【摘要】：目的:觀察電針對局灶性腦缺血大鼠丘腦腹后外側(cè)核(VPL)神經(jīng)生長因子(NGF)和生長休止特定蛋白7(Gas 7)表達的影響,探討電針治療局灶性腦缺血繼發(fā)性損傷的可能機制。方法:采用隨機數(shù)字表法將48只SD大鼠分為正常組、假手術(shù)組、模型組和缺血后電針治療組(簡稱電針組),每組12只。采用線栓法復制右側(cè)大腦中動脈栓塞模型。電針組電針"百會"和左側(cè)"足三里"穴,每天1次,每次30 min,連續(xù)21d。免疫組織化學法和Western blot法檢測大鼠右側(cè)VPL中NGF和Gas 7的表達;尼氏染色法觀察VPL神經(jīng)元形態(tài)。結(jié)果:尼氏染色結(jié)果顯示,正常組及假手術(shù)組右側(cè)VPL結(jié)構(gòu)清晰完整,胞核居中,核仁清楚;模型組VPL神經(jīng)元染色較深,細胞核固縮;電針組神經(jīng)細胞形態(tài)與正常組接近。免疫組織化學和Western blot檢測結(jié)果一致,假手術(shù)組右側(cè)VPL中NGF和Gas 7蛋白的表達與正常組比較差異無統(tǒng)計學意義(P0.05);與正常組比較,模型組缺血側(cè)VPL中NGF和Gas 7蛋白表達明顯升高(P0.05);與模型組比較,電針組缺血側(cè)VPL中NGF和Gas 7蛋白表達顯著升高(P0.05)。結(jié)論:局灶性腦缺血大鼠缺血側(cè)VPL中NGF和Gas 7的表達增強,可能參與了機體代償性恢復的某些過程;電針可上調(diào)缺血側(cè)VPL中NGF和Gas 7的表達,可能是電針改善腦缺血繼發(fā)性丘腦損害的機制之一。
[Abstract]:Objective: to observe the effect of electroacupuncture on the expression of (VPL) nerve growth factor (NGF) and growth rest specific protein 7 (Gas 7) in ventrolateral nucleus of thalamus of rats with focal cerebral ischemia. To explore the possible mechanism of electroacupuncture in treating secondary injury of focal cerebral ischemia. Methods: 48 SD rats were randomly divided into normal group, sham operation group, model group and electroacupuncture group after ischemia (12 rats in each group). The right middle cerebral artery (MCA) embolization model was established by thread embolization. In the electroacupuncture group, the points of Baihui and Zusanli on the left side were treated once a day for 30 min, for 21 days. The expression of NGF and Gas 7 in the right VPL of rats was detected by immunohistochemistry and Western blot, and the morphology of VPL neurons was observed by Nissl staining. Results: the results of Nissl staining showed that the right VPL structure of normal group and sham operation group was clear and complete, the nucleus was in the middle, the nucleolus was clear, the VPL neurons in the model group were stained deeply and the nucleus was pyknosis, and the morphology of nerve cells in the electroacupuncture group was close to that in the normal group. The expression of NGF and Gas 7 protein in VPL of sham-operated group was not significantly different from that of normal group (P0.05). Compared with the normal group, the expression of NGF and Gas 7 protein in VPL of the model group was significantly higher than that in the model group (P0.05); compared with the model group, the expression of NGF and Gas 7 protein in the VPL of the electroacupuncture group was significantly higher (P0.05). Conclusion: the increased expression of NGF and Gas 7 in VPL of rats with focal cerebral ischemia may be involved in the process of compensatory recovery. Electroacupuncture can up-regulate the expression of NGF and Gas 7 in VPL of ischemic side, which may be one of the mechanisms of electroacupuncture in ameliorating the secondary thalamic damage after cerebral ischemia.
【作者單位】： 皖南醫(yī)學院人體解剖學教研室;
【基金】：皖南醫(yī)學院中青年科研基金項目(No.WK 201509);皖南醫(yī)學院校重點科研項目培育基金項目(No.WK 2016Z09) 安徽省青年自然科學基金(No.1608085QH 223) 安徽省名師工作室(No.2014msgzs 152)
【分類號】：R245.97

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