancreas and large intestine. Myeloperoxidase (myeloperxidase, MPO) and malondialdehyde (malondialdehyde, MDA),) were measured by ELISA. Results: after electroacupuncture treatment, MPO,MDA and AMY,VIP in lung, pancreas, large intestine and serum in three electroacupuncture groups were significantly lower than those in model group (P0.05), serum MTL was significantly increased (P0.05). The pathological score and serum CCK,DAO of the model group were significantly lower than that of the model group (P0.05). In serum VIP,MTL, lung and large intestine tissue MPO,MDA, there was statistical difference among the three electroacupuncture treatment groups. The Shu-Fu point electroacupuncture group was better than the simple Yu acupoint electroacupuncture group or the Jun acupoint electroacupuncture group. Conclusion: both the two channels of lung and large intestine can reduce lung injury by regulating gastrointestinal hormones, promoting gastrointestinal motility and improving gastrointestinal dysfunction.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245.97
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