白芥子涂法穴位貼敷對豚鼠支氣管哮喘氣道高反應(yīng)性和肺組織TRPV1表達(dá)影響的研究
發(fā)布時(shí)間：2018-11-19 18:34
【摘要】：目的:研究白芥子涂法穴位貼敷對哮喘豚鼠的抗氣道高反應(yīng)性作用,并初步探討其機(jī)制。方法:將豚鼠分為正常組、哮喘組、貼敷治療組。模型采用OVA致敏法,首次激發(fā)后,行每周一次的白芥子涂法貼敷,共3次。末次激發(fā)后24 h,小動(dòng)物呼吸功能測試儀檢測呼氣阻力、吸氣阻力、肺順應(yīng)性,HE染色檢測肺組織炎癥,免疫組化法檢測肺組織TRPV1的定位與表達(dá),免疫印跡法檢測肺組織TRPV1蛋白的表達(dá)水平。結(jié)果:與正常組相比,哮喘組豚鼠呼氣阻力、吸氣阻力明顯上升,肺順應(yīng)性明顯下降,TRPV1表達(dá)略有上升(P0.05);與哮喘組相比,貼敷治療組豚鼠哮喘組豚鼠呼氣阻力、吸氣阻力顯著下降,肺順應(yīng)性上升,TRPV1表達(dá)顯著下調(diào)(P0.05)。結(jié)論:白芥子涂法穴位貼敷可能通過調(diào)節(jié)肺組織TRPV1,降低哮喘中的氣道高反應(yīng)性感性。
[Abstract]:Aim: to study the anti-airway hyperresponsiveness of white mustard-coated acupoint on asthmatic guinea pigs and to explore its mechanism. Methods: the guinea pigs were divided into normal group, asthma group and application treatment group. The model was sensitized by OVA. After the first excitation, the white mustard coating was applied once a week for 3 times. Breath resistance, inspiratory resistance, lung compliance, HE staining and lung inflammation were detected 24 hours after the last challenge. The localization and expression of TRPV1 in lung tissue were detected by immunohistochemical method. The expression of TRPV1 protein was detected by Western blot. Results: compared with the normal group, the expiratory resistance, inspiratory resistance and lung compliance of asthmatic guinea pigs increased significantly, and the expression of TRPV1 increased slightly (P0.05). Compared with the asthma group, the airway resistance, inspiratory resistance, lung compliance and TRPV1 expression were significantly decreased in the treatment group (P0.05). Conclusion: acupoint application with white mustard coating may reduce airway hyperresponsiveness by regulating TRPV1, in lung tissue.
【作者單位】： 遼寧中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金青年項(xiàng)目(81603528) 沈陽市科技局重點(diǎn)實(shí)驗(yàn)室項(xiàng)目(F13-285-1-00)
【分類號】：R245
本文編號：2343082
[Abstract]:Aim: to study the anti-airway hyperresponsiveness of white mustard-coated acupoint on asthmatic guinea pigs and to explore its mechanism. Methods: the guinea pigs were divided into normal group, asthma group and application treatment group. The model was sensitized by OVA. After the first excitation, the white mustard coating was applied once a week for 3 times. Breath resistance, inspiratory resistance, lung compliance, HE staining and lung inflammation were detected 24 hours after the last challenge. The localization and expression of TRPV1 in lung tissue were detected by immunohistochemical method. The expression of TRPV1 protein was detected by Western blot. Results: compared with the normal group, the expiratory resistance, inspiratory resistance and lung compliance of asthmatic guinea pigs increased significantly, and the expression of TRPV1 increased slightly (P0.05). Compared with the asthma group, the airway resistance, inspiratory resistance, lung compliance and TRPV1 expression were significantly decreased in the treatment group (P0.05). Conclusion: acupoint application with white mustard coating may reduce airway hyperresponsiveness by regulating TRPV1, in lung tissue.
【作者單位】： 遼寧中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金青年項(xiàng)目(81603528) 沈陽市科技局重點(diǎn)實(shí)驗(yàn)室項(xiàng)目(F13-285-1-00)
【分類號】：R245
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