發(fā)布時(shí)間：2018-11-18 07:54

【摘要】：目的:通過(guò)背俞指針療法治療胃食管反流病大鼠,觀察SCF-c-kit-ICC信號(hào)通路的基因、蛋白表達(dá)情況和ICC細(xì)胞突起變化,探討背俞指針療法對(duì)上述變化的影響,以期揭示背俞指針療法治療GERD作用機(jī)理。方法:將60只GERD大鼠采用隨機(jī)數(shù)字表法,將60只SD大鼠隨機(jī)平均分為4組,每組15只,空白組和模型組不予干預(yù),參照采用賁門(mén)鋼圈固定法進(jìn)行造模,指針治療組予背俞指針療法干預(yù)治療,西藥對(duì)照組予枸櫞酸莫沙必利分散片聯(lián)合蘭索拉唑腸溶片灌胃,兩組治療療程均為2周。干預(yù)周期結(jié)束后取4組大鼠胃起搏區(qū)平滑肌組織若干塊,約重100-150mg,活檢標(biāo)本立即投入液氮中,1天后轉(zhuǎn)入-80℃冰箱中保存,另有部分標(biāo)本置入2.5%戊二醛固定液中保存。本次試驗(yàn)進(jìn)行檢測(cè)指標(biāo)如下:(1)Real-timePCR法觀察SCF/c-kit信號(hào)通路中SCFmRNA、c-kitmRNA的表達(dá)情況;(2)Westernblot法檢測(cè)GERD模型大鼠胃起搏區(qū)平滑肌組織中SCF、c-kit的蛋白質(zhì)表達(dá)情況;(3)透射電鏡觀察GERD模型大鼠胃起搏區(qū)平滑肌組織中Cajal細(xì)胞數(shù)目及其超微結(jié)構(gòu)的影響。采用SPSS22.0統(tǒng)計(jì)軟件,通過(guò)t檢驗(yàn)或X2檢驗(yàn)對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行統(tǒng)計(jì)分析。若P0.05時(shí)則認(rèn)為檢驗(yàn)結(jié)果差異具有統(tǒng)計(jì)學(xué)意義。結(jié)果:1、造模前,各組大鼠體重比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。2、模型組的SCFmRNA和c-kitRNA在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的表達(dá)較空白組下調(diào)(P0.05),具有統(tǒng)計(jì)學(xué)意義;指針治療組和西藥對(duì)照組的SCFmRNA和c-kitmRNA在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的表達(dá)較模型組顯著升高(P0.01),具有統(tǒng)計(jì)學(xué)意義;指針治療組和西藥對(duì)照組的SCFmRNA和c-kitmRNA在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的表達(dá)相比無(wú)明顯差異(P0.05),無(wú)統(tǒng)計(jì)學(xué)意義,并與正常組相比無(wú)顯著性差異(P0.05),無(wú)統(tǒng)計(jì)學(xué)意義。3、GERD大鼠模型組的SCF和c-kit在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的蛋白表達(dá)較空白組下調(diào)(P0.05),具有統(tǒng)計(jì)學(xué)意義;指針治療組和西藥對(duì)照組的SCF和c-kit在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的蛋白表達(dá)較模型組顯著升高(P0.01),具有統(tǒng)計(jì)學(xué)意義;指針治療組和西藥對(duì)照組的SCF和c-kit在胃運(yùn)動(dòng)起搏區(qū)平滑肌組織的蛋白表達(dá)相比無(wú)明顯差異(P0.05),無(wú)統(tǒng)計(jì)學(xué)意義,并與正常組相比無(wú)顯著性差異(P0.05),無(wú)統(tǒng)計(jì)學(xué)意義。4、模型組ICC細(xì)胞數(shù)量和超微結(jié)構(gòu)明顯改變,突起數(shù)量明顯減少,突起長(zhǎng)度減短,與平滑肌細(xì)胞之間的縫隙連接明顯減少,且線(xiàn)粒體大多呈空泡樣改變。指針治療組、西藥對(duì)照組和模型組相比,ICC細(xì)胞數(shù)量和超微結(jié)構(gòu)有明顯改善,以及與平滑肌細(xì)胞之間的細(xì)胞突起數(shù)量及長(zhǎng)度、縫隙連接有明顯改善。結(jié)論:背俞指針療法可能通過(guò)調(diào)控SCF-c-kit-ICC信號(hào)通路的mRNA基因水平的表達(dá)、蛋白水平的表達(dá)及細(xì)胞形態(tài)的改變,促進(jìn)GERD的胃腸動(dòng)力恢復(fù)。
[Abstract]:Objective: to observe the gene and protein expression of SCF-c-kit-ICC signal pathway and the changes of ICC cell processes in rats with gastroesophageal reflux disease treated with back Shu pointer therapy. The purpose of this study was to reveal the mechanism of the treatment of GERD with back-Shu pointer therapy. Methods: sixty GERD rats were randomly divided into 4 groups (15 rats in each group) by random digital table method. The blank group and model group were not interfered, and the model was made with the method of cardia ring fixation. The control group was treated with mosapride citrate dispersible tablets combined with lansoprazole enteric-coated tablets for 2 weeks. At the end of the intervention cycle, a number of smooth muscle tissues of gastric pacing area were taken from four groups of rats, weighing about 100-150 mg. The biopsy specimens were immediately injected into liquid nitrogen, and then transferred to -80 鈩，

本文編號(hào)：2339372