【摘要】：目的:觀察壯醫(yī)藥線點(diǎn)灸療法對(duì)痛經(jīng)模型大鼠的療效及對(duì)一氧化氮(NO)、內(nèi)皮素-1(ET-1)的影響,探討其對(duì)該痛經(jīng)模型治療的可能作用機(jī)制。方法:選取12周齡雌性SD大鼠110只隨機(jī)分為正常對(duì)照組(A組)、痛經(jīng)模型組(B組)、壯醫(yī)藥線點(diǎn)灸組(C組)、月月舒藥物組(D組)、針刺組(E組),每組22只。采用雌激素+腎上腺素注射并全身冰水浸泡法造模,觀察各組大鼠注射縮宮素30min內(nèi)扭體次數(shù)和扭體反應(yīng)潛伏期,分別用NO還原酶法及ELISA法測(cè)定大鼠血漿、子宮勻漿中NO、ET-1水平。結(jié)果:1、與對(duì)照組比較,模型組扭體潛伏期短,扭體次數(shù)最多,有顯著統(tǒng)計(jì)學(xué)意義(P0.01);壯醫(yī)藥灸組、針刺組以及藥物組與模型組相比,首次扭體潛伏期均明顯延長(zhǎng),且30 min內(nèi)扭體反應(yīng)次數(shù)少(P0.01);壯醫(yī)藥灸組與藥物組、針刺組在30min內(nèi)扭體次數(shù)相比較,均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);但壯醫(yī)藥灸組與藥物組相比,其扭體潛伏期明顯伏延長(zhǎng)(P0.05);且三組中,壯醫(yī)藥灸組扭體潛伏期最長(zhǎng)、扭體抑制率最高。2、與正常組比較,壯醫(yī)藥灸組、針刺組、藥物組大鼠血清及子宮勻漿NO、ET-1含量無(wú)顯著差異(P0.05);與模型組比較,壯醫(yī)藥灸組、針刺組及藥物組血清及子宮勻漿ET-1顯著降低,NO明顯升高(P0.01);壯醫(yī)藥灸組、針刺組以及藥物組相比,血清及子宮勻漿NO、ET值均無(wú)明顯統(tǒng)計(jì)學(xué)差異(P0.05);結(jié)論:壯醫(yī)藥線點(diǎn)灸療法治療寒濕凝滯型痛經(jīng)的作用機(jī)制可能與增加大鼠血清及子宮組織中NO水平、降低血清及子宮組織ET-1水平有關(guān)。
[Abstract]:Objective: to observe the effect of Zhuang medicine thread moxibustion on dysmenorrhea model rats and the effect of nitric oxide (NO), endothelin-1 (ET-1) on dysmenorrhea model. Methods: one hundred and ten female SD rats aged 12 weeks were randomly divided into 4 groups: normal control group (group A), dysmenorrhea model group (group B), moxibustion group (group C), moxibustion group (group D) and acupuncture group (group E). The rats were injected with estrogen epinephrine and soaked in ice water all over the body. The times of writhing and the latency of writhing reaction were observed by injection of oxytocin 30min. The levels of NO,ET-1 in plasma and uterine homogenate were measured by NO reductase method and ELISA method respectively. Results: 1, compared with the control group, the model group had shorter latent period of writhing and the most times of writhing (P0.01), and the first writhing latency of the Zhuang medicated moxibustion group, acupuncture group and drug group was significantly longer than that of the model group. The times of writhing reaction in 30 min were less (P0.01); the times of writhing in 30min were not significantly different between Zhuang medicinal moxibustion group and drug group and acupuncture group (P0.05); but the latent period of writhing in Zhuang medicinal moxibustion group was significantly longer than that in drug group (P0.05); in the three groups, the writhing latency of Zhuang medicinal moxibustion group was significantly longer than that of drug group (P0.05). The latent period of writhing was the longest and the inhibition rate of writhing was the highest in Zhuang medicinal moxibustion group. 2. There was no significant difference in NO,ET-1 content in serum and uterus homogenate of rats in Zhuang medicinal moxibustion group, acupuncture group and drug group (P0.05), compared with model group, Zhuang medicated moxibustion group had no significant difference (P0.05). ET-1 in serum and uterine homogenate in acupuncture group and drug group were significantly decreased and NO was significantly increased (P0.01), while that in Zhuang medicinal moxibustion group, acupuncture group and drug group was significantly higher than that in acupuncture group and drug group. There was no significant difference in NO,ET values between serum and uterine homogenate (P0.05). Conclusion: the mechanism of treating dysmenorrhea with cold dampness and stagnation may be related to the increase of NO level in serum and uterine tissue of rats. The decrease of ET-1 level in serum and uterus was related.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R29

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