發(fā)布時間：2018-08-26 21:37

【摘要】：目的:探討桂枝附子湯對膠原誘導(dǎo)性關(guān)節(jié)炎(CIA)大鼠滑膜組織中絲裂原活化蛋白激酶(MAPK)信號通路的影響。方法:將SD大鼠60只隨機(jī)分為正常組,模型組,桂枝附子湯組(1.5,4.5,13.5 g·kg~(-1)),甲氨喋呤組,共6組;大鼠尾根部皮下注射雞Ⅱ型膠原(chicken typeⅡcollagen,CCⅡ)乳劑制備CIA模型;自造模后第14天開始,每周觀察各給藥組的關(guān)節(jié)炎指數(shù);采用蛋白質(zhì)免疫印跡(Western blot)檢測滑膜組織MAPK信號轉(zhuǎn)導(dǎo)通路中JNK,ERK,p38蛋白及相應(yīng)磷酸化蛋白p-JNK,p-ERK,p-p38的表達(dá)水平。結(jié)果:CIA大鼠的足爪腫脹度和關(guān)節(jié)炎指數(shù)第7,14,21,28,35,42天個時間點均高于正常組,在給予桂枝附子湯治療后在第14天后CIA大鼠的足爪腫脹度和關(guān)節(jié)炎指數(shù)均明顯降低。桂枝附子湯能明顯改善CIA大鼠滑膜增生和炎性細(xì)胞浸潤,使得炎性細(xì)胞減少。與正常組p-JNK,p-ERK,p-p38比較,CIA組中的p-JNK,p-ERK,p-p38的表達(dá)水平顯著增強(qiáng),兩組治療間有顯著性變化(P0.01);用甲氨蝶呤治療CIA大鼠,與模型組比較其p-JNK,p-ERK和p-p38表達(dá)水平顯著降低,兩組治療間有顯著性變化(P0.01);用桂枝附子湯治療CIA大鼠,與模型組相比較其p-JNK,p-ERK,p-p38明顯降低,并且呈現(xiàn)劑量依賴性關(guān)系。結(jié)論:桂枝附子湯可抑制CIA大鼠MAPK信號轉(zhuǎn)導(dǎo)通路的活化,從而緩解炎癥癥狀。
[Abstract]:Aim: to investigate the effect of Guizhi Fuzi decoction on mitogen-activated protein kinase (MAPK) signaling pathway in synovium of collagen-induced arthritis (CIA) rats. Methods: sixty SD rats were randomly divided into normal group, model group and Guizhi aconite decoction group (1.54.5g kg~ (1), methotrexate group, 6 groups). The CIA model was induced by subcutaneous injection of chicken collagen type 鈪，

本文編號：2206234