小艾炷直接灸“肝俞”穴對HCC大鼠成模期血清肝功能及AFP、TNF-α的影響
發(fā)布時(shí)間：2018-11-16 09:43
【摘要】：原發(fā)性肝癌(HepatocellularCarcinoma,HCC)是全球高發(fā)病率和高死亡率的惡性腫瘤之一。相關(guān)文獻(xiàn)研究表明,灸法防治肝癌有一定的臨床療效基礎(chǔ),并且灸法防治疾病已有幾千年的歷史。因此,本研究采用小艾炷逆灸大鼠的"肝俞"穴,觀察直接灸和隔姜灸對大鼠肝功能及腫瘤相關(guān)因子的影響,探討逆灸法對原發(fā)性肝癌的預(yù)防作用。目的本實(shí)驗(yàn)以DEN誘導(dǎo)的HCC模型成模期大鼠為研究對象,以"肝俞"穴為施灸部位,造模的同時(shí)對大鼠進(jìn)行直接灸和隔姜灸干預(yù)。觀察直接灸和隔姜灸法對HCC模型成模期大鼠肝臟病理組織形態(tài)學(xué),以及肝功能天冬氨酸氨基轉(zhuǎn)移酶(Aspartate transaminase,AST),γ-谷氨酰轉(zhuǎn)肽酶(Glutamyl transpeptidase,GGT)、堿性磷酸酶(Alkaline phosphatas,ALP)、甲胎蛋白(Alpha-fetoproteinheterop-lasmon,AFP)和腫瘤壞死因子(Tumor necrosis factor-α,TNF-α)的影響,以探討該灸法對HCC模型大鼠成模期的預(yù)防作用,并評價(jià)兩種灸量以及不同灸法對HCC模型大鼠的預(yù)防效應(yīng)。方法將70只清潔級wistar雄性大鼠隨機(jī)分為空白組(10只)、模型組(15只)、直接灸1組(15只)、直接灸2組(15只)、隔姜灸組(15只)。除空白組外,各組大鼠按照50mglkg的劑量,腹腔注射濃度為l0mg/ml的DEN溶液,每周2次,每次間隔3或4d(約84h),連續(xù)10周;直接灸1組灸"肝俞"穴,3壯/穴;直接灸2組灸"肝俞"穴,6壯/穴,均隔日1次,連續(xù)10周,每壯艾炷灸完再換;隔姜灸組需在"肝俞"穴上放置一 2mm厚的姜片,灸"肝俞"穴,3壯/穴,每壯艾炷灸完再換;造模結(jié)束后,對大鼠肝臟病理形態(tài)學(xué)變化用HE染色法觀察;用全自動(dòng)生化分析儀對各組大鼠血清AST、GGT、ALP進(jìn)行檢測,用ELISA法對各組大鼠血清中AFP、TNF-α進(jìn)行檢測。結(jié)果1肝組織病理形態(tài)學(xué)變化:空白組大鼠肝細(xì)胞胞漿胞核均勻、肝細(xì)胞索排列整齊,呈放射狀,肝細(xì)胞形態(tài)正常;模型組腫瘤細(xì)胞成團(tuán)塊狀分布,小葉結(jié)構(gòu)完全破壞,核/質(zhì)比增大,細(xì)胞核深染,異型性增加,肝細(xì)胞壞死,部分出現(xiàn)核固縮和核碎裂現(xiàn)象;直接灸1組,核/質(zhì)比略增大,肝癌細(xì)胞數(shù)量較少,細(xì)胞核異型性低,有少量結(jié)節(jié)形成;直接灸2組,細(xì)胞核深染、核/質(zhì)比增大,肝細(xì)胞有雙核;隔姜灸組,細(xì)胞核深染、核/質(zhì)比略增大,有肝細(xì)胞索和肝血竇結(jié)構(gòu)。2血清肝功能表達(dá)情況:與空白組相比,模型組大鼠血清AST、GGT、ALP含量均顯著升高(P0.05);與模型組相比,直接灸1組、直接灸2組大鼠血清GGT含量均顯著降低(P0.05),隔姜灸組大鼠血清AST、GGT、ALP含量均顯著降低(P0.05)。3血清AFP表達(dá)情況:與空白組相比,模型組大鼠血清AFP含量顯著增高(P0.05);與模型組比較,直接灸1組、隔姜灸組大鼠的血清AFP含量均顯著降低(P0.05)。4血清TNF-α表達(dá)情況:與空白組比較,模型組大鼠血清TNF-α含量顯著增高(P0.05);與模型組比較,直接灸1組大鼠血清TNF-α含量顯著降低(P0.05)。結(jié)論1小艾炷直接灸和隔姜灸均能改善HCC大鼠成模期的肝臟病理形態(tài),減緩細(xì)胞癌變進(jìn)程。2小艾炷直接灸和隔姜灸均能有效降低HCC大鼠成模期血清GGT含量。3直接灸1組能有效降低HCC大鼠成模期血清AFP和TNF-α含量,說明直接灸1組可以通過減緩DEN對大鼠肝細(xì)胞的炎性反應(yīng),抑制肝癌細(xì)胞的增長。4小艾炷隔姜灸能有效降低HCC大鼠成模期血清AST、GGT、ALP及AFP含量,說明隔姜灸組能夠明顯改善大鼠的肝功能,減輕肝細(xì)胞的損傷,抑制肝癌細(xì)胞的增長。5小艾炷直接灸和隔姜灸在一定程度上對HCC模型大鼠具有預(yù)防作用,可以減緩肝臟癌變進(jìn)程,其內(nèi)在機(jī)制可能是通過減輕DEN對大鼠肝細(xì)胞的炎性反應(yīng)、改善肝細(xì)胞膜通透性、調(diào)節(jié)TNF-α介導(dǎo)的信號通路等幾方面去降低肝細(xì)胞的癌變程度。
[Abstract]:Primary liver cancer (HCC) is one of the most high-and high-mortality malignant tumors in the world. The relevant literature studies have shown that the moxibustion method has a certain clinical curative effect on the prevention and treatment of liver cancer, and the moxibustion method has a history of several thousand years. Therefore, the effect of direct moxibustion and ginger moxibustion on the liver function and tumor-related factors in rats was observed by using the "liver Yu" point of the small-Ai-type counter-moxibustion rats, and the prevention effect of the reverse-moxibustion method on the primary liver cancer was discussed. Objective To study the effect of direct moxibustion and moxibustion on the rats by using DEN-induced HCC model as the study object and using the "liver Yu" point as the moxibustion part and making the model. The morphology of the liver and the morphology of liver and the liver function aspartate aminotransferase (GGT), alkaline phosphatase (ALP) and alpha-fetoroteinhaetop-lamon were observed in the model of HCC. The effect of the moxibustion on the model period of HCC model rats was discussed, and the preventive effects of different moxibustion methods on the model rats of HCC were evaluated. Methods 70 healthy male rats were randomly divided into blank group (n = 10), model group (15), direct-moxibustion group 1 (15), direct-moxibustion group 2 (15), and ginger-type moxibustion group (15 only). In addition to the blank group, the rats in each group were given a dose of 50mglkg, a DEN solution with a concentration of l0mg/ ml in the abdominal cavity, 2 times a week, 3 or 4 days at a time (about 84h), for 10 weeks for a continuous period of time, 1 group of moxibustion "liver Yu" of the direct-moxibustion group and 3 strong/ a hole, and the direct-moxibustion 2-group moxibustion "liver Yu" point, the 6-strong/ acupoint and the soaking time are once, In order to change the pathological changes of the liver of the rats, the pathological changes of the liver of the rats were observed with HE staining. The serum AST, GGT and ALP of each group were detected by a full-automatic biochemical analyzer, and AFP and TNF-1 in the serum of each group were detected by ELISA. Results: The changes of the pathological morphology of the liver tissue were as follows: the cytoplasm of the hepatocytes in the blank group was homogeneous, the liver cells were arranged in order, and the liver cells were normal; the tumor cells of the model group were clustered and distributed, the leaflet structure was completely destroyed, the nuclear/ mass ratio was increased, and the cell nucleus was deeply stained. in that direct-moxibustion group 1, the nuclear-to-mass ratio is slightly increase, the number of the liver cancer cells is small, the nuclear atypia is low, and a small number of nodules are formed; the direct-moxibustion group 2, the nuclear deep dye and the nuclear/ mass ratio increase, The serum AST, GGT and ALP in the model group were significantly higher than that of the blank group (P0.05). The levels of serum GGT in the rats of the direct-moxibustion group and the direct-moxibustion group were significantly lower (P0.05). The serum levels of AST, GGT and ALP in the rats were significantly lower than that of the blank group (P0.05). The serum AFP level in the model group was significantly higher than that of the blank group (P0.05); and compared with the model group, The levels of serum AFP in the rats were significantly lower than those in the blank group (P0.05). The serum TNF-1 content in the model group was significantly higher in the model group than in the blank group (P0.05); and compared with the model group, The serum TNF-1 content in the 1 group of the direct-moxibustion group was significantly lower (P0.05). Conclusion: 1. The direct moxibustion and the moxibustion with ginger can improve the pathological morphology of the liver in the model of HCC. in ord to slow down that process of cell canceration, the serum GGT content in the rat model of HCC can be effectively reduced by the direct moxibustion and the direct moxibustion, and the direct moxibustion 1 group can effectively reduce the content of serum AFP and TNF-1 in the model phase of the rat with HCC, and the direct moxibustion group 1 can reduce the inflammatory reaction of DEN to the rat liver cells. The increase of serum AST, GGT, ALP and AFP in the rat model of HCC can be effectively reduced, and the effect of the ginger moxibustion group on the liver function of the rat can be obviously improved, and the damage of the liver cells can be reduced. It is possible to reduce the inflammatory response of DEN to the rat's liver cells and to improve the permeability of the liver cell membrane. TNF-mediated signaling pathway and the like are regulated to reduce the degree of carcinogenesis of the liver cells.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245
本文編號：2335168
[Abstract]:Primary liver cancer (HCC) is one of the most high-and high-mortality malignant tumors in the world. The relevant literature studies have shown that the moxibustion method has a certain clinical curative effect on the prevention and treatment of liver cancer, and the moxibustion method has a history of several thousand years. Therefore, the effect of direct moxibustion and ginger moxibustion on the liver function and tumor-related factors in rats was observed by using the "liver Yu" point of the small-Ai-type counter-moxibustion rats, and the prevention effect of the reverse-moxibustion method on the primary liver cancer was discussed. Objective To study the effect of direct moxibustion and moxibustion on the rats by using DEN-induced HCC model as the study object and using the "liver Yu" point as the moxibustion part and making the model. The morphology of the liver and the morphology of liver and the liver function aspartate aminotransferase (GGT), alkaline phosphatase (ALP) and alpha-fetoroteinhaetop-lamon were observed in the model of HCC. The effect of the moxibustion on the model period of HCC model rats was discussed, and the preventive effects of different moxibustion methods on the model rats of HCC were evaluated. Methods 70 healthy male rats were randomly divided into blank group (n = 10), model group (15), direct-moxibustion group 1 (15), direct-moxibustion group 2 (15), and ginger-type moxibustion group (15 only). In addition to the blank group, the rats in each group were given a dose of 50mglkg, a DEN solution with a concentration of l0mg/ ml in the abdominal cavity, 2 times a week, 3 or 4 days at a time (about 84h), for 10 weeks for a continuous period of time, 1 group of moxibustion "liver Yu" of the direct-moxibustion group and 3 strong/ a hole, and the direct-moxibustion 2-group moxibustion "liver Yu" point, the 6-strong/ acupoint and the soaking time are once, In order to change the pathological changes of the liver of the rats, the pathological changes of the liver of the rats were observed with HE staining. The serum AST, GGT and ALP of each group were detected by a full-automatic biochemical analyzer, and AFP and TNF-1 in the serum of each group were detected by ELISA. Results: The changes of the pathological morphology of the liver tissue were as follows: the cytoplasm of the hepatocytes in the blank group was homogeneous, the liver cells were arranged in order, and the liver cells were normal; the tumor cells of the model group were clustered and distributed, the leaflet structure was completely destroyed, the nuclear/ mass ratio was increased, and the cell nucleus was deeply stained. in that direct-moxibustion group 1, the nuclear-to-mass ratio is slightly increase, the number of the liver cancer cells is small, the nuclear atypia is low, and a small number of nodules are formed; the direct-moxibustion group 2, the nuclear deep dye and the nuclear/ mass ratio increase, The serum AST, GGT and ALP in the model group were significantly higher than that of the blank group (P0.05). The levels of serum GGT in the rats of the direct-moxibustion group and the direct-moxibustion group were significantly lower (P0.05). The serum levels of AST, GGT and ALP in the rats were significantly lower than that of the blank group (P0.05). The serum AFP level in the model group was significantly higher than that of the blank group (P0.05); and compared with the model group, The levels of serum AFP in the rats were significantly lower than those in the blank group (P0.05). The serum TNF-1 content in the model group was significantly higher in the model group than in the blank group (P0.05); and compared with the model group, The serum TNF-1 content in the 1 group of the direct-moxibustion group was significantly lower (P0.05). Conclusion: 1. The direct moxibustion and the moxibustion with ginger can improve the pathological morphology of the liver in the model of HCC. in ord to slow down that process of cell canceration, the serum GGT content in the rat model of HCC can be effectively reduced by the direct moxibustion and the direct moxibustion, and the direct moxibustion 1 group can effectively reduce the content of serum AFP and TNF-1 in the model phase of the rat with HCC, and the direct moxibustion group 1 can reduce the inflammatory reaction of DEN to the rat liver cells. The increase of serum AST, GGT, ALP and AFP in the rat model of HCC can be effectively reduced, and the effect of the ginger moxibustion group on the liver function of the rat can be obviously improved, and the damage of the liver cells can be reduced. It is possible to reduce the inflammatory response of DEN to the rat's liver cells and to improve the permeability of the liver cell membrane. TNF-mediated signaling pathway and the like are regulated to reduce the degree of carcinogenesis of the liver cells.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 諸佳瑜;徐望;陳闖;歐杰;毛素菲;莫曉麗;陸冬玲;;隔姜灸聯(lián)合經(jīng)肝動(dòng)脈化療栓塞術(shù)治療肝郁脾虛型原發(fā)性肝癌的效果觀察[J];臨床肝膽病雜志;2017年01期
2 章健;來維潔;周秀梅;王毅剛;;靶向肝癌干細(xì)胞的腫瘤治療進(jìn)展[J];中國細(xì)胞生物學(xué)學(xué)報(bào);2017年01期
3 朱歌;趙玲玲;錢磊;董亞冰;趙恒軍;崔久嵬;王楠婭;;血清ALP、GGT水平對射頻消融術(shù)治療原發(fā)性肝癌患者預(yù)后的影響[J];臨床肝膽病雜志;2016年12期
4 張少鵬;姚樹坤;宋威江;李蕊;;中藥單體治療原發(fā)性肝癌實(shí)驗(yàn)研究進(jìn)展[J];中日友好醫(yī)院學(xué)報(bào);2016年05期
5 朱吉穎;周紅蔚;;隔姜灸足三里穴對緩解腫瘤患者癌因性疲乏的效果觀察[J];上海護(hù)理;2016年05期
6 李玲;高翠霞;何煒;馬桂霞;王煒煒;司慧彬;孟雨姍;;溫陽艾灸法聯(lián)合三階梯止痛法對癌痛患者止痛效果和生活質(zhì)量的影響[J];中醫(yī)研究;2016年09期
7 閆亞南;王寧;王卓昱;田瑤;成宇鎮(zhèn);馬惠芳;侯中偉;馬佳佳;關(guān)翰宇;;直接灸“肝俞”對原發(fā)性肝癌癌前病變大鼠T淋巴細(xì)胞亞群的影響[J];針刺研究;2016年04期
8 斯韜;寧雪堅(jiān);馮獻(xiàn)斌;石_g;馮馭臣;李蓉;;肝癌放療期間中醫(yī)證候演變規(guī)律[J];河南中醫(yī);2016年07期
9 袁征;陳曉婷;;原發(fā)性肝癌超聲表現(xiàn)與中醫(yī)分型的關(guān)系[J];中華中醫(yī)藥學(xué)刊;2016年06期
10 閃海霞;朱幼芙;范崇桂;張懷宏;;Wnt信號通路對肝細(xì)胞肝癌的影響[J];廣東醫(yī)學(xué);2016年04期
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