【摘要】：目的:研究電針刺激對脊髓損傷大鼠受損脊髓節(jié)段HIF-1a及VEGF表達(dá)的影響。方法:采用脊髓鉗夾法復(fù)制雌性SD大鼠脊髓損傷模型,在造模成功第3天后,隨機(jī)將大鼠分為夾脊組、胃經(jīng)組和損傷對照組,分別運(yùn)用電針刺激夾脊穴位、足陽明胃經(jīng)穴位治療以及不使用電針治療。期間使用BBB評分法定期對各組大鼠神經(jīng)功能評分進(jìn)行評價(jià),在治療14天后處死大鼠,取各組大鼠受損脊髓節(jié)段脊髓,行HE染色觀察組織受損情況;行免疫組織化學(xué)染色對比各組大鼠HIF-1a及VEGF蛋白表達(dá)情況;行qRT-PCR對比各組大鼠HIF-1a及VEGF的mRNA表達(dá)情況。結(jié)果:各組大鼠的BBB功能評分在治療前,治療1周均無明顯顯著差異,治療2周時(shí)BBB評分:陽明組夾脊組損傷對照組;HE染色顯示各組大鼠脊髓損傷情況符合其功能評分的表現(xiàn);免疫組織化學(xué)染色結(jié)果顯示,HIF-1a的表達(dá)在電針干預(yù)第7天:對照組陽明經(jīng)組夾脊穴組,電針干預(yù)第14天:陽明經(jīng)組夾脊穴組對照組,陽明經(jīng)組表達(dá)量:第7天第14天,夾脊穴組表達(dá)量:第7天第14天,對照組表達(dá)量:第7天第14天,VEGF的表達(dá)情況在第7天:對照組陽明經(jīng)組夾脊穴組,第14天:陽明經(jīng)組夾脊穴組對照組,陽明經(jīng)組表達(dá)量:第7天第14天,夾脊穴組表達(dá)量:第7天第14天,對照組表達(dá)量:第7天第14天;qRT-PCR結(jié)果顯示,HIF-1a的表達(dá)在電針干預(yù)第7天:夾脊穴組對照組陽明經(jīng)組,電針干預(yù)第14天:陽明經(jīng)組夾脊穴組對照組,陽明經(jīng)組表達(dá)量:第7天第14天,夾脊穴組表達(dá)量:第7天第14天,對照組表達(dá)量:第7天第14天,VEGF的表達(dá)情況在第7天:夾脊穴組對照組陽明經(jīng)組,第14天:陽明經(jīng)組夾脊穴組對照組,陽明經(jīng)組表達(dá)量:第7天第14天,夾脊穴組表達(dá)量:第7天第14天,對照組表達(dá)量:第7天第14天。結(jié)論:電針可以通過提高受損脊髓節(jié)段HIF-1a及VEGF的蛋白和mRNA的表達(dá)來實(shí)現(xiàn)對SCI后功能障礙的恢復(fù)干預(yù)作用。電針刺激足陽明胃經(jīng)對SCI后功能障礙治療,最佳的介入時(shí)間為受損1周后。
[Abstract]:Aim: to study the effect of electroacupuncture on the expression of HIF-1a and VEGF in injured spinal cord in rats. Methods: the spinal cord injury model of female SD rats was established by spinal cord clamp method. On the 3rd day after the establishment of the model, the rats were randomly divided into three groups: Jiaji group, gastric meridian group and injury control group. Foot Yangming stomach meridian acupoint treatment and do not use electroacupuncture treatment. The neurological function of rats in each group was evaluated regularly by BBB scoring method. After 14 days of treatment, the rats were killed. The injured spinal cord of the rats in each group was taken out, and the damage of the spinal cord was observed by HE staining. The expression of HIF-1a and VEGF protein and the mRNA expression of HIF-1a and VEGF in each group were compared by immunohistochemical staining and qRT-PCR respectively. Results: before treatment, there was no significant difference in BBB functional score of rats in each group after 1 week of treatment. After 2 weeks of treatment, the BBB score: Yangming group with spinal cord injury control group, HE staining showed that the spinal cord injury in each group accorded with its functional score. Immunohistochemical staining showed that the expression of HIF-1a was observed on the 7th day of electroacupuncture intervention: the control group was treated with Yangming Meridian Jiaji acupoint group, the control group with electroacupuncture intervention on the 14th day, the Yangming Meridian group with Jiaji acupoint group, and the Yangming Meridian group with the expression level of Yangming Meridian Group on the 7th day on the 14th day. The expression of VEGF in Jiaji acupoint group on the 7th day and the control group on the 14th day: on the 7th day, the expression of VEGF was on the 7th day; in the control group, the Yangming meridian group was the Jiaji acupoint group, the 14th day was the Yangming meridian Jiaji acupoint group, the control group was the Yangming meridian Jiaji acupoint group. The expression of Yangming Meridian on the 14th day, Jiaji acupoint group on the 14th day, the control group on the 14th day and on the 14th day on the 7th day. QRT-PCR results showed that the expression of HIF-1a was on the 7th day after electroacupuncture intervention: the Jiaji acupoint group was the control group, and the control group was Yangming meridian group. The 14th day of electroacupuncture intervention: Yangming meridian group Jiaji point group control group, Yangming meridian group expression amount: the 7th day 14 days, Jiaji point group expression amount: the 7th day the 14th day; The expression of VEGF in the control group: on the 7th day, the expression of VEGF was on the 7th day: Jiaji acupoint group, the control group, the Yangming meridian group, the 14th day: the Yangming meridian group, the control group, and the Yangming meridian group on the 7th day and the 14th day, respectively. The expression level of Jiaji acupoint group was 14 days on the 7th day, and that of the control group was 14 days on the 7th day. Conclusion: electroacupuncture can improve the expression of HIF-1a and VEGF protein and mRNA in injured spinal cord segment to improve the recovery of SCI. The best interventional time was 1 week after injury after SCI was treated by electroacupuncture stimulation of foot Yangming stomach Meridian.
【學(xué)位授予單位】：廣西中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245

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