【摘要】：目的:研究植物蛋白和動(dòng)物蛋白對(duì)氣陰兩虛2型糖尿病大鼠氨基酸代謝譜的影響。方法:將110只雄性SD大鼠隨機(jī)分為空白組(10只),糖尿病模型組(20只),病癥結(jié)合組(80只),正常組大鼠給予普通飼料,其余給予高糖高脂飼料全程喂養(yǎng),期間病癥結(jié)合組灌服青皮附子(15.75 g/kg)每日一次,5w后,腹腔注射小劑量STZ(40 mg/kg)建立實(shí)驗(yàn)性糖尿病大鼠模型(正常組除外),將病癥結(jié)合成模的大鼠按照血糖隨機(jī)分為氣陰兩虛糖尿病模型組(附子青皮,15.75 g/kg),大豆蛋白組(大豆蛋白,0.5g/kg),牛奶蛋白組(牛奶蛋白,0.5 g/kg),黃連素組(黃連素,0.1 g/kg),連續(xù)灌胃給藥4周。蛋白干預(yù)4周后,腹主動(dòng)脈采血分離其血清,1.運(yùn)用BS-120全自動(dòng)生化儀檢測(cè)大生化指標(biāo)。2.運(yùn)用1H-NMR尋求對(duì)體內(nèi)特征性代謝物。3.運(yùn)用AQC柱前衍生高效液相色譜法,利用熒光檢測(cè)器對(duì)血清中游離氨基酸進(jìn)行分析。結(jié)果:蛋白干預(yù)4周后,大豆蛋白、牛奶蛋白與黃連素對(duì)2型糖尿病大鼠體重和血糖無(wú)明顯影響。1.與氣陰兩虛糖尿病組比較,大豆蛋白組總蛋白(TP)、球蛋白(GLB)、血肌酐(Crea)、尿蛋白(Pro)明顯升高(P0.05),尿素氮/肌酐(BUN/CREA)、尿素(Urea)顯著降低(P0.05),牛奶蛋白組BUN/CREA顯著降低(P0.05)。2.與氣陰兩虛DM模型組比較,大豆蛋白組乳酸、肌酸明顯降低(VIP3),α-葡萄糖明顯增加且均有顯著性差異(VIP3),牛奶蛋白組乳酸明顯降低(VIP3),α-葡萄糖明顯增加且均有顯著性差異(VIP3),黃連素組β-羥異丁酸、乳酸、肌酸明顯降低(VIP3),巖藻糖、α-葡萄糖明顯增加且均有顯著性差異(VIP3)。3與氣陰兩虛糖尿病模型組比較,大豆蛋白組明顯增加丙氨酸、甘氨酸、天冬氨酸、脯氨酸、絲氨酸、谷氨酸、精氨酸、酪氨酸、蛋氨酸、異亮氨酸+亮氨酸、蘇氨酸(r0,且VIP1),牛奶蛋白組明顯增加丙氨酸、酪氨酸、脯氨酸、蘇氨酸、蛋氨酸、苯丙氨酸、賴(lài)氨酸、組氨酸、纈氨酸、異亮氨酸+亮氨酸、絲氨酸、甘氨酸(r0,且VIP1),黃連素組明顯增加丙氨酸、異亮氨酸+亮氨酸、絲氨酸、牛磺酸、甘氨酸、蛋氨酸、精氨酸、賴(lài)氨酸、脯氨酸、組氨酸、苯丙氨酸、纈氨酸、谷氨酸(r0,且VIP1)。結(jié)論:蛋白灌胃對(duì)氣陰兩虛2型糖尿病SD大鼠氨基酸代謝能產(chǎn)生了一定的影響,對(duì)肝、腎功能具有一定的保護(hù)作用。通過(guò)代謝組學(xué)技術(shù)找到標(biāo)志性差異化合物,從而為2型糖尿病早期預(yù)警和氣陰虛證侯提供實(shí)驗(yàn)依據(jù)。
[Abstract]:Aim: to study the effect of plant protein and animal protein on amino acid metabolism in type 2 diabetic rats with deficiency of qi and yin. Methods: 110 male SD rats were randomly divided into control group (n = 10), diabetic model group (n = 20) and disease combination group (n = 80). During the treatment group, aconite was administered once a day (15.75 g/kg), 5 weeks later, An experimental diabetic rat model was established by intraperitoneal injection of small dose STZ (40 mg/kg) (except the normal group). The rats combined with the disease were randomly divided into two groups according to their blood glucose: Qingpi group (qi-yin deficiency, 15.75 g/kg) and soybean protein group (Daqing g/kg). Soybean protein (0.5g/kg), milk protein group (milk protein, 0. 5 g/kg) and berberine group (0. 1 g/kg) were given orally for 4 weeks. After 4 weeks of protein intervention, blood samples from abdominal aorta were collected and their serum was isolated. Using BS-120 automatic biochemical instrument to detect large biochemical indexes. 2. 1H-NMR was used to search for the characteristic metabolites in vivo. The free amino acids in serum were determined by AQC precolumn derivatization HPLC and fluorescence detector. Results: after 4 weeks of protein intervention, soybean protein, milk protein and berberine had no significant effects on body weight and blood glucose of type 2 diabetic rats. 1. Compared with the diabetic group with deficiency of qi and yin, the total protein (TP), globulin (GLB), creatinine (Crea), urinary protein (Pro) in soybean protein group was significantly increased (P0.05), urea nitrogen / creatinine (BUN/CREA), urea (Urea) significantly decreased (P0.05), and BUN/CREA in milk protein group was significantly decreased (P0.05). Compared with the DM model group of deficiency of qi and yin, soybean protein group lactic acid, Creatine (VIP3), 偽 -glucose (VIP3), lactate (VIP3), 偽 -glucose (VIP3), beta-hydroxyisobutyric acid (尾 -hydroxyisobutyric acid), lactic acid (lactic acid) were significantly increased in milk protein group. The levels of creatine (VIP3), alginate, 偽 -glucose and alanine, glycine, aspartic acid, proline, serine, glutamate were significantly increased in the soybean protein group compared with the diabetic model group with deficiency of qi and yin (VIP3), and the levels of alanine, glycine, aspartic acid, proline, serine and glutamate were significantly increased in the soybean protein group. Alanine, tyrosine, proline, threonine, methionine, phenylalanine, lysine, histidine, valine were significantly increased in the arginine, tyrosine, methionine, isoleucine leucine, threonine (r0, and VIP1) groups. Isoleucine leucine, serine, glycine (r0, and VIP1), berberine group significantly increased alanine, isoleucine leucine, serine, taurine, glycine, methionine, arginine, lysine, proline, histidine, phenylalanine, Valine, glutamic acid (r0, and VIP1). Conclusion: protein gavage has a certain effect on amino acid metabolism in type 2 diabetic rats with deficiency of qi and yin, and has protective effect on liver and kidney function. In order to provide experimental basis for early warning of type 2 diabetes mellitus and deficiency of qi and yin syndrome, the marked differential compounds can be found by metabonomics technique.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R259

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