秩邊透水道技術(shù)對原發(fā)性痛經(jīng)模型大鼠T淋巴細(xì)胞亞群和前列腺素的影響
發(fā)布時間：2018-10-10 06:36
【摘要】：目的:1、觀察分析秩邊透水道技術(shù)治療原發(fā)性痛經(jīng)模型大鼠的療效及其鎮(zhèn)痛作用,并與現(xiàn)代常用針刺方法相比較,觀察兩種治療方法的療效差別。為針灸治療原發(fā)性痛經(jīng)提供多元化的治療方法。2、觀察秩邊透水道技術(shù)對原發(fā)性痛經(jīng)模型大鼠外周血T淋巴細(xì)胞亞群CD3~+、CD4~+的百分?jǐn)?shù)及CD4/CD8比值、對大鼠子宮內(nèi)膜前列腺素E2和F2α的含量、對痛經(jīng)模型大鼠胸腺、脾臟的病理變化的影響,從免疫學(xué)角度,探討秩邊透水道技術(shù)對原發(fā)性痛經(jīng)的特異性治療作用,為臨床應(yīng)用和推廣提供可靠的實(shí)驗(yàn)數(shù)據(jù)和科學(xué)依據(jù),從而更好的指導(dǎo)臨床。方法:將40只健康清潔級雌性未孕SD大鼠隨機(jī)分為4組,每組十只。分為空白對照組、模型組、針刺組、秩邊透水道組。具體操作方法遵從文獻(xiàn)方法并加以改進(jìn),四組同期進(jìn)行。首先在模型組,針刺組,秩邊透水道組大鼠股部皮下注射苯甲酸雌二醇10天,每日1次,第1天0.5mg/只,第2-9天0.2mg/只,第10天0.5mg/只,第11天腹腔注射催產(chǎn)素2u/只。對照組大鼠股部皮下注射同等計量的生理鹽水10天,每日1次,第11天腹腔注射生理鹽水0.2ml/只。觀察記錄腹腔注射藥物后30分鐘內(nèi)大鼠扭體反應(yīng)發(fā)生的次數(shù)。采用流式細(xì)胞儀檢測法觀察各組大鼠外周血T淋巴細(xì)胞亞群CD3~+、CD4~+、CD8~+的百分?jǐn)?shù),采用放射免疫法檢測各組大鼠子宮內(nèi)膜前列腺素E2及F2α的含量,采用HE染色法觀察各組大鼠胸腺、脾臟的病理變化。結(jié)果:1、腹腔注射藥物后30min內(nèi),空白對照組沒有出現(xiàn)扭體反應(yīng),其余三組則均出現(xiàn)不同程度的扭體反應(yīng),以模型組扭體次數(shù)最多,與空白對照組比較,差異有統(tǒng)計學(xué)意義(P0.05),說明本次實(shí)驗(yàn)造模成功。針刺組和秩邊透水道組扭體次數(shù)明顯減少,與模型組比較有顯著差異,且差異有統(tǒng)計學(xué)意義(P0.05)。說明此兩組都能夠有效的治療原發(fā)性痛經(jīng),減輕扭體次數(shù)的發(fā)生。2、與空白對照組比較,模型組大鼠子宮內(nèi)膜PGF2α含量明顯升高,PGE含量明顯降低,PGF2α/PGE2比值明顯升高,差異有統(tǒng)計學(xué)意義(P0.05),從病因角度說明造模成功;針刺組、秩邊透水道組與模型組相比較,PGF2α含量明顯降低,PGE2含量明顯升高,PGF2α/PGE2比值降低,差異有統(tǒng)計學(xué)意義(P0.05),說明針刺組和秩邊透水道組對原發(fā)性痛經(jīng)有鎮(zhèn)痛作用。3、與空白對照組比較,模型組CD3~+、CD4~+、CD8~+水平均出現(xiàn)明顯變化,CD3~+、CD4~+水平以及CD4/CD8水平降低,差異有統(tǒng)計學(xué)意義(P0.05),針刺組、秩邊透水道組與模型組相比,CD3~+、CD4~+及CD4/CD8水平上升,趨向于空白對照組T淋巴細(xì)胞亞群水平,差異有統(tǒng)計學(xué)意義(P0.05)。說明針刺組和秩邊透水道組都能夠調(diào)節(jié)痛經(jīng)模型大鼠的免疫功能。4、針刺組和秩邊透水道組各個指標(biāo)的比較差異均無統(tǒng)計學(xué)意義(P0.05),說明此次實(shí)驗(yàn)不能有效的證明傳統(tǒng)針刺法與秩邊透水道法治療效果的優(yōu)劣。5、胸腺、脾臟在病理變化方面,模型組均發(fā)生了明顯的病理變化,而針刺組和秩邊透水道組的胸腺、脾臟的病理變化程度較模型組輕微。從病理學(xué)角度說明了秩邊透水道技術(shù)對原發(fā)性痛經(jīng)免疫系統(tǒng)的影響。結(jié)論:1、秩邊透水道技術(shù)對原發(fā)性痛經(jīng)有鎮(zhèn)痛作用。2、秩邊透水道技術(shù)在一定程度上能夠調(diào)節(jié)痛經(jīng)模型大鼠的免疫功能。
[Abstract]:Objective: 1. To observe the curative effect and its analgesic effect in the treatment of primary dysmenorrhea model rats. To provide a method for the treatment of primary dysmenorrhea by acupuncture and moxibustion. 2. The percentage of CD3 +, CD4 + and CD4/ CD8 in peripheral blood T lymphocytes in the peripheral blood T lymphocytes of primary dysmenorrhea model rats were studied by using the technique of rank-edge pervious channel. The contents of prostaglandin E2 and F2 in the endometrium of rats were determined. In order to provide reliable experimental data and scientific basis for clinical application and promotion, we can better guide the clinical application. Methods: Forty healthy clean-grade female non-pregnant SD rats were randomly divided into 4 groups, each group was 10 rats. It is divided into blank control group, model group, acupuncture group, rank-edge pervious channel group. The specific operation method complies with the literature method and improves it, and the four groups are carried out in the same period. In the model group, the acupuncture group and the rat femoral component were injected with estradiol benzoate 10 days, once daily, day 1 0. 5mg/ day, day 2-9 day 0. 2mg/ day, 10th day 0. 5mg/ day, and oxytocin 2u/ day was injected intraperitoneally on day 11. Rats in the control group were injected with normal saline for 10 days at the same time, once every day, and normal saline was injected into the abdominal cavity at day 11 for 0. 2ml/ day. The number of torsion body reactions in rats after intraperitoneal injection was observed for 30 minutes. The percentages of CD3 ~ +, CD4 ~ + and CD8 ~ + in peripheral blood T lymphocytes in each group were observed by flow cytometry. The contents of prostaglandin E2 and F2 in the endometrium of rats were detected by radioimmunoassay. The thymus of each group was observed by HE staining. Pathological changes of spleen. Results: 1. In 30 minutes after intraperitoneal injection, no torsion reaction occurred in the blank control group. The other three groups showed a different degree of torsion body response. The number of torsion bodies in the model group was the largest, and the difference was statistically significant compared with the blank control group (P0.05). There was a significant difference in the number of torsion bodies in the acupuncture group and the group with the rank side, and the difference was statistically significant (P0.05). 2. Compared with the blank control group, the content of PGF2 in the endometrium of the model group was obviously increased, the content of NPY was obviously decreased, and the ratio of PF2 and PF2 was significantly higher than that in the blank control group (P <0.05). Compared with the model group, the content of PGF2 was significantly lower than that in the model group (P <0.05). It was indicated that the acupuncture group and the rank-side water-permeable waterway group had an analgesic effect on primary dysmenorrhea. Compared with the blank control group, the levels of CD3 +, CD4 + and CD8 ~ + in the model group were significantly changed, and the levels of CD3 +, CD4 + and CD4/ CD8 were decreased, and the difference was statistically significant (P0.05). Compared with the model group, the level of CD3 +, CD4 ~ + and CD4/ CD8 increased in the acupuncture group, and the difference was statistically significant (P <0.05). The results showed that both the acupuncture group and the rank-side water-permeable waterway group were able to adjust the immune function of the rats with dysmenorrhea model. The pathological changes of the thymus and spleen were observed in the model group. The pathological changes of the spleen were lower than that in the model group. From the pathological point of view, the influence of the technique on the immune system of primary dysmenorrhea was described. Conclusion: 1. The technique of rank-edge water-permeable waterway has analgesic effect on primary dysmenorrhea. The technique of rank-edge water-permeable waterway can regulate the immune function of menorrheal model rats to some extent.
【學(xué)位授予單位】：山西中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245
[Abstract]:Objective: 1. To observe the curative effect and its analgesic effect in the treatment of primary dysmenorrhea model rats. To provide a method for the treatment of primary dysmenorrhea by acupuncture and moxibustion. 2. The percentage of CD3 +, CD4 + and CD4/ CD8 in peripheral blood T lymphocytes in the peripheral blood T lymphocytes of primary dysmenorrhea model rats were studied by using the technique of rank-edge pervious channel. The contents of prostaglandin E2 and F2 in the endometrium of rats were determined. In order to provide reliable experimental data and scientific basis for clinical application and promotion, we can better guide the clinical application. Methods: Forty healthy clean-grade female non-pregnant SD rats were randomly divided into 4 groups, each group was 10 rats. It is divided into blank control group, model group, acupuncture group, rank-edge pervious channel group. The specific operation method complies with the literature method and improves it, and the four groups are carried out in the same period. In the model group, the acupuncture group and the rat femoral component were injected with estradiol benzoate 10 days, once daily, day 1 0. 5mg/ day, day 2-9 day 0. 2mg/ day, 10th day 0. 5mg/ day, and oxytocin 2u/ day was injected intraperitoneally on day 11. Rats in the control group were injected with normal saline for 10 days at the same time, once every day, and normal saline was injected into the abdominal cavity at day 11 for 0. 2ml/ day. The number of torsion body reactions in rats after intraperitoneal injection was observed for 30 minutes. The percentages of CD3 ~ +, CD4 ~ + and CD8 ~ + in peripheral blood T lymphocytes in each group were observed by flow cytometry. The contents of prostaglandin E2 and F2 in the endometrium of rats were detected by radioimmunoassay. The thymus of each group was observed by HE staining. Pathological changes of spleen. Results: 1. In 30 minutes after intraperitoneal injection, no torsion reaction occurred in the blank control group. The other three groups showed a different degree of torsion body response. The number of torsion bodies in the model group was the largest, and the difference was statistically significant compared with the blank control group (P0.05). There was a significant difference in the number of torsion bodies in the acupuncture group and the group with the rank side, and the difference was statistically significant (P0.05). 2. Compared with the blank control group, the content of PGF2 in the endometrium of the model group was obviously increased, the content of NPY was obviously decreased, and the ratio of PF2 and PF2 was significantly higher than that in the blank control group (P <0.05). Compared with the model group, the content of PGF2 was significantly lower than that in the model group (P <0.05). It was indicated that the acupuncture group and the rank-side water-permeable waterway group had an analgesic effect on primary dysmenorrhea. Compared with the blank control group, the levels of CD3 +, CD4 + and CD8 ~ + in the model group were significantly changed, and the levels of CD3 +, CD4 + and CD4/ CD8 were decreased, and the difference was statistically significant (P0.05). Compared with the model group, the level of CD3 +, CD4 ~ + and CD4/ CD8 increased in the acupuncture group, and the difference was statistically significant (P <0.05). The results showed that both the acupuncture group and the rank-side water-permeable waterway group were able to adjust the immune function of the rats with dysmenorrhea model. The pathological changes of the thymus and spleen were observed in the model group. The pathological changes of the spleen were lower than that in the model group. From the pathological point of view, the influence of the technique on the immune system of primary dysmenorrhea was described. Conclusion: 1. The technique of rank-edge water-permeable waterway has analgesic effect on primary dysmenorrhea. The technique of rank-edge water-permeable waterway can regulate the immune function of menorrheal model rats to some extent.
【學(xué)位授予單位】：山西中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245
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