【摘要】：目的:探討電針“內(nèi)關(guān)”與“太淵”穴預(yù)處理對急性心肌缺血再灌注損傷大鼠心肌的保護效應(yīng)差異及部分作用機制。方法:96只Wistar大鼠隨機分為假手術(shù)組、模型組、內(nèi)關(guān)組、太淵組,每組24只。內(nèi)關(guān)組與太淵組大鼠分別選取“內(nèi)關(guān)”與“太淵”穴進行電針預(yù)處理,刺激電流1mA,頻率2Hz,每次20min,每日1次,共干預(yù)7d。假手術(shù)組、模型組固定相同時間,不予電針干預(yù)。模型組、內(nèi)關(guān)組、太淵組大鼠在末次電針24h后進行心肌缺血再灌注模型復(fù)制,假手術(shù)組在開胸后僅在相應(yīng)部位用針空穿1次,不進行結(jié)扎。分別檢測各組大鼠心肌缺血面積、梗死面積,心肌組織蛋白激酶C(protein kinase C,PKC)活性及水通道蛋白1(aquaporin 1,AQP1)表達情況。結(jié)果:模型組大鼠的心肌缺血面積、梗死面積、AQP1蛋白表達及PKC的活性顯著升高,與假手術(shù)組比較差異均有統(tǒng)計學(xué)意義(均P0.01),內(nèi)關(guān)組大鼠的心肌缺血面積、梗死面積、PKC的活性和AQP1蛋白表達較模型組和太淵組均顯著減少(P0.01,P0.05)。經(jīng)Pearson相關(guān)分析,電針預(yù)處理后急性心肌缺血再灌注損傷大鼠心肌組織AQP1蛋白表達和PKC活性的變化呈顯著正相關(guān)。結(jié)論:電針“內(nèi)關(guān)”穴預(yù)處理可顯著降低急性心肌缺血再灌注損傷大鼠心肌組織AQP1蛋白表達和PKC活性,其作用機制可能是通過抑制PKC活化進而抑制AQP1蛋白表達,從而發(fā)揮其心肌保護效應(yīng)。
[Abstract]:Objective: to investigate the protective effects of electroacupuncture at "Neiguan" and "Taiyuan" points on acute myocardial ischemia-reperfusion injury in rats and its mechanism. Methods: 96 Wistar rats were randomly divided into sham-operation group, model group, Neiguan group and Taiyuan group, with 24 rats in each group. Neiguan group and Taiyuan group were treated by electroacupuncture pretreatment with "Neiguan" and "Taiyuan" acupoints respectively. The stimulation current was 1 Ma at the frequency of 2 Hz, 20 min each time, once a day, for 7 days. Sham operation group, model group fixed the same time, no electroacupuncture intervention. The model group, Neiguan group and Taiyuan group were treated with myocardial ischemia-reperfusion model 24 hours after the last electroacupuncture. Myocardial ischemia area, infarct area, myocardial protein kinase (C (protein kinase) activity and aquaporin-1 (aquaporin 1) expression were measured in each group. Results: the myocardial ischemia area, infarct area, AQP1 protein expression and PKC activity in the model group were significantly higher than those in the sham operation group (P0.01), and the myocardial ischemia area in the Neiguan group was significantly higher than that in the sham operation group (P0.01). The infarct area, the activity of PKC and the expression of AQP1 protein were significantly decreased compared with the model group and Taiyuan group (P 0.01 P 0.05). By Pearson correlation analysis, there was a significant positive correlation between the expression of AQP1 protein and the activity of PKC in myocardium of rats with acute myocardial ischemia-reperfusion injury after electroacupuncture preconditioning. Conclusion: electroacupuncture "Neiguan" point pretreatment can significantly reduce the expression of AQP1 protein and the activity of PKC in myocardial tissue of rats with acute myocardial ischemia-reperfusion injury, and its mechanism may be to inhibit the expression of AQP1 protein by inhibiting the activation of PKC. In order to play its myocardial protection effect.
【作者單位】： 安徽中醫(yī)藥大學(xué)針灸經(jīng)絡(luò)研究所;
【分類號】：R246.1

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