【摘要】：目的:通過蒙藥肋柱花不同溶劑提取物及活性部位配伍藥理作用研究,篩選肋柱花抗炎、鎮(zhèn)痛、抗急性肝損傷有效活性部位。方法:將小鼠按體重分成若干組,通過二甲苯耳腫脹抗炎實驗,計算腫脹度和腫脹抑制率(%);通過醋酸扭體實驗,記錄小鼠開始扭體時間和內(nèi)扭體次數(shù);通過抗D-氨基半乳糖胺急性肝損傷藥理實驗,檢測小鼠血清ALT、AST,觀察肝組織病理變化。結(jié)果:1.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的降低小鼠耳腫脹度,p0.05;肋柱花石油醚、二氯甲烷、乙酸乙酯、乙醇提取部位具有一定的抑制小鼠耳腫脹作用。2.肋柱花石油醚、乙醇提取物具有一定的延長小鼠開始扭體時間和減少扭體次數(shù),p0.05。3.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的降低血清AST作用,p0.05;肋柱花乙酸乙酯、乙醇提取物具有一定的降低血清ALT作用,p0.05。4.肋柱花石油醚:乙醇提取物配伍組(1:9);二氯甲烷:乙酸乙酯:乙醇提取物配伍組(1:2:9)具有一定的降低小鼠血清AST、ALT作用,p0.05或p0.01。5.肋柱花石油醚:乙醇(1:9)配伍組大、中、小劑量組具有一定的降低小鼠耳腫脹度和抑制小鼠耳腫脹作用;肋柱花石油醚:乙醇(1:9)配伍組大、中劑量組具有一定的延長小鼠開始扭體時間和減少扭體次數(shù),p0.05;肋柱花石油醚:乙醇(1:9)配伍組大、中劑量組具有一定的降低血清AST、ALT作用,p0.05。結(jié)論:1.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的抗炎活性。2.肋柱花石油醚、乙醇提取物具有一定的鎮(zhèn)痛作用。3.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的保肝活性。4.肋柱花石油醚:乙醇提取物配伍組(1:9);二氯甲烷:乙酸乙酯:乙醇提取物配伍組(1:2:9)具有顯著的保肝活性。5.肋柱花石油醚:乙醇(1:9)配伍組大、中、小劑量組具有一定的抗炎、鎮(zhèn)痛、保肝活性。6.通過以上實驗篩選出蒙藥肋柱花抗炎、鎮(zhèn)痛、保肝有效活性部位,為進一步提取肋柱花保肝活性成分提供科學(xué)依據(jù)。
[Abstract]:Objective: to study the pharmacological effects of different solvent extracts and active fractions of Lumbellae mongolicum on anti-inflammatory, analgesic and anti-acute liver injury. Methods: mice were divided into several groups according to their body weight. The swelling degree and swelling inhibition rate (%) were calculated by xylene ear swelling and anti-inflammatory test, and the time of writhing and the times of internal writhing were recorded by acetic acid writhing test. The pathological changes of liver tissue were detected by ALT,AST, in serum of mice by pharmacological experiment of anti-D-galactosamine acute liver injury. The result is 1: 1. The extract of petroleum ether, ethyl acetate and ethanol could reduce the ear swelling in mice (p0.05), and the extracts of petroleum ether, dichloromethane, ethyl acetate and ethanol could inhibit the ear swelling in mice. 2. The petroleum ether and ethanol extract of Flos costolata could prolong the time of writhing and reduce the times of writhing in mice (p0.05.3). The extracts of petroleum ether, ethyl acetate and ethanol had a certain effect on reducing serum AST (p0.05), while ethyl acetate and ethanol extracts from costal flower had a certain effect on reducing serum ALT (p0.05.4). The mixture group of petroleum ether and ethanol extract (1:9) and dichloromethane: ethyl acetate: ethanol extract (1:2:9) had the effect of decreasing serum AST,ALT in mice (p0.05 or p0.01.5). The large, medium and low dose groups of petroleum ether: ethanol (1:9) could reduce the degree of ear swelling in mice and inhibit the swelling of ears in mice to a certain extent. The combination of ethanol (1:9) and petroleum ether (1:9) was large, and the middle dose group could prolong the time of writhing and reduce the times of writhing in mice (p0.05). The effect of petroleum ether: ethanol (1:9) on serum AST,ALT was higher in the middle dose group than that in the middle dose group (p0.05). Conclusion: 1. The extracts of petroleum ether, ethyl acetate and ethanol have certain anti-inflammatory activity. 2. The extract of petroleum ether and ethanol has a certain analgesic effect. 3. The extracts of petroleum ether, ethyl acetate and ethanol have certain hepatoprotective activity. 4. The compatibility group of petroleum ether and ethanol extract (1:9) and dichloromethane: ethyl acetate: ethanol extract (1:2:9) had remarkable hepatoprotective activity. The large, medium and low dose groups of petroleum ether: ethanol (1:9) had some anti-inflammatory, analgesic and hepatoprotective activities. Through the above experiments, the effective active parts of the Mongolian medicine costal flower resistance, analgesia and liver preservation were selected, which provided scientific basis for the further extraction of liver-preserving active components from the costal style.
【學(xué)位授予單位】：內(nèi)蒙古民族大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29

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1 紅艷;;蒙藥肋柱花組織培養(yǎng)初探[J];北京農(nóng)業(yè);2016年03期

2 朱虹;張春紅;韋坤華;張樂;李e，

本文編號：2309750